1,721,073 research outputs found
Aspergillosi dell'apparato respiratorio degli uccelli
No full textAspergillosis is a common respiratory disease in birds. Potentially all species can be affected inlcuding poultry, aviary and pet birds, and wild birds. Aspergillus fumigatus is the most frequent isolated fungus. Birds are more susceptible to aspergillosis than mammalian species and their predisposition to aspergillosis is attributed to anatomical and physiological peculiarities, which prevent the ejection and removal of inhaled fungal spores from the respiratory tract. In this review we describe the epidemiology, pathogenesis, clinical features, and the commonest lesions of avian aspergillosis. Diagnosis, therapy, and control strategies are also discussed
Myotropic avian leukosis virus subgroup J infection in a chicken
No full textThe study describes a highly productive myotropic avian leukosis virus infection (ALV) in a 3-month-old female chicken. At necropsy, ascites, hepatic fibrosis and cardiomegaly were seen. Histologically, the most striking lesion was the presence of cytoplasmic basophilic inclusions in myocardial fibers. Immunostaining for ALV group specific antigen p27 revealed a diffuse presence of virus antigen in cardiac myofibers, in smooth muscle fibers of most of the organs, and in rare, pancreatic and ovarian theca cells. Ultrastructurally, myocardial inclusions consisted of clusters of 50-60 nm round particles with interspersed ribosome-like granules. Numerous C-type particles were found in intercellular spaces of ALV p27 positive tissues. PCR analyses revealed the presence of both ALV-E and ALV-J related sequences. In chicken genome, ALV-E is usually present as endogenous provirus therefore, the pathological findings observed in this case are considered to be related with the ALV-J infection. The results of this report further confirm that ALV-J may be responsible for highly productive myotropic infections
Association between clinico-pathological changes and type of lesions in toxicity studies on mice
No full textA simple method for the production of bacterial controls for immunohistochemistry and fluorescent in situ hybridization
No full textImmunohistochemical and fluorescent in situ hybridization (FISH) assays are useful diagnostic methods for the identification of bacteria on formalin fixed paraffin embedded histological sections. To validate an anti-bacterial antibody or an oligonucleotide probe and to ensure fidelity during subsequent analyses, suitable positive and negative controls are necessary. Suspensions of fixed bacteria are often used, but ideally, these controls should be fixed, embedded and processed in the same way of tissue samples under analysis. Herein, we describe a simple method for the production of bacterial histological control samples: the sandwich. The sandwich is composed of two external layers of equine lung parenchyma and a central layer of the target bacterium. We prepared sandwiches containing Escherichia coli, Campylobacter jejuni, and Arcanobacterium pyogenes and tested them with appropriate antibodies and Eub338 FISH probe. The sandwich is an effective and simple method to prepare bacterial histological controls fixed and processed in the same way as the diagnostic tissues
Circovirus inclusion bodies in intestinal muscle cells of a canary
No full textMultiple cytoplasmic inclusion bodies were observed in the intestinal smooth muscle cells of an adult canary from an aviary with a history of high mortality (50%) both in adult and young birds. Grossly, a mild enteritis was the only lesion appreciable. Smears of the proventricular contents contained a few megabacteria ( Macrorhabdus ornithogaster ). The intestinal inclusions were found in very high numbers in all parts of the tract examined. They appeared round to oval, amphophilic and hyaline in sections stained with haematoxylin and eosin, and magenta with Feulgen stain. Inclusions of the same type were occasionally detectable in the wall of a few splenic and pancreatic arteries. No inclusions or lesions were seen in the other organs examined. Transmission electron microscopy of the intestinal wall revealed circovirus-like particles either in paracrystalline arrays or loose arrangements, mostly within the cytoplasm of the intestinal muscule cells. Polymerase chain reaction amplification and sequence analysis confirmed infection with canary circovirus
- …
