525 research outputs found
Edoardo Robino: lo visible y lo invisible
No full textIn this paper we will demonstrate the originality of the work of Edoardo Robino, an Italian artist which thought can’t be considered without his artistic production: sculpture and writing must be considered as a unity, as two sides of the same path of interpretation of reality. In the same way, in the thought of Robino nature and culture are not separate, but represent the two faces of a process in which man is involved from the start. Identity is also difference, and therefore is not suitable to deny the unity of nature and culture. This unity in difference has been investigated by the author from a structural point of view and forma the contemporary Philosophy of language, producing a procedure that can be interesting for the contemporary Aesthetics.En este artículo pretendemos demostrar la originalidad de la obra de Edoardo Robino, artista italiano cuyo pensamiento no se puede separar de su producción artística: escultura y escritura tienen que considerarse en su unidad, como dos aspectos del mismo camino de interpretación de la realidad. En el mismo sentido, en el pensamiento de Robino, naturaleza y cultura no van separadas, sino que representan las dos caras de un proceso en que el hombre está implicado desde el principio. La identidad es también diferencia, y es por lo tanto irrazonable negar la unidad de naturaleza y cultura. Esta unidad en la diferencia ha sido estudiada por el autor a partir del Estructuralismo y de la filosofía del lenguaje contemporánea y ha dado lugar a un procedimiento del pensar que tiene mucho interés para la Teoría estética actual
L’Huile de Colza dans l’alimentation murine. Quelques considérations sur le métabolisme lipidique
No full textStaron Th., Moreau J.-P., Estève C., Robino F., Kollmann A., Lampaert R. L’Huile de Colza dans l’alimentation murine. Quelques considérations sur le métabolisme lipidique. In: Bulletin de l'Académie Vétérinaire de France tome 125 n°4, 1972. pp. 215-226
The Use of PowerPlex® Y23 System with the ABI PRISM® 310 Genetic Analyzer
No full textOver the last fifteen years, multiplex PCR typing of Y-chromosomal short tandem repeat (STR) loci has emerged as a powerful tool for forensic casework analysis, especially in sexual assault cases, where evidence typically consists of mixtures containing large amounts of female DNA in the presence of minor amounts of male DNA. However, since Y-STR loci are from nonrecombining portion of the Y chromosome, individuals from the same lineage typically share the same profile. Therefore, Y-STR profiles do not carry the same discrimination power as autosomal profiles. The availability of new multiplex PCR assays that allow simultaneous typing of conventional and additional Y-STRs improves the power of discrimination of paternal lineages and is therefore highly welcomed by the forensic community. Recently Promega launched the PowerPlex® Y23 System, which includes the Y-STR loci found in the AmpFlSTR® Yfiler® PCR Amplification Kit from Life Technologies plus six new loci that display high genetic diversity in human populations. Two of the loci, DYS570 and DYS576, are rapidly mutating loci (1) .
The PowerPlex® Y23 System Technical Manual TMD035 describes instrument setup and sample preparation of PCR products using the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. Here, we report the successful analysis of amplicons obtained with PowerPlex® Y23 on the ABI PRISM® 310 Genetic Analyzer. We performed sensitivity and mixture studies using control DNA samples and compared typing results in a limited set of casework samples previously typed with the Yfiler® kit
Test genetici di paternità in gravidanza : una scelta oppure un obbligo per l’operatore?
No full textThe rapid development of molecular diagnostic techniques in the last twenty years has
been followed by an ethical and regulatory adjustment which turned out to be difficult
and complex.
At the same time the media contribute to convey to the public the idea of a virtual absence
of limits, especially when dealing with genetic investigations, which often leads to an
unconscious violation of the same regulations. However it is not uncommon to observe
that these rules are sometimes violated with full consciousness.
This manuscript will analyse the issue of genetic paternity testing performed during
pregnancy in the light of the Italian legislation.
This study does not cover a full revision of the international legislation, but it could
become a starting point in order to discuss an issue which is currently credited with only
marginal consideration.
After reviewing the main analytical techniques and the legal status of the embryo, the
Authors revised the legal framework and the principles of the medical code of conduct
regarding genetic discrimination in relation to the Law n. 194/1978 (the Italian law
governing the voluntary termination of pregnancy in some specific situations).
The main point to consider is whether DNA paternity tests are legally allowed during
pregnancy.
In either case there are significant legal and ethical implications for the health care
professional when asked for prenatal paternity testing.
From this study emerges the need for a legal clarification aimed at standardising the
rules, avoiding arbitrary decisions
Applicazione di test immunocromatografici per l’identificazione della naura di tracce biologiche
No full textDevelopment of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample
No full textTwo multiplex polymerase chain reaction systems for the automated profiling of 12 X-chromosomal short tandem repeat (STR) markers were developed. Multiplex A consisted of DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, and DXS8377. Multiplex B consisted of DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, and DXS10011. The set of amplified X-STRs was designed to include groups of closely linked markers (DXS101–DXS7424 and DXS6789–DXS6801–DXS6809) to generate highly informative haplotypes for kinship testing. A population genetics study of the 12 X-STRs was conducted in a northwestern Italian population sample (n=160, 80 women and 80 men). A diallelic pattern at locus DXS6789 was observed in one man
Subtyping of Y-chromosomal haplogroup E-M78 (E1b1b1a) by SNP assay and its forensic application.
No full textThe continual discovery of new single-nucleotide polymorphisms (SNPs) has led to an increased resolution of the Y chromosome phylogeny. Some of these Y-SNPs have shown to be restricted to small geographical regions and therefore may prove useful in the forensic field as tools for the prediction of population of origin of unknown casework samples. Here, we describe a system for the molecular dissection of haplogroup E-M78 (E1b1b1a), consisting of multiplex polymerase chain reaction and minisequencing of M78 and nine population-informative Y-SNPs (M148, M224, V12, V13, V19, V22, V27, V32, V65) in a single reaction. Sensitivity and admixture studies demonstrated that the SNP protocol allows robust genotyping from as little as 50 pg of male DNA, even in the presence of 500-fold amounts of female DNA. In order to evaluate the suitability of E1b1b1a, subhaplogrouping for population-of-origin prediction, the distribution of E-M78 and its derived variants was determined in an Italian population sample (n = 326)
Integration of the AmpFlSTR Identifiler PCR Amplification Kit with SRY-specific primers for gender identification
No full textDropout of the amelogenin Y-specific allele due to an interstitial deletion of the Yp involving the amelogenin Y locus (AMELY) can cause misidentification of sex genotype with potentially serious consequences in personal identification processes and criminal investigations. Inclusion of additional sex-defining markers in forensic DNA typing kits is therefore advisable. In this study, the co-amplification of the sex-determining region Y (SRY) gene and 16 STR loci included in the AmpFlSTR Identifiler PCR Amplification Kit was evaluated. Combination of SRY and Identifiler primers did not compromise the amplification outcome and generated a 90 bp male-specific SRY fragment, showing a reproducible peak height ratio in comparison with the AMELY peak. The SRY peak was detectable in presence of amounts of template DNA as low as 125 pg, and in mixed samples with a male/female DNA ratio of 1:100
HUMARA X-chromosome inactivation assay for detection of female minor component in male/female mixed bloodstains
No full textAnalysis of mixed stains from forensic casework by means of standard PCR-based typing of autosomal short tandem repeats (STRs) becomes difficult when the minor component is present at less than one tenth of the concentration of the major component. The human androgen receptor (HUMARA) X-chromosome inactivation assay allows to detect even a small number of female cells in the presence of a high background of male cells in male/female mixed bloodstains. DNA cleavage by means of methyl-sensitive restriction enzyme (HhaI) is followed by typing of the HUMARA locus with the nested PCR technique: methylation of restriction sites on inactivated female X chromosomes allows the efficient amplification of low amounts of endonuclease-resistant female-derived target sequences. In this study, the method attained a 10−3 level of sensitivity in the detection of the female minor component
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