192 research outputs found
Isolation process for mitochondrial transplantation
No full textMitochondrial transplantation is an innovative therapeutic approach for the treatment of mitochondrial dysfunction associated with a variety of metabolic, neurodegenerative and cardiovascular diseases. In this procedure, healthy mitochondria are isolated and transferred into damaged or dysfunctional cells to restore normal cellular bioenergetics. Recent advances have shown the feasibility of mitochondrial transplantation in preclinical and clinical settings, demonstrating its potential to improve outcomes in conditions such as myocardial infarction, neurodegenerative disorders, and acute kidney injury. In this contest, the isolation method for mitochondrial transplantation is crucial. High quality, pure mitochondria are essential to ensure that the transplanted organelles function correctly and integrate well into the recipient cells. In addition, efficient isolation techniques are required to obtain a sufficient number of mitochondria for transplantation. Low yield methods may not provide enough mitochondria for effective treatment. Several isolation techniques are currently used, including differential centrifugation, density gradient centrifugation and fractionated mitochondrial magnetic separation. Each method has its own advantages and limitations, and the choice of method often depends on the specific requirements of the application, such as the type of cells targeted and the disease to be treated. A key factor to consider when choosing the isolation method is the isolation time. Rapid isolation methods are preferred for the isolation of mitochondria from tissue biopsies. This review summarizes the current state of research on mitochondrial transplantation, highlights the main methods for isolating mitochondria and discusses their potential clinical applications in the field of regenerative medicine
Alloprevotella rava isolated from a mixed infection of an elderly patient with chronic mandibular osteomyelitis mimicking oral squamous cell carcinoma
No full textThe anaerobic, Gram-negative bacillus Alloprevotella rava has recently been described in the human oral cavity. To our knowledge, this species has not been isolated from chronic osteomyleitis samples. We present the first case of A. rava infection in a 92-year-old woman with polymicrobial chronic mandibular osteomyelitis, mimicking oral squamous cell carcinoma. (c) 2021 The Author(s). Published by Elsevier Ltd
Storage of isolated mitochondria
No full textAfter extraction and quantification steps, the most intriguing challenge in preparing mitochondria for transplantation of these organelles is the preservation of the material isolated from different tissues. It is essential to maintain these organelles in a viable condition without losing the biological characteristics of the tissue of origin. After isolation and quantification, various methods, when performed following proper protocols, lead to the last step of preparation mitochondria ready for use. Two main methods are used for preservation of mitochondria after isolation: cold storage and cryopreservation. Current methodological approaches in mitochondrial transplantation techniques suggest using freshly prepared isolated mitochondria from different tissues; this could be a limitation for mitochondrial transplantation techniques, and further efforts in methodological research should be aimed at overcoming this problem
A study on dynamic modeling to examine the impact of reducing water pollution on market condition in the Turkish Black Sea Basin
No full textThe Black Sea has severe water pollution problems during the last three decades due to tremendous loads of different types of pollutants discharged from the entire basin Therefore, finding solutions to improve the environmental status of the Black Sea is one of the critical issues. Previous paper, we analyzed Turkish black Sea Basin using a conceptual mathematical model describes the interaction between the socioeconomic activities and behavior of the pollutants starting from the contamination sources ending to the sea. As a results, set of mix policies was introduced to reduce the water pollution generate from Turkish Black Sea Basin. In this paper, we discuss the implementation of such approach on formulating a comprehensive policy to reduce the water pollution from the whole Black Sea basin in order to improve the water quality of The Black Sea.
The creative training in the visual arts education
No full textThe main purpose of this study was to examine the effect of creative training on creative thinking and problem solving of students in visual arts higher education. The participants were Turkish university students. The findings indicated that the effect of creative training on students' creative thinking was significant and effect size medium. However, creative training was not effective significantly on problem solving of students. The results indicate that the creative training as educational approach is effective on the creative thinking, but it is not effective on problem solving of students. Accordingly, it was concluded that open structures in the learning activities as component of creative training, can be cause of open-ended thinking in the thinking process. This result suggests that open structures as 'open-ended thinking' and open-minded in learning activities with the liberatedflexible learning environment and teacher's encouragements plays important role in the development creative thinking and problem solving of students. (C) 2015 Elsevier Ltd. All rights reserved
A COMPARISON STUDY FROM A CREATIVE THINKING PERSPECTIVE IN DIFFERENT DOMAINS AS ART EDUCATION AND NON-ART EDUCATION STUDENTS
No full textCreative thinking measurement for everyone in different domains is the most crucial critical subject. Therefore, the current discussion continues whether creativity in various fields different. This study aimed to measurement the students’ creative thinking scores in various domains disciplines to reveal differences. The main research question was as followed: Do whether students’ creative thinking scores of students in art and non-art education differ significantly? The method was a causal-comparative research design. Participant students (N = 456, meanage = 16–21) were equivalent regarding some variations; as education disciplines and ages. While however, the art and non-art education students were the domain-specific was art education students, the and domaingeneral, was non-art education students respectively. The present study determined a significant difference between domain-specific and domain-general on creative thinking scores in favor of the non-art education students in the range of ages 16 to 21. The study concluded that alternative instruments with domain-specific content needed to measure individuals’ creative thinking in the domain-specific. This study suggested future research to conduct creativity measurement comparatively individuals in different age ranges and domains fields. © 2023 The Author(s). Published by Vilnius Gediminas Technical University
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