902 research outputs found

    Seine. Le cellier de l'hôtel de Preuilly, rue Geoffroy L'Asnier à Paris

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    Lambert C. Seine. Le cellier de l'hôtel de Preuilly, rue Geoffroy L'Asnier à Paris. In: Bulletin Monumental, tome 144, n°4, année 1986. pp. 343-344

    Interaction of group-specific component (vitamin D-binding protein) with immobilized Cibacron blue F3-GA

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    Group-specific component (vitamin D-binding protein) was purified to homogeneity from human plasma by a three-step procedure involving pseudo-ligand affinity chromatography on immobilized Cibacron blue F3-GA followed by gel filtration and ion-exchange chromatography. Upon pseudo-ligand chromatography, Gc globulin was separated into two peaks. The first, which represented approx. 4% of the total Gc globulin, was eluted together with other alpha-globulins of similar Mr and/or pI, and the second (96% of Gc globulin) was clearly retarded. Collection of the latter provided a fraction 10-fold enriched in Gc globulin, with yields higher than 90%. Incubation of plasma with trace amounts of radioactively-labeled 25-OH vitamin D3 showed that the radioactivity coeluted with the first peak. In addition, after saturation with 25-OH vitamin D3, all the Gc globulin was eluted in the first peak. This indicates that the two peaks correspond to the holo and the apo forms of the protein, respectively, and suggests that either the interaction of the apo form with the Cibacron blue dye involves the binding site for vitamin D metabolites, or that the holo-protein undergoes a conformational change as a consequence of formation of the complex

    Contribution genetics to the understanding of coeliac disease

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    In Cerfbensussan N, Brousse N, Caillatzucman S, Cellier C, Schmitz J, Eitors, John Libbey Proccedings of the Xth International Symposium on Coeliac Diseas

    Molecular evolutionary analysis of the Nramp family

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    A review. The natural resistance-assocd. macrophage protein (Nramp) belongs to a family of ion permeases highly conserved in eukaryotes that originated in prokaryotes. Three phylogenetic groups of homologs were identified in Bacteria based on functional genomic approaches. Proteins of group A were characterized as H+-dependent Manganese Transporter (MntH A) representing functional homologs of eukaryotic Nramp. Heterologous expression in E. coli of MntH B and C proteins modulated bacterial sensitivity to cobalt and cadmium, suggesting conserved function among MntH groups. Genome sequencing of model eukaryotes revealed two groups of eukaryotic Nramp. Conservation in invertebrates of a 'prototype' suggests early duplication of an ancestral Nramp gene. Family trees inferred from phylogenetic analyses using various approaches indicate successive divergence of MntH B, MntH A, eukaryotic Nramp and MntH C. MntH C shows high level sequence identity with 'prototype' Nramp, heterogeneous variation of a.a. replacement rate among sites, and predicted divergence from 'archetype' Nramp. The data suggest a relatively recent origin for MntH C, derived from an eukaryote 'prototype' Nramp gene. This proposition is discussed in the context of bacterial infection.</br

    Mauves-sur-Loire – 938 rue du Cellier (lot 2)

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    Le terrain situé au 938 rue du Cellier (lot 2) à Mauves-sur-Loire (parcelle AE 162) a livré un fossé, probablement d’enclos, dont la fondation peut être attribuée au milieu du iie s. av. J.-C. (LT C2/D1), notamment grâce à deux amphores de type gréco-italique et Dressel 1A qui y ont été découvertes. Le comblement sommital de la structure, qui a fourni du mobilier antique (iie s. apr. J.-C.), suggère que la structure a été recreusée ou définitivement nivelée à cette époque. Fig. 1 – Plan génér..

    Celiac disease-related conditions: Who to test?

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    : Celiac disease (CeD) is a chronic immune-mediated condition triggered by gluten consumption in genetically predisposed individuals. Approximately 1% of the general population is affected by the disorder. Disease presentation is heterogeneous, and despite growing awareness among physicians and the public, it continues to be underestimated. The most effective strategy for identifying undiagnosed CeD is proactive case-finding through serologic testing in high-risk groups. We reviewed the most recent evidence on the association between CeD and over 20 conditions. In light of this review, CeD screening is recommended in individuals with (I) autoimmune disease and accompanying symptoms suggestive of CeD, (II) diseases that may mimic CeD (e.g., irritable bowel syndrome, inflammatory bowel disease, and microscopic colitis), and among patients with (III) conditions with a high CeD prevalence: first-degree relatives, idiopathic pancreatitis, unexplained liver enzyme abnormalities, autoimmune hepatitis, primary biliary cholangitis, hyposplenism or functional asplenia with severe bacterial infection, type 1 diabetes mellitus, Hashimoto's thyroiditis and Grave's disease, Sjögren's syndrome, dermatitis herpetiformis, recurrent aphthous syndrome and enamel defects, unexplained ataxia, peripheral neuropathy, delayed menarche or premature menopause, Down syndrome, Turner syndrome, Williams syndrome, chronic fatigue syndrome, IgA nephropathy, and IgA deficiency. CeD serology should be the initial step in the screening process. However, for patients with any of the aforementioned disorders who are undergoing upper endoscopy, biopsies should be performed to rule out CeD

    Renée Cellier, « Les exploitations viticoles d'une commune du Bas- Vivarais : étude socio-économique ». Mémoire pour le diplôme de FEPHE

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    Royer C. Renée Cellier, « Les exploitations viticoles d'une commune du Bas- Vivarais : étude socio-économique ». Mémoire pour le diplôme de FEPHE. In: Études rurales, n°47, 1972. pp. 197-198
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