124 research outputs found
Angiographic computed tomography is comparable to multislice computed tomography in lumbar myelographic imaging
No full textWe evaluated the feasibility of angiographic computed tomography (ACT) for diagnostic imaging in a patient with degenerative lumbar spine disease. ACT provides a volume data set out of a rotational acquisition with a c-arm-mounted flat-panel detector. Using this technique, radiographic myelography and myelographic computed tomography can be performed in a single session at the same imaging system. The quality of the reconstructed ACT slice images is comparable to those acquired by postmyelographic computed tomography
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
No full textNeurotransmitter uptake into synaptic vesicles is mediated by vesicular neurotransmitter transporters. Although these transporters belong to different families, they all are thought to share a common overall topology with an even number of transmembrane domains. Using epitope-specific antibodies and mass spectrometry we show that the vesicular GABA transporter (VGAT) possesses an uneven number of transmembrane domains, with the N terminus facing the cytoplasm and the C terminus residing in the synaptic vesicle lumen. Antibodies recognizing the C terminus of VGAT (anti-VGAT-C) selectively label GABAergic nerve terminals of live cultured hippocampal and striatal neurons as confirmed by immunocytochemistry and patch-clamp electrophysiology. Injection of fluorochromated anti-VGAT-C into the hippocampus of mice results in specific labeling of GABAergic synapses in vivo. Overall, our data open the possibility of studying novel GABA release sites, characterizing inhibitory vesicle trafficking, and establishing their contribution to inhibitory neurotransmission at identified GABAergic synapses
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No full textSmall GTPases of the Rab family not only regulate target recognition in membrane traffic but also control other cellular functions such as cytoskeletal transport and autophagy. Here we show that Rab26 is specifically associated with clusters of synaptic vesicles in neurites. Overexpression of active but not of GDP-preferring Rab26 enhances vesicle clustering, which is particularly conspicuous for the EGFP-tagged variant, resulting in a massive accumulation of synaptic vesicles in neuronal somata without altering the distribution of other organelles. Both endogenous and induced clusters co-localize with autophagy-related proteins such as Atg16L1, LC3B and Rab33B but not with other organelles. Furthermore, Atg16L1 appears to be a direct effector of Rab26 and binds Rab26 in its GTP-bound form, albeit only with low affinity. We propose that Rab26 selectively directs synaptic and secretory vesicles into preautophagosomal structures, suggesting the presence of a novel pathway for degradation of synaptic vesicles
Das unvergeßliche Andencken, Welches Der weyland Wohl-Edle und Wohlgelahrte Herr Johann Ernst Henffling, I. V. C. Durch ein ansehnliches Legatvm An das Fürstl. Sachßen-Meiningische Inlvstre Lyceum Sich selbsten aufgerichtet, Suchte Bey Solenner Einführung Der sechs erstern Henfflingischen Convictoristen in Segen zu erhalten Und zugleich zu einer öffentlichen Red-Ubung einzuladen Alle Beförderer ... M. Christoph Albrecht Erck, Gedachten Lycei Inspector
No full textVorlageform des Erscheinungsvermerks: Mejnjngen, Gedruckt bey Jonas Chr. Hasserts, F. S. Hof. Buchdr. seel. hinterlassenen Wittbe. - Erscheinungsjahr nach Datierung am Textend
Das unvergeßliche Andencken, Welches Der weyland Wohl-Edle und Wohlgelahrte Herr Johann Ernst Henffling, I. V. C. Durch ein ansehnliches Legatvm An das Fürstl. Sachßen-Meiningische Inlvstre Lyceum Sich selbsten aufgerichtet, Suchte Bey Solenner Einführung Der sechs erstern Henfflingischen Convictoristen in Segen zu erhalten Und zugleich zu einer öffentlichen Red-Ubung einzuladen Alle Beförderer ... M. Christoph Albrecht Erck, Gedachten Lycei Inspector
No full textVorlageform des Erscheinungsvermerks: Mejnjngen, Gedruckt bey Jonas Chr. Hasserts, F. S. Hof. Buchdr. seel. hinterlassenen Wittbe. - Erscheinungsjahr nach Datierung am Textend
Constitutive expression of murine c-FLIPR causes autoimmunity in aged mice.
No full textDeath receptor-mediated apoptosis is a key mechanism for the control of immune responses and dysregulation of this pathway may lead to autoimmunity. Cellular FLICE-inhibitory proteins (c-FLIPs) are known as inhibitors of death receptor-mediated apoptosis. The only short murine c-FLIP splice variant is c-FLIPRaji (c-FLIPR). To investigate the functional role of c-FLIPR in the immune system, we used the vavFLIPR mouse model constitutively expressing murine c-FLIPR in all hematopoietic compartments. Lymphocytes from these mice are protected against CD95-mediated apoptosis and activation-induced cell death. Young vavFLIPR mice display normal lymphocyte compartments, but the lymphocyte populations alter with age. We identified reduced levels of T cells and slightly higher levels of B cells in 1-year-old vavFLIPR mice compared with wild-type (WT) littermates. Moreover, both B and T cells from aged vavFLIPR animals show activated phenotypes. Sera from 1-year-old WT and transgenic animals were analysed for anti-nuclear antibodies. Notably, elevated titres of these autoantibodies were detected in vavFLIPR sera. Furthermore, tissue damage in kidneys and lungs from aged vavFLIPR animals was observed, indicating that vavFLIPR mice develop a systemic lupus erythematosus-like phenotype with age. Taken together, these data suggest that c-FLIPR is an important modulator of apoptosis and enforced expression leads to autoimmunity
Radiation-Induced Segregation and Precipitation in Ion-Irradiated Molybdenum-Rhenium Alloys
No full textRadiation-induced solute segregation and precipitation in molybdenum-rhenium solid-solution alloys were studied during elevated-temperature helium- and neon-ion irradiation. Radiation-induced segregation of Re atoms in the same direction as the defect fluxes, that is, toward the external surface, was measured during irradiation by in situ Rutherford backscattering spectrometry (RBS). The results of the segregation measurements were in agreement with theoretical models based on point-defect driven transport of solute atoms toward point-defect sinks.After irradiation, near-surface microstructural changes were studied using transmission-electron microscopy (TEM). Radiation-induced chi-phase precipitates were identified. The precipitates adopted coincident and (111) twin orientations with respect to the matrix. The twinning morphology was explained in terms of a coincident-site-lattice model.The results of the RBS and TEM studies constitute strong evidence that precipitation of the chi phase occurs in Mo-Re solid-solution alloys when radiation-induced solute segregation causes the equilibrium solubility limit to be exceeded near point-defect sinks.Made available in DSpace on 2014-12-16T20:52:19Z (GMT). No. of bitstreams: 1
8908673.pdf: 5821313 bytes, checksum: f5c85fdde8a0035545a29f59c1613076 (MD5)
Previous issue date: 1988Embargo set by: Seth Robbins for item 72021
Lift date: Forever
Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDsRestricted to the U of I community idenfinitely during batch ingest of legacy ETDsU of I Only213 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1988
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