219 research outputs found
Preliminary data on glycoconjugate histochemistry in the foetus and adult fallow deer duodenum
No full textProgetto di un robot parallelo a cinematica innovativa per operazioni di assemblaggio
No full textLa memoria illustra la progettazione di un robot a tre assi per moti di pura traslazione, da impiegare per operazioni di assemblaggio caratterizzate da elevate forze di interazione con l’ambiente: l’architettura innovativa della macchina ha reso lo stesso processo di progettazione non banale ed i risultati ottenuti sembrano interessanti. La macchina in questione presenta caratteristiche cinematiche molto buone, mentre le prestazioni dinamiche decadono velocemente nelle zone più esterne dello spazio di lavoro, il che è comunque compatibile con l’uso ipotizzato
The World of Adipokines: Their Presence and Distribution in the Organism and Their Relevance in Veterinary Medicine
No full textThe word “adipokines” generally identifies all the molecules synthesized and secreted by the adipose tissue. This evidence suggests that the adipose tissue can be no longer seen only as a storage tissue but also as a real “endocrine organ”. The adipokines carry out a local action (autocrine/paracrine) on the same producing tissue but also a systemic one (endocrine) on different target organs and tissues. The numerous studies conducted in this regard and the evidence of their distribution, often ubiquitous, with the presence of production sites additional to the adipose tissue, have allowed us to hypothesize and, in some cases, to demonstrate their involvement in the functional control of the organs where they have been highlighted.
Original manuscripts, review articles, and short communications and commentaries, which address any aspects of adipokines in domestic and wild animals, are invited for this special issue. Manuscripts that use a multidisciplinary approach and address any aspect of adipokines with a direct impact on animal welfare are particularly welcome
A lectin histochemical study of the oesophagus of shi drum
No full textThe saccharide composition of surface and secretion glycoconjugates in the oesophagus of Umbrina cirrosa was examined by means of lectin histochemistry. Mucous cells showed the presence of N-acetylgalactosamine, N-acetylglucosamine and sialic acid linked to the dimer galactosyl(β1→3) N-acetylgalactosamine. Columnar epithelial cells had a positive reaction with almost all the lectins employed, located in the supranuclear region and in the cell coat. The presence of abundant and various glycoconjugates in the secretions of shi drum oesophagus was correlated to the absence of salivary glands in fishes in general
Glycoconjugate distribution in gastric fundic mucosa of Umbrina cirrosa L. revealed by lectin histochemistry
No full textThe stomach of adult shi drum Umbrina cirrosa was investigated using a battery of nine horseradish peroxidase-conjugated lectins combined with enzymatic treatment, in order to distinguish glycoconjugate sugar residues. Epithelial cells showed the presence of galactosyl(β1→4)N- acetylglucosamine, mannose, N-acetylgalactosamine, N-acetylglucosamine, fucose and sialic acid-galactosyl(β1→3)N-acetylgalactosamine residues. Gastric pits had similar sugar residues with the exception of N-acetylgalactosamine which was less diffused. Gastric glands were characterized by the presence of glycoconjugates containing galactosyl(β1→3)N- acetylgalactosamine, N-acetylglucosamine, galactosyl(β1→4) N-acetylglucosamine, N-acetylgalactosamine and a small amount of sialic acid linked to N-acetylgalactosamine
Cythomorphological evaluation of horse adipose-derived mesenchymal stem cells after different storage conditions
No full textTransplantation of mesenchymal stem cells (MSCs) is a promising therapy and it
is known that, in the horse, these cells are already used for teno-desmic injuries treatment.
However, till now, knowledge about the MSC behaviour is scarce (Koch, Can.
Vet.J., 50, 2009) and, in particular, data on the effects of the cell manipulation before
transplantation are lacking.
In this work we studied MSCs maintained at different temperatures for different
periods of time simulating the phase of conservation before their use. Cryopreserved
MSCs were thawed, resuspended in a medium devoid of fetal calf serum (1x106cc/ml)
and divided in five aliquots. After a maintenance at 4°C or at room temperature for
0, 24 and 48 hours, MSCs were used for the following trials: cell viability, duplication
time, Colony-Forming Unit Assays, karyotype evaluation, adipogenic and osteogenic
differentiation, expression of MSC marker CD44 and CD90. Result evaluation seems to
suggest that all tested manipulation were unable to induce appreciable modifications
on MSC behaviour. All the aliquots were clonogenic, could differentiate along the osteogenic
and adipogenic lineage and expressed high levels of CD44 and CD90 antigens.
We suppose that the tested conditions of maintenance do not change the characteristics
of horse adipose-derived MSCs as regards the considered parameters
Different expression of AQP5 in sheep salivary glands related to forage water content during two vegetative cycles.
No full textStigma of Suicide Attempt (STOSA) scale and Stigma of Suicide and Suicide Survivor (STOSASS) scale: Two new assessment tools
No full textThis study aimed at validating two new assessment tools, the Stigma of Suicide Attempt (STOSA) scale and the Stigma of Suicide and Suicide Survivor (STOSASS) scale. The Devaluation-Discrimination scale of Link et al. was translated into Italian and adapted to measure stigma towards suicidal behavior. Both scales were administered to a mixed sample including members of the general population (n=282), patients with a mental disorder (n=113), suicide attempters (n=57) and people who had lost a significant other to suicide (n=75). Reliability of the scales was good in terms of both internal coherence and test-retest stability. Factor analysis produced an acceptable solution for the STOSA-scale. Items were distributed into two factors, one grouping items to measure supportive, respectful and caring attitudes, the other factor grouping items oriented towards stigmatizing attitudes and beliefs. The clinical populations were more inclined towards stigmatization of suicide than were people from the general population, who might be less aware of the stigma attached to suicide. The two scales may be helpful to quantify stigma at individual level in order to provide targeted supportive interventions, and at population level to measure changes in the beliefs and attitudes of the general population
Immunohistochemical evidence of Orexin-A in the pancreatic Beta cells of domestic animals.
No full textA large body of information proves that OrexinA is present in the pancreatic endocrine cells of humans and laboratory animals; more detailed studies identify Orexin-A-immunopositive cells as beta cells.
Because no data have been reported on the pancreas of domestic animals, we investigated the presence and the distribution of cells containing OrexinA in the pancreas of cattle, sheep and pigs by means of immunohistochemical techniques.
Using a polyclonal antibody against Orexin-A, the immunopositive reaction was identified in the cytoplasm of many insular cells in the three species studied.
Double immunohistochemical staining, using a polyclonal anti-insulin antibody, showed that Orexin-A is co-expressed with insulin.
Our results, besides showing the presence of Orexin-A in the endocrine pancreas of domestic animals,
together with data present in the literature, could contribute to the understanding of complex mechanisms regulating the functionality of the endocrine pancreas in domestic animals
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