1,721,248 research outputs found
Serological response to vaccination against Aujeszky’s disease with a needle-free injector
No full textAujeszky’s disease or pseudorabies is still present in
Italy. One of the major tools to control and eradicate the
disease is the systematic vaccination of swine with gEdeleted
modified live vaccines. Needle-free injection
of vaccines prevents residual needle fragments and
associated meat defects from the injection site.
AKIPOR® 6.3 (Merial, Lyon, France) is a freezed dried
modified live vaccine (gE-deleted Bartha strain) with an
oily adjuvant against Aujeszky’s disease. This study
aimed to compare the serological response to different
vaccination programs either combining the
recommended intra-muscular (IM) administration route
of AKIPOR® 6.3 to intra-dermal (ID) vaccine
administration using a needle-free injection device or
combining only ID vaccine administrations.
Materials and Methods
The trial was conducted in a 400-sow multi-site farrowto-
finish operation known to be gE-negative and raising
fatteners for Parma ham until 270-300 days of age. The
vaccination program against Aujeszky’s disease in force
on the farm includes a two-shot primo-immunization at
70 and 90 days of age in the pre-fattening period
followed by a booster injection at 180 days of age in the
fattening period.
A total of 36 pigs divided in 3 groups of 12 pigs was
vaccinated against Aujeszky’s disease according to the
treatment groups. Vaccine IM administrations were performed under a 2.0-
mL dose following reconstitution according to the
manufacturer recommendations (i.e. mixing 100 mL
O/W adjuvant mixed with a 50-dose freezed-dried virus
pellet).
Intra-dermal administrations were performed using a
needle-free injection device (VALERY® device,
equipped with a nose-piece adapted for 0.2 mL ID
injection, Giordano Poultry-Plast, Caraglio, Italy) under
a 0.2-mL dose prepared by mixing 10 mL O/W adjuvant
mixed with 50-dose freezed-dried virus powder.
Serum samples were collected from each pig before
primo-immunization, one month later and one month
following booster injection. Routine gE and gB ELISA
assays were conducted at IZSLER (Brescia, Italy). No adverse event was observed following vaccine
administration whatever the vaccination protocol. No seroconversion against gE protein did occur thus
confirming the absence of viral circulation in the pigs in
the conditions of the study. In this context, a clear
seroconversion against gB protein following primoimmunization
was evidenced following each vaccination
protocol with no difference evidenced between groups
(Kurskall-Wallis test, p>0.27). A clear increase in gB
antibody titer was also observed following the ID
booster whatever the vaccination protocol applied for the primo-immunization. The vaccination programs against Aujeszky’s disease
tested in this study included 2 to 3 ID injections with AKIPOR 6.3 at an adjusted dose in 0.2 mL O/W
adjuvant and appeared to be similarly potent to elicit a
satisfying serological response. These findings
corroborate previous results obtained in similar
conditions where only the booster injection was
administrated intra-dermally
Nucleotide sequences modulating the expression of genes in plants
Full text linkThe provides a genetic cassette for the expression of heterologous nucleic acids in response to abiotic stresses such as drought and soil high salinity, regulatory sequences used in the expression cassette, expression vectors carrying these sequences and plants transformed with the same
Insertional mutagenesis in Arabidopsis : a tool for functional genomic analysis
No full textThe completion of the entire Arabidopsis genome sequence has been recently achieved. Approximately 30% of the predicted genes encode for proteins of unknown function, and only 9% of the genes have been characterized experimentally. Different genetic and molecular tools have been developed to address the functional significance of the genes discovered in EST and genome sequencing programs. Bioinformatic studies can assign putative functions by homology to known genes, while microarray technology can examine global and detailed gene expression patterns. Nevertheless, the identification and characterization of loss-of-function mutations remains a primary tool in functional genomics studies. Insertional mutagenesis approaches are well suited for large-scale functional analysis. Insertions of transposons or T-DNA in specific target genes can be easily detected by reverse genetic PCR-assisted screens. The molecular and phenotypic characterization of the insertional alleles provide vital data on the biological role of the disrupted gene
Cassette for nucleic acid expression in plants
No full textThe present invention relates to the expression of recombinant nucleic acids in plants. More specifically the invention provides a promoter for the selective expression of genes in stomatal guard cells, gene constructs containing said promoter, expression vectors thereof and plants transformed therewith. The selective expression of genes in stomatal guard cells allows the regulation of their opening/closing states thereby modulating, e.g. increasing, the plant ability to resist to adverse environmental or climatic conditions
Cassetta per l’espressione ABA-indipendente di acidi nucleici ricombinanti nelle cellule di guardia delle piante
No full textSi intende risolvere il problema dell'espressione cellula-specifica di geni vegetali negli stomi attraverso l'utilizzo del promotore del gene CYP86A2 di Arabidopsis thaliana. Tale gene risulta espresso in modo specifico nelle cellule di guardia che delimitano l'apertura stomatica e presenti sull'epidermide della parte aerea dei vegetali. L'utilizzo del promotore di CYP86A2 consente di esprimere in modo costitutivo e specifico di geni esogeni in cellule di guardia, al fine di ridurre l'apertura della rima stomatica per limitare la perdita d'acqua dai tessuti vegetali. Il gene di Arabidopsis thaliana CYP86A2 è espresso in modo specifico nelle cellule di guardia degli stomi. Il putativo promotore di CYP86A2 è stato isolato e posto a monte del gene reporter GFP (costrutto pCYP86A2:GFP). Le piante transgeniche di Arabidopsis ottenute inseguito alla trasformazione con il costrutto pCYP86A2:GFP evidenziano espressione del reporter unicamente nelle cellule di guardia. Questo dimostra che tale promotore è in grado di guidare l'espressione dei geni posti sotto il suo controllo in modo specifico nelgi stomi. Il promotore di CYP86A2, rappresenta quindi un prezioso strumento per l'espressione stoma-specifica di geni regolatori, al fine di limitare la perdita d'acqua per traspirazione. Questa strategia consente di ottenere piante in grado di meglio tollerare eventi di carenza idrica, e di sostenere elevati livelli produttivi con un ridotto consumo d'acqua
- …
