51 research outputs found
Estimation of anisotropic permeability in trabecular bone based on microCT imaging and pore-scale fluid dynamics simulations
No full textAbstract not availableC. Daish, R. Blanchard, K. Gulati, D. Losic, D. Findlay, D.J.E. Harvie, P. Pivonk
Platypus chain reaction: directional and ordered meiotic pairing of the multiple sex chromosome chain in Ornithorhynchus anatinus
No full text© CSIRO 2009Monotremes are phylogenetically and phenotypically unique animals with an unusually complex sex chromosome system that is composed of ten chromosomes in platypus and nine in echidna. These chromosomes are alternately linked (X1Y1, X2Y2, ...) at meiosis via pseudoautosomal regions and segregate to form spermatozoa containing either X or Y chromosomes. The physical and epigenetic mechanisms involved in pairing and assembly of the complex sex chromosome chain in early meiotic prophase I are completely unknown. We have analysed the pairing dynamics of specific sex chromosome pseudoautosomal regions in platypus spermatocytes during prophase of meiosis I. Our data show a highly coordinated pairing process that begins at the terminal Y5 chromosome and completes with the union of sex chromosomes X1Y1. The consistency of this ordered assembly of the chain is remarkable and raises questions about the mechanisms and factors that regulate the differential pairing of sex chromosomes and how this relates to potential meiotic silencing mechanisms and alternate segregation.Tasman Daish, Aaron Casey and Frank Grützne
Deciphering the secret role of Sox4 gene locus during mouse cerebral corticogenesis
No full textAbstract no. S03-3. DOI: http://www.dx.doi.org/10.1111/jnc.12775K. H. Ling, H. C. Lee, P. J. Brautigan, S. Moore, R. Fraser, P. S. Cheah, J. M. Raison, M. Babic, Y. K. Lee, T. Daish, D. M. Mattiske, J. R. Mann, D. L. Adelson, P. Q. Thomas, C. N. Hahn, H. S. Scot
Ecdysone-induced expression of the caspase DRONC during hormone-dependent programmed cell death in Drosophila is regulated by Broad-Complex
No full text© Rockefeller University PressThe steroid hormone ecdysone regulates both cell differentiation and cell death during insect metamorphosis, by hierarchical transcriptional regulation of a number of genes, including the Broad-Complex (BR-C), the zinc finger family of transcription factors. These genes in turn regulate the transcription of a number of downstream genes. DRONC, a key apical caspase in Drosophila, is the only known caspase that is transcriptionally regulated by ecdysone during development. We demonstrate that dronc gene expression is ablated or reduced in BR-C mutant flies. Using RNA interference in an ecdysone-responsive Drosophila cell line, we show that DRONC is essential for ecdysone-mediated cell death, and that dronc upregulation in these cells is controlled by BR-C. Finally, we show that the dronc promoter has BR-C interaction sites, and that it can be transactivated by a specific isoform of BR-C. These results indicate that BR-C plays a key role in ecdysone-mediated caspase regulation.Dimitrios Cakouros, Tasman Daish, Damali Martin, Eric H. Baehrecke and Sharad Kuma
Investigation of heat transfer enhancement in dimpled pipe flows
No full textIt is well established that dimpling of tubes can help enhance the heat convection coefficient. The effects of the dimples shape, depth and distribution on heat transfer and pressure drop for laminar and turbulent flows are of interest to this study. The aim of this paper is to quantify the effects of dimpling on the performance of 11mm ID tubes used in a submarine shell and tube heat exchanger. Both experiments and calculations were conducted, using water as the working fluid, over a wide range of Reynolds numbers ranging from 1500 to 24000, based on bulk velocity and tube diameter. Four tubes with different dimpling arrangements were tested. The CFD results show that a tube with 2mm deep single dimples arrangement, operating in the laminar flow regime, gives the maximum improvements in heat transfer of 10.23% over the smooth tube. The largest pressure drop increase recorded was 102.8% under identical flow conditions for the same dimpled tube. Temperature contour plots were used to demonstrate the mixing effect of the dimples under laminar flow conditions inside the tube.A. W. Albanesi, K. D. Daish, B. Dally and R. C. Chi
Derivation of an endogenous small RNA from double-stranded Sox4 sense and natural antisense transcripts in the mouse brain
No full textAbstract not availableKing-Hwa Ling, Peter J. Brautigan, Sarah Moore, Rachel Fraser, Pike-See Cheah, Joy M. Raison, Milena Babic. Young Kyung Lee, Tasman Daish, Deidre M. Mattiske, Jeffrey R. Mann, David L. Adelson, Paul Q. Thomas, Christopher N. Hahn, Hamish S. Scot
The bone people and the architecture of 'home'
No full textThis thesis addresses the novel The Bone People to explore the question of what is the architecture of 'home'. The book is used because the characters in The Bone People are on a constant search for a home, and in the process the narrative moves between a number of different domestic architectures. The relationships the characters have with their houses, and the symbolism of the houses, reveals the importance architecture can have in our concepts of home, and this architecture is always inclusive of its inhabitants. Part One introduces the author, the main character and their houses, and then presents architectural theories on house and home that are relevant to this work. In the second part the figures of Kerewin's Tower and Joe's State house are given as examples of houses that reflect their owners and show signs of uncanny (unhomely) qualities. The baches are then put forward as New Zealand icons and places of change and discovery. Part Three explores the whare as representing a traditional way of life that is dying, and the hut as a universal site for rejuvenation in nature. The children's 'homes' are places where Simon is definitely not at home, and the Maori hall becomes the 'home' marae for the local community. The final example of the shell house is presented as an expression of hope for the characters, and possibility for New Zealand architecture. I conclude that the architecture in The Bone People is fundamental to the novel as a whole because it structures the plot, and is used to understand the characters and their experiences of house and home. In The Bone People the architecture of 'home' is a place where people who care for one another live, and to have a home in this world is vitally significant as a basic human need
The Drosophila melanogaster Apaf-1 homologue ARK is required for most, but not all, programmed cell death
No full text© The Rockefeller University PressThe Apaf-1 protein is essential for cytochrome c–mediated caspase-9 activation in the intrinsic mammalian pathway of apoptosis. Although Apaf-1 is the only known mammalian homologue of the Caenorhabditis elegans CED-4 protein, the deficiency of apaf-1 in cells or in mice results in a limited cell survival phenotype, suggesting that alternative mechanisms of caspase activation and apoptosis exist in mammals. In Drosophila melanogaster, the only Apaf-1/CED-4 homologue, ARK, is required for the activation of the caspase-9/CED-3–like caspase DRONC. Using specific mutants that are deficient for ark function, we demonstrate that ARK is essential for most programmed cell death (PCD) during D. melanogaster development, as well as for radiation-induced apoptosis. ark mutant embryos have extra cells, and tissues such as brain lobes and wing discs are enlarged. These tissues from ark mutant larvae lack detectable PCD. During metamorphosis, larval salivary gland removal was severely delayed in ark mutants. However, PCD occurred normally in the larval midgut, suggesting that ARK-independent cell death pathways also exist in D. melanogaster.Kathryn Mills, Tasman Daish, Kieran F. Harvey, Cathie M. Pfleger, Iswar K. Hariharan, and Sharad Kuma
Uncomfortable marriage: on the nature of the theory/practice relationship
No full textPart one
After a brief definition of both theory and practice part one goes on to examine the nature of their relationship. This examination reveals the underlying issues which gave motivation for the subsequent research. The main issues of concern to the author are:
- The problematic nature of the relationship between theory and practice.
- The importance of their interaction to achieve a responsible, legitimate and progressive architecture.
- Theory and practice are produced and operate within various contexts. If theory is to hold any relevance it must engage with the socio-political issues provided by these contexts.
Part two
Having established a theoretical framework from which to work in part one, part two draws on a specific architect and project (Bernard Tschumi's Parc de la Villette). Tschumi and La Villette are merely used as a vehicle for exploration. I felt that in order to test and explore the relationship of theory to practice it was essential to locate my argument around a real completed project. It is hoped that the interrogation of Tschumi's ideas and their concrete realisation in Parc de la Villette will provide some insight into the success/failure/indifference of Tschumi's attempt to bridge the theory/practice rift.
Part two begins then with a brief outline of the theoretical motivations behind Parc de la Villette. A look at the social and political context surrounding, the park follows, questioning whether Tschumi's theoretical intentions recognise and engage with these issues successfully.
The middle section of part two looks at Parc de la Villette as a theoretical work. It asks the question: can a built work satisfy the expectations of a (deconstructive) theory of architecture?
Finally part two proposes that although the interaction of theory and practice is crucial to achieve an architecture of substance, their very interaction seems to strip each of its potential. For example, the political potential of deconstructive theory seems to be negated by its practical application. The acknowledgement of the social, political and economic forces surrounding each architectural project is essential for the creation of a responsible and significant architecture
Shopping centres: a design analysis
No full textThis report surveys the "collective wisdom" of the design of shopping centres. Whilst undertaking this survey the history of shopping centres was briefly touched on. The survey consisted of reading, interviews with "experts" in the field of shopping centres and personal observation. The result of the survey showed that experience has developed a series of "rules" which are applied to shopping centre design.
This "collective wisdom" was then tested by applying it to a sample of three Wellington shopping centres by means of an architectural analysis devised by Roger H. Clark and Michael Pause in their book "Precedents in Architecture". (see appendix one for an excerpt from the book).
The results of this analysis, after being related to the "collective wisdom" showed that the main principles of the "collective wisdom" were applied and that there are several ways in which the "collective wisdom" can be applied.
The report conclude by briefly pointing towards the future of shopping centre design. This future reflects personal opinion of the author formed as a result of undertaking this study
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