267 research outputs found
Turicibacter bilis Maki & Looft 2022, SP. NOV.
No full textDESCRIPTION OF TURICIBACTER BILIS SP. NOV. Turicibacter bilis (bi'lis. L. gen. n. bilis of bile). Strictly anaerobic, Gram-positive, catalase-negative, non-motile, spore-forming, pleomorphic micro-organisms. Supplementation of media with 0.1% (v/v) chicken bile induces spore germination. Growth is poor in broth alone, but improves significantly in biphasic media or broth with an agar plug. Cells primarily exist as long chains of irregular rods (0.8–8.2×0.5–2.0 μm) or as individual or clusters of coccoid cells (1.0–2.3 μm). The organism is capable of growth between 30–45 °C and at pH 6.5–8.5, with optimums of 42 °C and pH 7.5, respectively. Colonies are visible on BHIGL agar after 1–2 days and appear as small to mediumsized irregularly shaped, umbonate colonies with undulating margins, with the raised centre an opaque white that transitions to a translucent tan or grey at the margins. Colonies are 2.5–5.0 mm after 3 days at 42 °C. The primary fermentation end products are acetate, butyrate and lactate. The organism is positive for arginine and gelatin biochemical reactivity. The type strain is MMM721 T and was isolated from the surface of a chicken eggshell (= ATCC TSD-238 T = CCUG 74757 T). The genome is 2.7 Mb with a G+C content of 34.4mol%.Published as part of Maki, Joel J. & Looft, Torey, 2022, Turicibacter bilis sp. nov., a novel bacterium isolated from the chicken eggshell and swine ileum, pp. 1-9 in International Journal of Systematic and Evolutionary Microbiology (005153) (005153) 72 (1) on page 8, DOI: 10.1099/ijsem.0.005153, http://zenodo.org/record/604875
‐Analogs of the Antitumor Antibiotic CC‐1065 by Two Consecutive Transition Metal‐Initiated Transformations
No full textNovel seco-analogs of CC-1065 1 were synthesized from comercially available nitroaniline by reduction, bromination, bisulfonation and bisallylation followed by reaction with tert-butyllithium, zirconocene and iodine. The obtained quinoline 6 was then transformed into 17 and 18, which, upon treatment with Pd-0, led to 21 and 22, respectively. (C) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2003
Fig. 1 in Turicibacter bilis sp. nov., a novel bacterium isolated from the chicken eggshell and swine ileum
No full textFig. 1. (a) Transmission electron microscope image of MMM721T cells. Bar, 1 Μm. (b) Scanning electron microscope image of MMM721T cells. Bar, 2 Μm. Cells were cultured in BHIGL for 24 h at 42 °C prior to fixation and visualization.Published as part of Maki, Joel J. & Looft, Torey, 2022, Turicibacter bilis sp. nov., a novel bacterium isolated from the chicken eggshell and swine ileum, pp. 1-9 in International Journal of Systematic and Evolutionary Microbiology (005153) 72 (1) on page 3, DOI: 10.1099/ijsem.0.005153, http://zenodo.org/record/604875
Comparison of estimated breeding values, daughter yield deviations and de-regressed proofs within a whole genome scan for QTL
No full textThe definitive version is available at www.blackwell-synergy.comAn important issue in quantitative trait loci (QTL) detection is the use of phenotypic measurement as a dependent variable. Daughter yield deviations (DYDs) as the unit of choice are not available for all traits of interest. The use of de-regressed proofs (DRPFs) of estimated breeding values (EBVs) is an alternative to using daughter yield deviations. The objective of this study was to examine possible differences between DYDs and DRPFs within the use of QTL detection. The pedigree used was part of the granddaughter design of the German QTL effort. Consisting marker maps for livestock species were derived from all available data of 16 German Holstein paternal half-sib families with a total of 872 sires. The number of progeny ranged from 19 to 127. A whole genome scan was performed using weighted and unweighted multimarker regression with DYDs, DRPFs and EBVs as dependent variables for the traits milk, fat and protein yields. Results were compared with respect to the number of QTL detected. A similar number of QTL was detected with DRPFs and DYDs. Also, when dependent variables were weighted according to the variance of the trait, a higher number of QTL was detected at the desired level of significance as compared to using unweighted variables.H Thomsen, N Reinsch, N Xu, C Looft, S Grupe, C Kühn, G. A Brockmann, M Schwerin, B Leyhe-Horn, S Hiendleder, G Erhardt, I Medjugorac, I Russ, M Förster, B Brenig, F Reinhardt, R Reents, J Blümel, G Averdunk, E Kal
Quantitative trait loci mapping of functional traits in the German Holstein cattle population
No full textCopyright © 2003 American Dairy Science AssociationA whole-genome scan to detect quantitative trait loci (QTL) for functional traits was performed in the German Holstein cattle population. For this purpose, 263 genetic markers across all autosomes and the pseudoautosomal region of the sex chromosomes were genotyped in 16 granddaughter-design families with 872 sons. The traits investigated were deregressed breeding values for maternal and direct effects on dystocia (DYSm, DYSd) and stillbirth (STIm, STId) as well as maternal and paternal effects on nonreturn rates of 90 d (NR90m, NR90p). Furthermore, deregressed breeding values for functional herd life (FHL) and daughter yield deviation for somatic cell count (SCC) were investigated. Weighted multimarker regression analyses across families and permutation tests were applied for the detection of QTL and the calculation of statistical significance. A ten percent genomewise significant QTL was localized for DYSm on chromosome 8 and for SCC on chromosome 18. A further 24 putative QTL exceeding the 5% chromosomewise threshold were detected. On chromosomes 7, 8, 10, 18, and X/Yps, coincidence of QTL for several traits was observed. Our results suggest that loci with influence on udder health may also contribute to genetic variance of longevity. Prior to implementation of these QTL in marker assisted selection programs for functional traits, information about direct and correlated effects of these QTL as well as fine mapping of their chromosomal positions is required.Ch. Kühn, J. Bennewitz, N. Reinsch, N. Xu, H. Thomsen, C. Looft, G. A. Brockmann, M. Schwerin, C. Weimann, S. Hiendleder, G. Erhardt, I. Medjugorac, M. Förster, B. Brenig, F. Reinhardt, R. Reents, I. Russ, G. Averdunk, J. Blümel and E. Kal
Association of PPARGC1A and CAPNS1 gene polymorphisms and expression with meat quality traits in pigs
No full textThis study aimed to investigate the genes PPARGC1A (peroxisome proliferator-activated receptor gamma-coactivator 1A) and CAPNS1 (calpain small subunit 1) as candidate genes affecting meat quality traits in pigs. Four polymorphisms were identified in PPARCG1A and three in CAPNS1. The PPARGC1A polymorphism c.1288T>A was associated with pH and cooking loss in a F2 Duroc×Pietrain experimental cross (DuPi, n=313) and with pH values in Italian Large White (ILW, n=380) and Italian Landrace (ILA, n=158) populations (P<0.05). The CAPNS1 polymorphism c.429A>C was associated with pH and conductivity in DuPi and with meat color in ILA (P<0.05). PPARGC1A mRNA expression associated with drip loss (P<0.01) and the same tendency was found for CAPNS1 (P=0.06). The promoter methylation profiling suggested that methylation is not involved in CAPNS1 expression regulation. In conclusion, porcine PPARGC1A and CAPNS1 genes may affect meat quality traits, with breed-specific differences, and they could be used as markers for the improvement of meat quality in pigs
Genome-wide association analyses for boar taint components and testicular traits revealed regions having pleiotropic effects
No full textThe aim of this study was to perform a genome-wide association analyses (GWAS) for androstenone, skatole and indole in different Pietrain sire lines and compare the results with previous findings in purebred populations. Furthermore, the genetic relationship of androstenone and skatole were investigated with respect to pleiotropy. In order to characterize the performance of intact boars, crossbred progenies of 136 Pietrain boars mated to crossbred sows from three different breeding companies were tested on four test stations. A total of 598 boars were performance tested according to the rules of stationary performance testing in Germany. Beside common fattening and carcass composition traits, the concentrations of the boar taint components and testicular size parameters were recorded. All boars were genotyped with the PorcineSNP60 Illumina BeadChip. The GWAS were performed using the whole data set as well as in sub groups according to the line of origin. Besides an univariate GWAS approach, principal component (PC) techniques were applied to identify common expression pattern affecting the biosynthesis and the metabolism of androstenone
Isolation and assignment of the UDP-glucose pyrophosphorylase gene (UGP2) to porcine chromosome 3q21 -> q22 by FISH and by analysis of somatic cell and radiation hybrid panels
No full textA whole genome scan for differences in recombination rates among three Bos taurus breeds
No full textThe original publication can be found at www.springerlink.comTwenty paternal half-sib families of a granddaughter design were genotyped for 265 genetic markers, most of them microsatellites. These were 16 Holstein families, 3 Simmental families, and 1 Brown Swiss family. The number of sires per breed was 872, 170, and 32, respectively. Two-point recombination rates were estimated both jointly for all breeds and each single breed separately. Of 1168 marker intervals, 865 provided estimates for at least two breeds. Differences between breeds were tested by likelihood ratio tests. Four marker intervals, representing three genomic regions on BTA19, BTA24, and BTA27, show a significant impact of the breed at a false discovery rate of 0.23 and indicate a genetic component of observed heterogeneity of recombination. The variability of recombination rates between cattle breeds might not be a common feature of the whole genome, but rather might be restricted to certain chromosomal segments. Thus, attention should be paid to heterogeneities when pooling data of such regions from different breeds.Hauke Thomsen, Norbert Reinsch, Ningying Xu, Jörn Bennewitz, Christian Looft, Sven Grupe, Christa Kühn, Gudrun A. Brockmann, Manfred Schwerin, Birgit Leyhe-Horn, Stefan Hiendleder, Georg Erhardt, Ivica Medjugorac, Ingolp Russ, Martin Förster, Bertram Brenig, Fritz Reinhardt, Reinhard Reents, Jürgen Blümel, Gottfried Averdunk, Ernst Kal
The Iowa Homemaker vol.7, no.8
No full textMemorial Hall by Ruth E. Morrison, Antescript
Dawn’s Awakening by Irma D. Garner, Antescript
The Evolution of Women’s Clubs by Margaret Looft, page 1
Farm and Home Week by Sylvia Pedersen, page 2
How Much Shall I Buy? by Margaret L. Marnette, page 4
Marketing in Egypt by Frances Thomas, page 5
Girls’ 4-H Page by Corline Morrell, page 6
Iowa State Home Economics Association by Marcia E. Turner, page 8
Home Economics Research at Iowa State by Harriett C. Brigham, page 10
Who’s There and Where by Vera Caulum, page 12
Better Homes Week by Kathryn Davis, page 13
Foreign Students Will Occupy “The Gables” by Ruth E. Morrison, page 14
Home Economics Vod-Vil by Gladys Parker, page 15</p
- …
