832 research outputs found
Among the Deep Sea Fishers, volume 60, issue 1 (April 1962)
No full textThe decline and fall of pulmonary tuberculosis / Gordon W. Thomas -- Sometimes by dogteam / Mary K. J. Stephens -- Puppets and mummers / Ella E. Hewitt -- Ice fishing is a cold business / Marjorie G. Nichols -- New supply vessel -- Off and on the coast -- Our staff -- The loss of the Stella Maris / George C. Whiteley -- The Grenfell Associations -- Alumni -- Clothing contributions -- Spring events.Includes map: [Newfoundland and Labrador] (inside front cover).Among the Deep Sea Fishers: the Official Organ of the International Grenfell Association. This journal was published quarterly from 1903 to 1981 with the twofold purpose of providing "a record of Mission activities [and] also a strong and convincing appeal to every supporter and friend of Dr. Grenfell's work." The articles describe mission life, services and experiences. The Mission began under the auspices of the Royal National Mission to Deep Sea Fishermen but later a separate mission, the International Grenfell Association, was formed by Dr. Wilfred Grenfell
The spruce budworm and larch sawfly lecture delivered by Dr. C. Gordon Hewitt, F.E.S., Dominion entomologist at the Canadian Forestry Convention, city of Qu\ue9bec, 19th January, 1911
No full textTranscriptional profiling of the postnatal brain of the Ts1Cje mouse model of Down syndrome
No full textAbstract not availableKai-Leng Tan, King-Hwa Ling, Chelsee A. Hewitt, Pike-See Cheah, Ken Simpson, Lavinia Gordon, Melanie A. Pritchard, Gordon K. Smyth, Tim Thomas, Hamish S. Scot
The spruce budworm and larch sawfly lecture delivered by Dr. C. Gordon Hewitt, F.E.S., Dominion entomologist at the Canadian Forestry Convention, city of Qu\ue9bec, 19th January, 1911
No full textSpatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the Nrgn and Camk2n1 gene loci during mouse cerebral corticogenesis
No full textFirst published online: August 6, 2010Nrgn and Camk2n1 are highly expressed in the brain and play an important role in synaptic long-term potentiation via regulation of Ca2+/calmodulin-dependent protein kinase II. We have shown that the gene loci for these 2 proteins are actively transcribed in the adult cerebral cortex and feature multiple overlapping transcripts in both the sense and antisense orientations with alternative polyadenylation. These transcripts were upregulated in the adult compared with embryonic and P1.5 mouse cerebral cortices, and transcripts with different 3′ untranslated region lengths showed differing expression profiles. In situ hybridization (ISH) analysis revealed spatiotemporal regulation of the Nrgn and Camk2n1 sense and natural antisense transcripts (NATs) throughout cerebral corticogenesis. In addition, we also demonstrated that the expression of these transcripts was organ-specific. Both Nrgn and Camk2n1 sense and NATs were also upregulated in differentiating P19 teratocarcinoma cells. RNA fluorescent ISH analysis confirmed the capability of these NATs to form double-stranded RNA aggregates with the sense transcripts in the cytoplasm of cells obtained from the brain. We propose that the differential regulation of multiple sense and novel overlapping NATs at the Nrgn and Camk2n1 loci will increase the diversity of posttranscriptional regulation, resulting in cell- and time-specific regulation of their gene products during cerebral corticogenesis and function.King-Hwa Ling, Chelsee A. Hewitt, Tim Beissbarth, Lavinia Hyde, Pike-See Cheah, Gordon K. Smyth, Seong-Seng Tan, Christopher N. Hahn, Tim Thomas, Paul Q. Thomas and Hamish S. Scot
Molecular networks involved in mouse cerebral corticogenesis and spatio-temporal regulation of Sox4 and Sox11 novel antisense transcripts revealed by transcriptome profiling
No full textThe electronic version of this article is the complete one and can be found online at: http://genomebiology.com/2009/10/10/R104Background: Development of the cerebral cortex requires highly specific spatio-temporal regulation of gene expression. It is proposed that transcriptome profiling of the cerebral cortex at various developmental time points or regions will reveal candidate genes and associated molecular pathways involved in cerebral corticogenesis. Results: Serial analysis of gene expression (SAGE) libraries were constructed from C57BL/6 mouse cerebral cortices of age embryonic day (E) 15.5, E17.5, postnatal day (P) 1.5 and 4 to 6 months. Hierarchical clustering analysis of 561 differentially expressed transcripts showed regionalized, stage-specific and co-regulated expression profiles. SAGE expression profiles of 70 differentially expressed transcripts were validated using quantitative RT-PCR assays. Ingenuity pathway analyses of validated differentially expressed transcripts demonstrated that these transcripts possess distinctive functional properties related to various stages of cerebral corticogenesis and human neurological disorders. Genomic clustering analysis of the differentially expressed transcripts identified two highly transcribed genomic loci, Sox4 and Sox11, during embryonic cerebral corticogenesis. These loci feature unusual overlapping sense and antisense transcripts with alternative polyadenylation sites and differential expression. The Sox4 and Sox11 antisense transcripts were highly expressed in the brain compared to other mouse organs and are differentially expressed in both the proliferating and differentiating neural stem/progenitor cells and P19 (embryonal carcinoma) cells. Conclusions: We report validated gene expression profiles that have implications for understanding the associations between differentially expressed transcripts, novel targets and related disorders pertaining to cerebral corticogenesis. The study reports, for the first time, spatio-temporally regulated Sox4 and Sox11 antisense transcripts in the brain, neural stem/progenitor cells and P19 cells, suggesting they have an important role in cerebral corticogenesis and neuronal/glial cell differentiation.King-Hwa Ling, Chelsee A Hewitt, Tim Beissbarth, Lavinia Hyde, Kakoli Banerjee, Pike-See Cheah, Ping Z Cannon, Christopher N Hahn, Paul Q Thomas, Gordon K Smyth, Seong-Seng Tan, Tim Thomas and Hamish S Scot
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