276 research outputs found
Synthetic TDR Measurements for TEM and GTEM Cell Characterization
No full textThis paper describes the main features of the timedomain reflectometry (TDR) measurement technique and, in particular, the TDR analysis performed using a proper operating mode of the vector network analyzer (VNA), which is called synthetic TDR. Furthermore, some results of reflection measurement, which aim to characterize the impedance behavior of transverse electromagnetic (TEM) and gigahertz TEM cells by means of a commercial VNA in time-domain mode, are presented
"Tardività-ingiustificatezza" e "tardività-vizio procedimentale" del licenziamento disciplinare e relative sanzioni
No full textL'articolo approfondisce i seguenti argomenti: la “tardività-ingiustificatezza” dell’atto del licenziamento: la fattispecie della tolleranza; la “tardività-ingiustificatezza” del licenziamento derivante dalla tardività della contestazione dell’addebito; la tardività della contestazione disciplinare come vizio procedimentale; le sanzioni applicabili nell’area dell’art. 18 Stat. lav. alla “tardività-vizio procedimentale” e alla “tardività-ingiustificatezza” nell’area della tutela obbligatoria; le sanzioni applicabili alla “tardività-vizio procedimentale e i problemi di costituzionalità e le sanzioni per la “tardività-ingiustificatezza”, tra 4 e 5 comma art. 18. Stat. lav
Il licenziamento inefficace per vizio di forma
No full textL'articolo approfondisce i seguenti argomenti: il licenziamento orale e stessa tutela reale per tutti; l’inefficacia “solo a parole”: la tutela indennitaria dimezzata per i vizi procedimentali; l'applicazione del comma 6 anche per la omessa procedura o la omessa motivazione; la tardività del licenziamento disciplinare tra vizio formale e ingiustificatezza; l'infondatezza dei dubbi di costituzionalità del comma 6 e l'assenza della previsione espressa della tutela solo indennitaria per i vizi procedimentali nell’area della tutela obbligatoria
Infezioni delle basse vie urinarie nel cane e nel gatto: valutazione dei fattori predisponenti, dei microrganismi coinvolti e della loro antibiotico-sensibilità
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Experience on proficiency testing in Italy with emphasis on radiated emission measurements from 30 MHz to 1 GHz
No full textDevelopment of an improved <it>Pseudoalteromonas haloplanktis </it>TAC125 strain for recombinant protein secretion at low temperature
No full textAbstract Background In a previous paper, we reported the accomplishment of a cold gene-expression system for the recombinant secretion of heterologous proteins in Pseudoalteromonas haloplanktis TAC125. This system makes use of the psychrophilic α-amylase from P. haloplanktis TAB23 as secretion carrier, and allows an effective extra-cellular addressing of recombinant proteins. However, Pseudoalteromonales are reported to secrete a wide range of extra-cellular proteases. This feature works against the efficiency of the cold-adapted secretion system, because of the proteolytic degradation of recombinant products. The aim of this study is the construction of a P. haloplanktis TAC125 mutant strain with reduced extra-cellular proteolytic activity. Results P. haloplanktis TAC125 culture medium resulted to contain multiple and heterogeneous proteases. Since the annotation of the Antarctic bacterium genome highlighted the presence of only one canonical secretion machinery, namely the Type II secretion pathway (T2SS), we have inactivated this secretion system by a gene insertion strategy. A mutant strain of P. haloplanktis TAC125 in which the gspE gene was knocked-out, actually displayed a remarkable reduction of the extra-cellular protease secretion. Quite interestingly this strain still retained the ability to secrete the psychrophilic amylase as efficiently as the wild type. Moreover, the decrease in extra-cellular proteolytic activity resulted in a substantial improvement in the stability of the secreted amylase-β-lactamase chimera. Conclusion Here we report a cell engineering approach to the construction of a P. haloplanktis TAC125 strain with reduced extra-cellular protease activity. The improved strain is able to secrete the psychrophilic α-amylase (the carrier of our recombinant secretion system), while it displays a significant reduction of protease content in the culture medium. These features make the gspE mutant an improved host with a remarkable biotechnological potential in recombinant protein secretion at low temperature. Moreover this work demonstrates that P. haloplanktis TAC125 is a versatile psychrophilic host for recombinant protein production since it can be easily improved by a directed engineering approach. To the best of our knowledge, this is the first described example of a strain improvement strategy applied to an Antarctic bacterium.</p
Adipocyte expression and circulating levels of leptin increase in both gynaecological and breast cancer patients
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