11,857 research outputs found
Guidebook for Pre-conference North Island Field Trip A1 ‘Ashes to Issues’, 28-30 November, 2008
No full textWelcome to New Zealand or Aotearoa – „Land of the long lingering day [twilight]‟ – and to our three-day pre-conference North Island field trip „Ashes and Issues‟. We trust your stay in New Zealand is both informative and friendly and there is something for everyone on the trip. The itinerary in brief and a map of the North Island showing the main scientific stops are shown above. At the time of guidebook preparation, we have a group of 23, including four students, on the tour with participants from Japan, Taiwan, USA, UK, Australia and New Zealand. The tour leaders are Prof David Lowe (Univ. of Waikato, Hamilton) and Dr Haydon Jones (Scion Research, Rotorua). Assistant leader is Prof Paul McDaniel (Univ. of Idaho, Moscow), on leave at the Univ. of Waikato July-December, 2008. We offer a warm welcome to you all. Because we have considerable distances to travel (especially Day 3), as well as a range of stops planned, we will need to leave the hotel at 8.00 am each day
Study of the cell biological role of Lowe Syndrome protein OCRL1
No full textOculocerebrorenal syndrome of Lowe (OCRL) is caused by mutations in a
phosphatidylinositol 5-phosphatase, OCRL1, and is believed to lead to an
elevation of its preferred substrate, PI(4,5)P2. To date, much of the work on
OCRL1 has centred on its role at Golgi and endosomal membranes.
However, there is also evidence of plasma membrane activity for OCRL1,
where its PI(4,5)P2 substrate is known to be highly abundant. PI(4,5)P2
regulates a wide array of downstream cellular functions such as cytoskeletal
dynamics, membrane trafficking and signalling. The tight regulation of
PI(4,5)P2 levels and localisation, like other phosphoinositides, provides a
framework upon which many of these cellular processes work. In this thesis,
effects of OCRL1 loss have been tested through siRNA depletion of OCRL1,
focussing where possible on multiple PI(4,5)P2-dependent mechanisms, and
also focussing on cells forming polarised epithelia. Firstly, we have visualised
the localisation of PI(4,5)P2 in living HeLa cells lacking OCRL1 through
immunostaining for Annexin A2, which showed a marked translocation to the
plasma membrane. This change in distribution of Annexin A2 suggested that
OCRL1 depletion may have an effect on intracellular calcium dynamics as
well as PI(4,5)P2 localisation. We also used a GFP-chimera of the well
characterised PI(4,5)P2-binding pleckstrin homology domain of PLCδ1. This
showed no difference in localisation upon OCRL1 depletion. As OCRL1 is
highly enriched at the TGN, we fused the pleckstrin homology domain of
PLCδ1 to a mutated pleckstrin homology domain of OSBP known to bind ARF1 at the TGN, to act as a coincidence detector for PI(4,5)P2 at the TGN.
This construct also showed no reproducible effect of OCRL1 depletion.
Secondly we tested the effect of loss of OCRL1 on cytosolic calcium levels.
Using two phospholipase C (PLC) agonists, and a SERCA pump inhibitor, we
found no consistent differences in calcium handling upon depletion of OCRL1.
Thirdly, we have assessed the potential specialised role that OCRL1 has in
polarised epithelial cells, which might relate to the clinical picture in Lowe
Syndrome. We found that OCRL1 targets the tight junctions of immortalised
lines and primary cells. Through co-immunoprecipitation, we found OCRL1 in
complexes with the tight junction scaffold protein ZO-1. Most significantly, we
found that depletion of OCRL1 in human polarised epithelial cell lines
interfered with epithelial differentiation, reducing cell number and altering
morphology, to produce large flat cells. We attribute this phenotype, stronger
than any other so far described experimentally, to a defect in tight junction
maturation
Lifecourse social position and D-dimer; findings from the 1958 British birth cohort
No full textThe aim is to examine the association of lifecourse socioeconomic position (SEP) on circulating levels of D-dimer. Data from the 1958 British birth cohort were used, social class was determined at three stages of respondents' life: at birth, at 23 and at 42 years. A cumulative indicator score of SEP (CIS) was calculated ranging from 0 (always in the highest social class) to 9 (always in the lowest social class). In men and women, associations were observed between CIS and D-dimer (P<0.05). Thus, the respondents in more disadvantaged social classes had elevated levels of D-dimer compared to respondents in less disadvantaged social class. In multivariate analyses, the association of disadvantaged social position with D-dimer was largely explained by fibrinogen, C-reactive protein and von Willebrand Factor in women, and additionally by smoking, alcohol consumption and physical activity in men. Socioeconomic circumstances across the lifecourse at various stages also contribute independently to raised levels of D-dimer in middle age in women only. Risk exposure related to SEP accumulates across life and contributes to raised levels of D-dimer. The association of haemostatic markers and social differences in health may be mediated by inflammatory and other markers
Role of tephra in dating Polynesian settlement and impact, New Zealand
No full textTephrochronology in its original sense is the use of tephra layers as time-stratigraphic marker beds to establish numerical or relative ages (Lowe and Hunt, 2001). Tephra layers have been described and studied in New Zealand for more than 160 years (the German naturalist and surgeon Ernst Dieffenbach described ‘recognizable’ tephra sections in his 1843 book Travels in New Zealand), and the first isopach map, showing fallout from the deadly plinian basaltic eruption of Mt Tarawera on 10 June 1886, was published in 1888 (Lowe, 1990; Lowe et al., 2002). More recently, a wide range of tephra-related paleoenvironmental research has been undertaken (e.g., Lowe and Newnham, 1999; Newnham and Lowe, 1999; Newnham et al., 1999, 2004; Shane, 2000), including new advances in the role of tephra in linking and dating sites containing evidence for abrupt climatic change (e.g., Newnham and Lowe, 2000; Newnham et al., 2003). Here we focus on the use of tephrochronology in dating the arrival and impacts of the first humans in New Zealand, a difficult problem for which this technique has proven to be of critical importance
R. C. Seaton. Napoléon et sir Hudson Lowe, traduit de l'anglais par P. Guye, 1909
No full textGonnard Philippe. R. C. Seaton. Napoléon et sir Hudson Lowe, traduit de l'anglais par P. Guye, 1909. In: Revue d'histoire moderne et contemporaine, tome 13 N°2,1909. pp. 232-234
Barbara C. Lowe.
No full textR-P of B. Lowe. 7 Feb. SR 1620,55-3, vl, 2p. [3739] or HR 1478,55-2, v6, 2p. [3722] Service against Indians on the Texas frontier
Barbaracurus somalicus Kovařík & Lowe & Šťáhlavský 2018, comb. n.
No full text<i>Barbaracurus somalicus</i> (Hirst, 1907) comb. n. <p>(Figures 3, 27, 34, 54–57, 74–75, 83–84, 113–146, 263–265, Table 1)</p> <p> <i>Babycurus somalicus</i> Hirst, 1907: 208; Lamoral & Reynders, 1975: 498; Kovařík, 1998: 104; Fet & Lowe, 2000: 79; Kovařík, 2000: 255–256, figs. 10, 21, tables 1–3; Kovařík, 2003: 134.</p> <p>TYPE LOCALITY AND TYPE DEPOSITORY. Somaliland, Berbera and Durbar; BMNH.</p> <p> MATERIAL EXAMINED. <b>Somaliland</b>, Berbera and Durbar, 400 ft., leg. G. W. Bury, 1♀ (holotype), BMNH; Borama, campus Amound University, 09°56'49"N 43° 13'23"E, 1394 m a.s.l., 4-5.II.2017 (locality No. <b>17 SA</b>), 1♀, 9.-13.IX.2017, (locality No. <b>17SR</b>, Fig. 145), 4♂ 3♀ 4juvs. (Figs. 27, 35, 113–144, 260–261, Nos. 1308, 1309, 1332), leg. F. Kovařík, T. Mazuch & P. Just, FKCP.</p> <p>DIAGNOSIS. Total length of adult males 32–36 mm, adult female 38–47 mm. Coloration yellowish brown to grey with darker markings, chelicerae yellow without or with traces of reticulation. Pedipalp chela manus much wider in male than female, chela length/width ratio 3.45 in male and 4.15 in female; proximal margins of pedipalp fingers of female almost straight (Fig. 57, 135), of male strongly undulate so as to leave a gap with fingers closed (Figs. 55, 129); dentate margin of movable finger armed with 6 rows of granules, and a short apical row of 4 denticles (Fig. 3); most proximal granule row with one external accessory granule. Pectines with 17–20 teeth in both sexes. Hemispermatophore basal lobe a weak carina (Figs. 27, 35). No sexual dimorphism in length and width of metasomal segments (Figs. 74–75); metasoma I with 10 carinae, II–IV with 8 carinae. Telson setose, bearing numerous long macrosetae and short, pointed subaculear tubercle; vesicle smooth, elongate, pyriform, telson length/depth ratio 2.75–2.89 in both sexes; aculeus slender, curved, shorter than vesicle.</p> <p> NOTE. Until now, only the holotype and paratype females were known. The recent collection of both sexes of <i>B. somalicus</i> by one of us (F.K.) enables us to show photographs of live specimens, especially of the male, for the first time, and to characterize their sexual dimorphism.</p> <p> COMMENTS ON LOCALITY AND LIFE STRATEGY. The first author (F.K.) visited the locality 17SA on 4–5 February 2017 (winter dry season). At this locality, the author recorded a daytime temperature of 24.7 ºC (4 February, 16:08 h), and nighttime temperatures of 21.4 ºC shortly after sunset, dropping to 19.3 ºC (minimum temperature on 5 February at 19:20 h). The recorded humidity was 41% on 5 February at 19:20 h. The first author (F.K.) again visited the same locality on 9–13 September 2017 (summer minor dry season, 17SR) and recorded maximum daytime temperatures of 29.1 ºC (10th September 2017) and 31.8 ºC (12 September 2017), and a minimum nighttime temperature of 19.6 ºC. The recorded humidity was between 31% (minimum at night) and 79% (maximum at day). All specimens were collected at night by ultraviolet (UV) detection near rocks. At this locality, in addition to <i>B. somalicus</i>, the first author also recorded <i>Neobuthus</i> sp., <i>Parabuthus abyssinicus</i> Pocock, 1901 (Buthidae) and <i>Pandinurus kmoniceki</i> Kovařík et al., 2017 (type locality) (Scorpionidae). Fifty metres from this rocky site is a riverbed of an occasional river (figs. 45–48 in Kovařík et al., 2017: 18) where in addition the author recorded <i>Gint amoudensis</i> Kovařík et al., 2018 (type locality) (Buthidae), and <i>Pandinops pugilator</i> (Pocock, 1900) (Scorpionidae).</p>Published as part of <i>Kovařík, František, Lowe, Graeme & Šťáhlavský, František, 2018, Review of the genus Babycurus Karsch, 1886 (Arachnida, Scorpiones, Buthidae), with descriptions of Barbaracurus gen. n. and two new species from Oman and Yemen, pp. 1-41 in Euscorpius 267</i> on pages 25-27, DOI: <a href="http://zenodo.org/record/6544157">10.5281/zenodo.6544157</a>
Neobuthus awashensis Kovarik et Lowe 2012
No full textNeobuthus awashensis Kovařík et Lowe, 2012 (Figs. 1–6, 158, 161–165) Neobuthus awashensis Kovařík & Lowe, 2012: 7–16, figs. 5–6, 18–21, 34–38, 44–47, 67–74, 86, 89, 92, 95–96, 100–101; Kovařík et al., 2015: 30. TYPE LOCALITY AND TYPE REPOSITORY. Ethiopia, Awash, Metahara env., 08°54' N 39°54' E, 960-1050 m a.s.l., FKCP. TYPE MATERIAL. Ethiopia, Awash, Metahara env., 08°54' N 39°54' E, 960-1050 m a.s.l., 1♀ (paratype), 2008, leg. V. Trailin, 2♀1♀ im. (allotype and paratypes), XI.2010, leg. T. Mazuch and P. Novák, 32♂ (holotype and paratypes) 18♀ (paratypes) 11♀ ims, 5♂ ims (paratypes), 19.-22.VII.2011, leg. F. Kovařík. Most types are in the collection of the second author (FKCP), two paratypes (♂ ♀) are in the collection of the first author (GL). OTHER MATERIAL EXAMINED. Ethiopia, 11°43'22" N 40° 56'52" E, 457 m a.s.l. (Locality No. 12 EMA), 20.XI. 2012, 1♀1♀ im., leg. F. Kovařík (UV detection), FKCP; 11°43'30" N 40°58'45" E, 404 m a.s.l. (Locality No. 12EM), 20.XI.2012, 1♂, leg. F. Kovařík (UV detection), FKCP; Gewane, 10°09'38" N 40°39'45" E, 631 m a.s.l. (Locality No. 12 EO), 23.XI.2012, 1♂ 1♀, leg. F. Kovařík, (UV detection), FKCP; 09°08'10.4" N 40°09' 45.5" E, 835 m a.s.l. (Locality No. 12ER), 24.XI.2012, 12♂ 1♀ 1juv., leg. F. Kovařík (UV detection), FKCP, 26.-27.XI.2014, 8♂ 2♀ 2juvs, FKCP, 3♂ 2juvs, GL, leg. F. Kovařík; Awash, Metahara env., 08°54' N 39°54' E, 960-1050 m a.s.l. (Locality No. 12 EX), 25.XI.2012, 7♂ 6♀ 5juvs., 27.-30.XI.2014, 7♂ 1♀, topotypes, leg. F. Kovařík (UV detection), FKCP. EMENDED DIAGNOSIS. Total length 18–22 mm (males), 22.5–30 mm (females); carapace with area between anterior median carinae fuscous; tergites with fuscous pigmentation unbroken across median area; pedipalp relatively slender, males with femur L/ W 2.50 –2.70, patella L/ W 2.45 –2.70, chela L/ W 4.63 –5.08; chela movable finger with 5–6 subrows of primary denticles, 3–5 external accessory denticles flanking proximal end of each subrow; trichobothria d 2 usually absent from femur and patella; posterior margins of carapace and tergites usually bearing 2–4 macrosetae; pedipalps, legs, metasoma and telson with short, stout macrosetae in males, and long, fine setae in females; males with coxae sparsely granulated, sternites III–VI lightly shagreened to smooth, sternite VII finely granulated with 4 weak, granulated carinae; females with sternites III–VI smooth, sternite VII sparsely shagreened with 4 weak carinae, median carinae granulated; metasoma I–III with median lateral carinae present in both sexes; lateral surface of metasoma V in males densely granulated, with granules separated; soles of telotarsi with relatively sparse setation, leg III of adults with 6–9 macrosetae in retroinferior series of basitarsus, 12–19 ventral macrosetae on telotarsus; pectine teeth: 17–21 (males), 15–18 (females).Published as part of Lowe, Graeme & Kovařík, František, 2016, Scorpions of the Horn of Africa (Arachnida, Scorpiones). Part V. Two new species of Neobuthus Hirst, 1911 (Buthidae), from Ethiopia and Eritrea, pp. 1-46 in Euscorpius 224 on page
D-0469: 121 North 100 West, Logan, Utah, Eba P. Lowe/Bertha C. Colby residence. Lot 1 Block 20 / Plat A. Built 1909
No full textD-0469: 121 North 100 West, Logan, Utah, Eba P. Lowe/Bertha C. Colby residence. Lot 1 Block 20 / Plat A. Built 190
Four Lowe complex artifacts recovered in controlled excavations in southern Belize.
No full text(A) Lowe point from TY. (B) Biface distal tip with unifacial beveling on alternate edges from TY. (C) Lowe point from MHCP. (D) Large but badly damaged Lowe point from MHCP. Scales in cm.</p
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