977 research outputs found
Putative NAD(P)-binding Rossmann fold protein is involved in chitosan-induced peroxidase activity and lipoxygenase expression in Physcomitrella
No full textOxidative burst, the rapid production of high levels of reactive oxygen species in response to external stimuli, is an early defense reaction against pathogens. The fungal elicitor chitosan causes an oxidative burst in the moss Physcomitrium patens (formerly Physcomitrella patens), mainly due to the peroxidase enzyme Prx34. To better understand the chitosan responses in P. patens, we conducted a screen of part of a P. patens mutant collection to isolate plants with less peroxidase activity than wild-type (WT) plants after chitosan treatment. We isolated a P. patens mutant that affected the gene encoding NAD(P)-binding Rossmann fold protein (hereafter, Rossmann fold protein). Three Rossmann fold protein-knockout (KO) plants (named Rossmann fold KO lines) were generated and used to assess extracellular peroxidase activity and expression of defense-responsive genes, including alternative oxidase, lipoxygenase (LOX), NADPH oxidase, and peroxidase (Prx34) in response to chitosan treatment. Extracellular (apoplastic) peroxidase activity was significantly lower in Rossmann fold KO lines than in WT plants after chitosan treatments. Expression of the LOX gene in Rossmann fold KO plants was significantly lower before and after chitosan treatment when compared with WT. Peroxidase activity assays together with gene expression analyses suggest that the Rossmann fold protein might be an important component of the signaling pathway leading to oxidative burst and basal expression of the LOX gene in P. patens. [Graphic: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license
The geometrical and topological features of the canonical Rossmann β2-Asp/Glu motif.
No full text(A) Representative carboxylate-ribose bidentate interactions in Rossmann-fold enzymes. Structures were superpositioned by the ribose moiety of their cofactors. One complete backbone is shown (in cartoon), whilst for others, shown are the bound cofactor, the second β-strand (β2), and the interacting Glu or Asp. PDB (Protein Data Bank) IDs and corresponding cofactors: 1JG2, ADN; 3GVI, ADP; 2HMU, ATP; 2XXB, AMP; 1BWC, FAD; 1V5E, FAD; 1EG2, MTA; 2A14, 2PBF, 2AVD (complete structure), SAM; 2GR2, FAD; 1AHH, NAD; 1GEG, NAD; 1GZ6, NAI. (B) The distribution of the interaction angle (α) in structures of proteins with a ribose bound to an Asp/Glu via a bidentate interaction. α is defined by two vectors: v1, going through the CH2-COO- carbons of the interacting Asp/Glu side chain, and v2, going through the C1-C2 carbons of the ribose ring. Gray bars represent the angles in all Rossmann structures with the canonical motif (n = 263). Black bars represent the angles of all the noncanonical bidentate interactions found in both Rossmann and non-Rossmanns enzymes. The PDB Rossmann structures with canonical and noncanonical interaction and their α angles are listed in S1 Data. (C) Representative noncanonical bidentate interactions in non-Rossmann enzymes. PDBs and the corresponding cofactor: 1HO5, ADN; 2J9L (complete structure; the helix carrying the ribose-binding Asp727 is highlighted), ATP; 3S2U, UD1; 1K9Y, AMP; 2ATV, GDP; 1SIW, GDP; 3TE5, NAI; 1I7L, ATP; 4B45, GSP. These structures are shown individually in S6 Fig.</p
Gemeinsam aktiver? Der Einfluss von Gruppenbildern auf die körperliche Aktivität
No full textReifegerste D, Rossmann C. Gemeinsam aktiver? Der Einfluss von Gruppenbildern auf die körperliche Aktivität. In: Schäfer M, Quiring O, Rossmann C, Hastall MR, Baumann E, eds. Gesundheitskommunikation im gesellschaftlichen Wandel. Gesundheitskommunikation/Health Communication. Vol 10. Baden-Baden: Nomos; 2015: 179-188
Gewinn- und Verlustframing in der Gesundheitskommunikation
No full textWagner A. Gewinn- und Verlustframing in der Gesundheitskommunikation. In: Rossmann C, Hastall MR, eds. Handbuch der Gesundheitskommunikation. Wiesbaden: Springer VS; 2019: 517-526
Kommunikationswissenschaftliche Grundlagen der Gesundheitskommunikation
No full textRossmann C, Hastall MR, Baumann E. Kommunikationswissenschaftliche Grundlagen der Gesundheitskommunikation. In: Hurrelmann K, Baumann E, eds. Handbuch Gesundheitskommunikation. Bern: Hans Huber; 2014: 81-94
Selektion und Vermeidung von Gesundheitsbotschaften
No full textWagner A, Hastall MR. Selektion und Vermeidung von Gesundheitsbotschaften. In: Rossmann C, Hastall MR, eds. Handbuch der Gesundheitskommunikation. Wiesbaden: Springer VS; 2019: 221-232
Gesundheitskommunikation als Forschungsfeld der Kommunikations- und Medienwissenschaft
No full textBaumann E, Hastall MR, Rossmann C, Sowka A, eds. Gesundheitskommunikation als Forschungsfeld der Kommunikations- und Medienwissenschaft. Medien + Gesundheit. Baden-Baden: Nomos; 2014
Vielfalt und Herausforderungen der Evidenzbasierung in der strategischen Gesundheitskommunikation
No full textBaumann E, Reifegerste D. Vielfalt und Herausforderungen der Evidenzbasierung in der strategischen Gesundheitskommunikation. In: Stehr P, Heinemeier D, Rossmann C, eds. Evidenzbasierte | evidenzinformierte Gesundheitskommunikation. Gesundheitskommunikation/ Health Communication. Vol 19. Baden-Baden: Nomos Verlagsgesellschaft mbH & Co. KG; 2018: 73-84
Kranke, Psychopathen, Mörder? Mediale Repräsentationen von Erkrankungen und Erkrankten in TV-Krimiserien
No full textWagner A. Kranke, Psychopathen, Mörder? Mediale Repräsentationen von Erkrankungen und Erkrankten in TV-Krimiserien. In: Stehr P, Heinemeier D, Rossmann C, eds. Evidenzbasierte | evidenzinformierte Gesundheitskommunikation. Gesundheitskommunikation. Vol 19. Baden-Baden: Nomos; 2018: 219-230
Multielement (H, C, N, O, S) stable isotope characteristics of lamb meat from different Italian regions
No full textThe study focuses on the 2H/H, 13C/12C, 15N/14N, 18O/16O and 34S/32S values of defatted dry matter
(DFDM) and on the 2H/H, 13C/12C and 18O/16O values of the fat fraction of meat samples from various
lamb types reared in seven Italian regions, following different feeding regimes (forage, concentrate,
milk). The 13C/12C (r1⁄40.922), 2H/H (r1⁄40.577) and 18O/16O (r1⁄40.449) values of fat and DFDM are
significantly correlated, the fat values being significantly lower for C and H and higher for O than for
DFDM values and the differences between the two fractions not being constant for different lamb
types. The feeding regime significantly affected the 13C/12C, 15N/14N, 18O/16O and 2H/H of fat. The
DFDM 2H/H, and 18O/16O values, excluding an outlier, are significantly correlated with the corresponding
values in meteoric waters, thus allowing us to trace the variability of geoclimatic factors.
15N/14Nis influenced by pedoclimatic conditions, whereas 34S/32S is influenced by the sea spray effect
and the surface geology of the provenance area. By applying stepwise linear discriminant analysis
only the 2H/H of fat was found not to be significant and 97.7% of the samples were correctly assigned
to the lamb type and more than 90% cross-validated. With the feeding regime, 97.7% of the samples
were both correctly assigned and cross-validated using a predictive model including
13C/12C, 15N/14N, 18O/16O, 34S/32S of DFDM and 18O/16O of fat
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