1,721,036 research outputs found
Prevalence of feline bartonellosis and multilocus sequence typing of Bartonella henselae isolates in urban stray cats living in Milan, Italy
No full textCat scratch disease is a worldwide zoonosis caused predominatly by Bartonella henselae and in a lesser extent by B. clarridgeiae. Cats are the natural reservoir and vectors for B. henselae and B. clarridgeiae infections in humans. Genetic heterogeneity of B. henselae strains has been reported and multiple sequence types (STs) have been identified by the use of multilocus sequence typing (MLST). Particular sequence types have been more frequently associated with zoonosis than others. The aim of this study was to evaluate the prevalence of B. henselae and B. clarridgeiae infection in stray cats from Milan, Italy and to explore the genotypes of the B. henselae population for the evaluation of the potential risk of transmission to humans. Whole blood samples collected from 89 stray cats were cultured and analyzed by PCR. Sequence types of the feline B. henselae isolates were delineated using MLST. Bartonella henselae was detected in four (4.5%) cats and B. clarridgeiae was detected in one (1.1%) cat by PCR on blood samples. Coinfection by B. henselae type I and type II was identified in one cat. Four B. henselae isolates were cultured and were characterized as ST1 (2/4), ST5 (1/4) and ST8 (1/4), that are more commonly regarded as human associated or zoonosis associated STs. Typical feline associated B. henselae STs were not observed. Despite the low prevalence of B. henselae infection in stray cats from Milan, further investigation are needed to assess the risk for human health
Diarrea neonatale bovina : Escherichia coli e il fenomeno dell’antibiotico-resistenza
No full textEscherichia coli is a normal inhabitant of the gastrointestinal tract of humans and animals. Several highly adapted clones have acquired specific virulence characters and have become pathogenic. Pathotypes of E.coli represent the main cause of health problems and economical losses in calves. In the last years E. coli strains resistant to multiple drugs have been found, especially in calves affected by neonatal diarrhoea. Aim of the work was to evaluate the trend of antibiotic resistance of some E. coli strains isolated from calves. Results show a significant prevalence of multi antibiotic resistance in E. coli strains. Furthermore, the increasing level of resistance to antimicrobial agents important in treating human diseases, such as cephalospotins and fluoroquinolones, may represent a significant public health concern
Evaluation of molecules with antiprionic activity : inhibition of PrP deposition in mouse neuroblastoma cells
No full textEncefalopatie spongiformi trasmissibili: valutazione in vitro mediante HPLC, dell’attività antifibrillogenica di potenziali molecole terapeutiche nei confronti del peptide sintetico 106-126
No full textSusceptibility profiles of Streptococci isolated from bovine milk samples in Italy
No full textBovine mastitis is the most economically important disease in dairy herds. Intramammary infections are caused by bacterial pathogens. Streptococci are frequently isolated from bovine mastitis in dairy farms, and they are generally very susceptible to β-lactam antibiotics. For that reason, susceptibility tests are usually not requested by veterinarians. We report susceptibility profiles observed in Streptococci isolated from milk samples from 12 different dairy herds in Lombardy region, Italy. We tested overall 25 strains: 7 of S. agalactiae, 1 of S. dysgalactiae, 6 of S. uberis, 3 of Streptococcus ssp. and 8 of Enterococcus faecalis. The antimicrobial susceptibility was determined using the disk diffusion test. We found resistance to penicillin in 52% of isolates, to ampicillin in 60%, to amoxicillin-clavulanic acid in 8%, to 1st generation cefalosporines in 36%, to 2nd generation cefalosporines in 12,5%, to 3rd generation cefalosporines in 40%, to 4th generation cefalosporines in 48%, to oxacillin in 80%, to nafcillin-penicillin-streptomicin in 16%, to tylosin in 41,6%, to erythromycin in 50%, to rifaximin in 36%, to norfloxacin in 65,2%, to tiamfenicol in 18,1%, and to kanamicin in 100%. Particularly, susceptibility profiles of S. agalactiae were surprising: 71,4% of the isolates showed resistance to penicillin, 85,7% to ampicillin, 14,2% to amoxicillin-clavulanic acid, 71,4% to 1st generation cefalosporines, 25% to 2nd generation cefalosporines, 42,8% to 3rd generation cefalosporines, 71,4% to 4th generation cefalosporines, 100% to oxacillin, 71,4% to nafcillin-penicillin-streptomicin, 57,1% to tylosin, 57,1% to erythromycin, 57,1% to rifaximin, 100% to norfloxacin, 40% to tiamfenicol and 100% to kanamicin. These results are quite different from those reported in different studies in our country. We believe this is the first report of extended resistance to β-lactam drugs of S. agalactiae isolated from milk samples in Italy. Therefore, frequent monitoring of Streptococci antibiotic susceptibility becomes a central point in the diagnosis of mastitis, in order to verify the spread of resistant strains among herds
Development of in vitro cell cultures for the evaluation of molecules with antiprionic activity
No full textDetermination of 5-chloro-7-iodo-8-quinolinol (clioquinol) in plasma and tissues of hamsters by high-performance liquid chromatography and electrochemical detection
No full textThis paper describes a method of determining clioquinol levels in hamster plasma and tissue by means of HPLC and electrochemical detection. Clioquinol was separated on a Nucleosil C18 300 x 3.9 mm I.D. 7 μm column at 1 ml/min using a phosphate/citrate buffer 0.1 M (400 ml) with 600 ml of a methanol:acetonitrile (1:1, v/v) mobile phase. The retention times of clioquinol and the IS were respectively 11.6 and 8.1 min; the quantitation limit (CV>8%) was 5 ng/ml in plasma and 10 ng/ml in tissues. The intra- and inter-assay accuracies of the method were more than 95%, with coefficients of variation between 3.0% to 7.7%, and plasma and tissue recovery rates of 72-77%. There was a linear response to clioquinol 5-2000 ng/ml in plasma, and 10-1000 ng/g in tissues. The method is highly sensitive and selective, makes it possible to study the pharmacokinetics of plasma clioquinol after oral administration and the distribution of clioquinol in tissues, and could be used to monitor plasma clioquinol levels in humans
Microarray per l'identificazione simultanea di Equid herpesvirus, West Nile virus e Borna disease virus in equini
No full textIn vitro evaluation of molecules with antiprionic activity : inhibition of PrP deposition in mouse neuroblastoma cells and interaction with the synthetic peptide 106-126
No full textValutazione dell’attività antiprionica del cliochinolo in modelli cellulari e sistemi cell-free
No full text- …
