302 research outputs found
Akinetic Tuneable Optical Sources with Applications
Get PDFOptical Coherence Tomography (OCT) is a modern, non-invasive imaging technique in biomedical research and medical diagnostics. It was initially developed for clinical applications in ophthalmology, providing high-resolution, cross-sectional images of the retina, retinal nerve fibre layer and the optic nerve head. Today, OCT is used for in vivo imaging of almost every type of tissue and it also branched out in fields outside medicine, like industrial or pharmaceutical applications.
OCT is a continuously improving imaging technique, benefiting from the development of advanced optical components and broadband optical sources. The objective of the work presented in the thesis was the development of both short and, respectively, long cavity akinetic optical devices, employing several types of dispersive optical fibre components in the cavity, like chirped fibre Bragg gratings, single mode or dispersion compensating fibre, and actively radio-frequency tuned semiconductor optical amplifiers, used as gain media. The use of external modulators, like Fabry-Perot assemblies, rotating mirrors and other mechanical devices is therefore completely eliminated, while versatility is added in the control of the coherence length, output bandwidth, repetition rate and power.
The short cavity source was developed in the 1060 nm region, the output power and bandwidth showing a slow decay with the increase of repetition rate up to 250 kHz. Without any booster, the power achieved was 2 mW at 100 kHz.
A novel dual-mode-locking mechanism was developed in order to tune an akinetic swept source based on dispersive cavities at a repetition rate close to, but different from the inverse of the cavity roundtrip. Several optical source configurations emitting in the 1060 nm or 1550 nm wavelength region were developed, characterised and tested in OCT applications. For the 1550 nm swept source employing a Faraday Rotating Mirror in a dispersive cavity, sweeping rates in the range of MHz were achieved, from 782 kHz to up to 5 times this value, with proportional decrease in the tuning bandwidth. Linewidths smaller than 60 pm and output powers exceeding 10 mW were measured. OCT topographic imaging was demonstrated.
The thesis ends with a proposed broadband investigation of microresonators written in silica glass employing akinetic optical sources at 1550 nm.
The work presented in this thesis resulted in several peer reviewed papers, one patent application and several conference presentations, listed after the final conclusions
Optical coherence tomography in archaeological and conservation science - a new emerging field
Get PDFThere has been a long tradition of applying biomedical imaging techniques to the examination of historical artefacts, owing to similar demands for non-invasive methods in both fields. Optical Coherence Tomography (OCT) is no exception. We review the achievements on OCT applications to art conservation and archaeology since the publication of the first papers in 2004. Historical artefacts include a much broader range of materials than biological tissues, hence presenting a greater and somewhat different challenge to the field of OCT. New results will be presented to illustrate the various applications of OCT including both qualitative and quantitative analysis
Surface imaging of metallic material fractures using optical coherence tomography
Get PDFWe demonstrate the capability of optical coherence tomography (OCT) to perform topography of metallic surfaces after being subjected to ductile or brittle fracturing. Two steel samples, OL 37 and OL 52, and an antifriction Sn-Sb-Cu alloy were analyzed. Using an in-house-built swept source OCT system, height profiles were generated for the surfaces of the two samples. Based on such profiles, it can be concluded that the first two samples were subjected to ductile fracture, while the third one was subjected to brittle fracture. The OCT potential for assessing the surface state of materials after fracture was evaluated by comparing OCT images with images generated using an established method for such investigations, scanning electron microscopy (SEM). Analysis of cause of fracture is essential in response to damage of machinery parts during various accidents. Currently the analysis is performed using SEM, on samples removed from the metallic parts, while OCT would allow in situ imaging using mobile units. To the best of our knowledge, this is the first time that the OCT capability to replace SEM has been demonstrated. SEM is a more costly and time-consuming method to use in the investigation of surfaces of microstructures of metallic materials. © 2014 Optical Society of America
Evaluation of Tizian overlays by means of a swept source optical coherence tomography system
No full textThe teeth affected by pathologic attrition can be restored by a minimally invasive approach, using Tizian overlays. In this study we prove the advantages of a fast swept source (SS) OCT system in the evaluation of Tizian overlays placed in an environment characterized by high occlusal forces. 12 maxillary first premolars were extracted and prepared for overlays. The Tizian overlays were subjected to 3000 alternating cycles of thermo-cycling (from -10°C to +50°C) and to mechanical occlusal overloads (at 800 N). A fast SS OCT system was used to evaluate the Tizian overlays before and after the mechanical and thermal straining. The SS (Axsun Technologies, Billerica, MA) has a central wavelength of 1060 nm, sweeping range of 106 nm (quoted at 10 dB) and a 100 kHz line rate. The depth resolution of the system, measured experimentally in air was 10 ?m. The imaging system used for this study offers high spatial resolutions in both directions, transversal and longitudinal of around 10 ?m, a high sensitivity, and it is also able to acquire entire tridimensional (3D)/volume reconstructions as fast as 2.5 s. Once the full dataset was acquired, rendered high resolutions en-face projections could be produced. Using them, the overlay (i.e., cement) abutment tooth interfaces were remarked both on B-scans/two-dimensional (2D) sections and in the 3D reconstructions. Using the system several open interfaces were possible to detect. The fast SS OCT system thus proves useful in the evaluation of zirconia reinforced composite overlays, placed in an environment characterized by high occlusal forces. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only
OCT evaluation of single ceramic crowns: comparison between conventional and chair-side CAD/CAM technologies
No full textDigital impressions were introduced to overcome some of the obstacles due to traditional impression materials and techniques. The aim of this in vitro study is to compare the accuracy of all ceramic crowns obtained with digital impression and CAD-CAM technology with the accuracy of those obtained with conventional impression techniques. Two groups of 10 crowns each have been considered. The digital data obtained from Group 1 have been processed and the all-ceramic crowns were milled with a CAD/CAM technology (CEREC MCX, Sirona). The all ceramic crowns in Group 2 were obtained with the classical technique of pressing (emax, Ivoclar, Vivadent). The evaluation of the marginal adaptation was performed with Time Domain Optical Coherence Tomography (TD OCT), working at a wavelength of 1300 nm. Tri-dimensional (3D) reconstructions of the selected areas were obtained. Based on the findings in this study, one may conclude that the marginal accuracy of all ceramic crowns fabricated with digital impression and the CAD/CAM technique is superior to the conventional impression technique. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only
Roughness measurements using optical coherence tomography: a preliminary study
Get PDFTo determine the roughness is an important aspect in both industrial and biomedical applications. We propose and utilize for roughness evaluations, a non-destructive evaluation methods, Optical Coherence Tomography (OCT). For the metallic surfaces investigated from this point of view, the Ra and Rz parameters are utilized, according to ISO 4287/1988. Also, according to ISO 4280 and ISO 3274 standards, the measurements have been made on 12.5 mm portions. In order to accommodate such evaluations with the specific OCT field-of-view, four consecutive OCT images have been made for each sample, and an appropriate processing of the data collected from the surface profiles has been made. A validation of the results obtained with OCT has been completed with the gold standard for such evaluations, using a contact mechanical method, with a Mitutuyo profiler
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Non-invasive imaging of subsurface paint layers with optical coherence tomography
No full textOptical coherence tomography (OCT) systems are fast scanning infrared Michelson interferometers designed for the non-invasive examination of the interiors of the eye and subsurface structures of biological tissues. OCT has recently been applied to the non-invasive examinations of the stratigraphy of paintings and museum artefacts. So far this is the only technique capable of imaging non-invasively the subsurface structure of paintings and painted objects. Unlike the traditional method of paint cross-section examination where sampling is required, the non-invasive and non-contact nature of the technique enables the examination of the paint cross-section anywhere on a painting, as there is no longer an issue with conservation ethics regarding the taking of samples from historical artefacts. A range of applications of the technique including the imaging of stratigraphy of paintings and painted artefacts, the imaging of underdrawings to the analysis of the optical properties of paint and varnish layers is presented. Future projects on the application of OCT to art conservation are discussed
Swept source optical coherence tomography Gabor fusion splicing technique for microscopy of thick samples using a deformable mirror
Get PDFWe present a swept source optical coherence tomography (OCT) system at 1060 nm equipped with a wavefront sensor at 830 nm and a deformable mirror in a closed-loop adaptive optics (AO) system. Due to the AO correction, the confocal profile of the interface optics becomes narrower than the OCT axial range, restricting the part of the B-scan (cross section) with good contrast. By actuating on the deformable mirror, the depth of the focus is changed and the system is used to demonstrate Gabor filtering in order to produce B-scan OCT images with enhanced sensitivity throughout the axial range from a Drosophila larvae. The focus adjustment is achieved by manipulating the curvature of the deformable mirror between two user-defined limits. Particularities of controlling the focus for Gabor filtering using the deformable mirror are presented. © 2015 Society of Photo-Optical Instrumentation Engineers
Master slave en-face OCT/SLO
Get PDFMaster Slave optical coherence tomography (MS-OCT) is an OCT method that does not require resampling of data and can be used to deliver en-face images from several depths simultaneously. As the MS-OCT method requires important computational resources, the number of multiple depth en-face images that can be produced in real-time is limited. Here, we demonstrate progress in taking advantage of the parallel processing feature of the MS-OCT technology. Harnessing the capabilities of graphics processing units (GPU)s, information from 384 depth positions is acquired in one raster with real time display of up to 40 en-face OCT images. These exhibit comparable resolution and sensitivity to the images produced using the conventional Fourier domain based method. The GPU facilitates versatile real time selection of parameters, such as the depth positions of the 40 images out of the set of 384 depth locations, as well as their axial resolution. In each updated displayed frame, in parallel with the 40 en-face OCT images, a scanning laser ophthalmoscopy (SLO) lookalike image is presented together with two B-scan OCT images oriented along rectangular directions. The thickness of the SLO lookalike image is dynamically determined by the choice of number of en-face OCT images displayed in the frame and the choice of differential axial distance between them
Handheld scanning probes for Optical Coherence Tomography: Developments, applications and perspectives
No full textWe present the handheld scanning probes that we have recently developed in our current project for biomedical imaging in general and for Optical Coherence Tomography (OCT) in particular. OCT is an established, but dynamic imagistic technique based on laser interferometry, which offers micrometer resolutions and millimeters penetration depths. With regard to existing devices, the newly developed handheld probes are simple, light and relatively low cost. Their design is described in detail to allow for the reproduction in any lab, including for educational purposes. Two probes are constructed almost entirely from off-the-shelf components, while a third, final variant is constructed with dedicated components, in an ergonomic design. The handheld probes have uni-dimensional (1D) galvanometer scanners therefore they achieve transversal sections through the biological sample investigated - in contrast to handheld probes equipped with bi-dimensional (2D) scanners that can also achieve volumetric (3D) reconstructions of the samples. These latter handheld probes are therefore also discussed, as well as the possibility to equip them with galvanometer 2D scanners or with Risley prisms. For galvanometer scanners the optimal scanning functions studied in a series of previous works are pointed out; these functions offer a higher temporal efficiency/duty cycle of the scanning process, as well as artifact-free OCT images. The testing of the handheld scanning probes in dental applications is presented, for metal ceramic prosthesis and for teeth. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only
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