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    Implementation of SELEX technique on Lab-on-Chip systems

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    The thesis presents the design and the experimental development of a compact and high sensitive Lab-on-Chip (LoC) system suitable for the implementation of SELEX technique. SELEX (Systematic Evolution of Ligands by EXponential enrichment) is a combinatorial technique used in molecular biology to produce copies of the same nucleotide and to select a strand of DNA (aptamer) specific for a target molecule. The proposed Lab-On-Chip system includes the following functional units: an amplification module based on the PCR technique; a separation module able to obtain a single strand DNA from a double strand DNA and a selection module for the specific selection of the aptamer. These functionalities are implemented combining microfluidic components (micro-channels and micro-valves), electronic devices (amorphous silicon photosensors, thin film heaters, temperature sensors) and bioanalytical procedure

    Disposable screen printed potentiometric sensors for determination of free radicals

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    in C. Di Natale, A. D'Amico (Ed.), Sensors and Microsystems, World Sci., Singapor

    ON-LINE FULLY AUTOMATED MEASUREMENT OF CR(VI) CONCENTRATION IN WATER BY A FIBER OPTIC PROBE AND A LOW COST SPECTROPHOTOMETER

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    A measurement system conjugating the flow-injection technique in an automatic system and a low cost spectrometer as the photo-detecting device has been set in order to evaluate the concentration of Chromium (VI) in water. Light absorption spectra of the complex Chromium (VI) – Diphenylcarbazide, carried into the measuring cell, were analysed to detect the effect of interfering quantities and substances by also operating with suitable data processing techniques, like neural network. The aim of this work is to check the feasibility of this approach, for on-line and distributed measurements. Aspects concerning instrumentation system and operating steps are analysed in order to evaluate uncertainty causes and their effects on the whole uncertainty of the methodology, taking into account requirements of method reproducibility and standardization
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