6,714 research outputs found
Huh-hah-hei ja huh-hah-hei (4/4 C)
No full textLaulun sanat: Huh-hah-hei ja huh-hah-hei, Ja ei oo meillä huolta, ei! Näin sitä mennään pyörähdellään, Pyörähdellään, pampampaa
Comparison of replication in HuH-7T1 and Huh-7.5.1.
No full text<p>(A) Five micrograms of JFH-1 subgenomic replicon RNA was electroporated into HuH-7T1 and Huh-7.5.1. The cells were harvested at indicated time points. The luciferase activity in the cell lysates was normalized to the data at 4 h after transfection; values are expressed as fold increases. (B and C) Comparison of transfection and translation efficiencies. Five micrograms of JFH-1/GND-Luc RNA was transfected into HuH-7T1 and Huh-7.5.1. The cells were harvested at 4 h after transfection, and the amount of transfected RNA in cells (B) and luciferase activity in the cell lysates (C) were measured.</p
Replication of Huh-7 – passaged cloned DENV-2.
No full text<p>(a) Huh-7 – passaged sylvatic P8-1407 DENV-2 on Huh-7 cells. (b) Huh-7 – passaged endemic IQT-1950 DENV-2 on Huh-7 cells. (c) Huh-7 – passaged sylvatic P8-1407 DENV-2 on the bypassed cell line C6/36. (d) Huh-7 – passaged endemic IQT-1950 DENV-2 on the bypassed cell line C6/36. (e) Huh-7 – passaged sylvatic P8-1407 DENV-2 on a control cell line (Vero). (f) Huh-7 – passaged endemic IQT-1950 DENV-2 on a control cell line (Vero). Timepoint T = 0 represents residual virus after washing.</p
Replication of Huh-7 – passaged uncloned DENV-2.
No full text<p>(a) Huh-7 – passaged sylvatic P8-1407 DENV-2 on Huh-7 cells. (b) Huh-7 – passaged endemic IQT-1950 DENV-2 on Huh-7 cells. (c) Huh-7 – passaged sylvatic P8-1407 DENV-2 on the bypassed cell line C6/36. (d) Huh-7 – passaged endemic IQT-1950 DENV-2 on the bypassed cell line C6/36. (e) Huh-7 – passaged sylvatic P8-1407 DENV-2 on a control cell line (Vero). (f) Huh-7 – passaged endemic IQT-1950 DENV-2 on a control cell line (Vero). Timepoint T = 0 represents residual virus after washing.</p
Prohibitin is overexpressed in Huh-7-HCV and Huh-7.5-HCV cells harboring in vitro transcribed full-length hepatitis C virus RNA
No full textAbstract Background Currently, up-regulated proteins and apoptosis in hepatitis C is a hot topic in exploring the pathogenic mechanism of Heptitis C Virus(HCV). Some recent studies shows that prohibitin is overexpressed in cells expressing HCV core proteins, and up-regulated prohibitin is also found in human hepatoma cell line HCC-M, lung cancer, prostate cancer, and other cancers. Prohibitin is an important member of the membrane protein superfamily, and it plays a role of molecular chaperones in mitochondrial protein stability. Meanwhile, it has a permissive action on tumor growth or acts as an oncosuppressor. Based on our previously established the in vitro HCV cell-culture system (HCVcc), here we aimed to investigate the different expression profiles of prohibitin in Huh-7-HCV and Huh-7.5-HCV cells Methods The total cellular RNA of Huh-7, Huh-7.5, Huh-7-HCV and Huh-7.5-HCV cells were extracted, and then the first-strand cDNA was reversely transcribed. The expression of prohibitin at the mRNA level was assessed by real-time PCR with GAPDH as the control. Furthermore, the expression of prohibitin at the protein level was evaluated by western blot with GAPDH as an internal control. Results Our results of real-time PCR showed that the mRNA expression level of prohibitin in Huh-7-HCV cells was 2.09 times higher than that in Huh-7 cells, while, the mRNA level of prohibitin in Huh-7.5-HCV cells was 2.25 times higher than that in Huh-7.5 cells. The results of western blot showed that the protein expression level of prohibitin in Huh-7-HCV cells was 2.38 times higher than that in Huh-7 cells, while the protein expression of prohibitin in Huh-7.5-HCV cells was 2.29 times higher than that in Huh-7.5 cells. Conclusions The expression of prohibitin was relatively high in Huh-7-HCV and Huh-7.5-HCV cells harboring in vitro transcribed full-length HCV RNA.</p
EFFECTS OF THE KARLOVITZ NUMBER ON THE EVOLUTION OF THE FLAME SURFACE DENSITY IN TURBULENT PREMIXED FLAMES
No full textDirect numerical simulation (DNS) is conducted to investigate the effects of the Karlovitz number (Ka) on displacement speed and consequent evolution of flame surface density (FSD). Parametric study is performed for the Ka between 2 and 10 in the thin reaction zone regime with independent variation of laminar flame speed and turbulent intensity. Previous study showed the effect of the turbulent intensity without noticeable influence of the Ka lower than 2.4 [I. Han, K.Y. Huh, Combust. Flame 152 (2008) 194-205]. A higher Ka involves a lower displacement speed on the positive curvature side primarily due to the influence on the normal diffusion component. It leads to a negative curvature term to act as a sink for FSD throughout a flame brush. The maximum FSD increases with increasing turbulent intensity, while a higher Ka leads to an asymmetric profile of FSD due to suppressed production at the leading edge. A higher Ka decreases total flame area and turbulent burning velocity as well, while a limiting behavior is shown for low Da cases. (C) 2009 The Combustion Institute. Published by Elsevier Inc. All rights reserved.X1131sciescopu
210Pb, 137Cs and 239,240Pu in East China Sea Sediments: Sources, Pathways and Budgets of Sediments and Radionuclides
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