1,458 research outputs found
eIF-2 initiation factor activity in postribosomal supernatant of hypertrophying rat diaphragm
No full textiIF-2 initiation factor activity in postribosomal supernatant of hypertrophying rat diaphragm.
Carraro U, Catani C.
FEBS Lett. 1980 Feb 11;110(2):173-6. No abstract available.
PMID:
7371823
[PubMed - indexed for MEDLINE
Myosin light and heavy chains in rat gastrocnemius and diaphragm muscles after chronic denervation or reinnervation
No full textMyosin light and heavy chains in rat gastrocnemius and diaphragm muscles after chronic denervation or reinnervation.
Carraro U, Catani C, Dalla Libera L.
Exp Neurol. 1981 May;72(2):401-12. No abstract available.
PMID:
7238699
[PubMed - indexed for MEDLINE
Selective maintenance of neurotrophically regulated proteins in denervated rat diaphragm
No full textExp Neurol. 1979 Mar;63(3):468-75.
Selective maintenance of neurotrophically regulated proteins in denervated rat diaphragm.
Carraro U, Catani C, Biral D.
PMID:
155010
[PubMed - indexed for MEDLINE
Differential distribution of tropomyosin subunits in fast and slow rat muscles and its changes in long-term denervated hemidiaphragm
No full textDifferential distribution of tropomyosin subunits in fast and slow rat muscles and its change in long-term denervated hemidiaphragm.
Carraro U, Catani C, Dalla Libera L, Vascon M, Zanella G.
FEBS Lett. 1981 Jun 15;128(2):233-6. No abstract available.
PMID:
7262316
[PubMed - indexed for MEDLINE
SVCT1 and SVCT2: Key proteins for vitamin C uptake
No full textVitamin C is accumulated in mammalian cells by two types of proteins: sodium-ascorbate co-transporters (SVCTs) and hexose transporters (GLUTs); in particular, SVCTs actively import ascorbate, the reduced form of this vitamin. SVCTs are surface glycoproteins encoded by two different genes, very similar in structure. They show distinct tissue distribution and functional characteristics, which indicate different physiological roles. SVCT1 is involved in whole-body homeostasis of vitamin C, while SVCT2 protects metabolically active cells against oxidative stress. Regulation at mRNA or protein level may serve for preferential accumulation of ascorbic acid at sites where it is needed. This review will summarize the present knowledge on structure, function and regulation of the SVCT transporters. Understanding the physiological role of SVCT1 and SVCT2 may lead to develop new therapeutic strategies to control intracellular vitamin C content or to promote tissue-specific delivery of vitamin C-drug conjugates. © 2007 Springer-Verlag
A sensitive SDS-page method separating myosin heavy chain isoforms of rat skeletal muscles reveals the heterogeneous nature of the embryonic myosin
No full textMyosin isoforms are used as markers of heterogeneity and plasticity of skeletal muscle fibers and motor units. Tedious and time-consuming methods, needing microgram or milligram amounts of myosin are widely used to characterize the heavy subunits. We here describe a sensitive method that separates in nanogram or microgram amounts the heavy chains of immature, fast and slow adult rat muscles in complex mixtures of myosins. Though the method is assembled from published procedures (SDS-PAGE, peptide mapping in the presence of SDS, silver stain) for the logical extensions introduced the end-product is a powerful tool to separate and characterize these high molecular weight biopolymers until now inseparable from complex mixtures. The method reveals the heterogeneous nature of the embryonic myosin heavy chains. © 1983 Academic Press, Inc
Kindesmissbrauch und Reviktimisierung
No full textLanger L, Catani C. Kindesmissbrauch und Reviktimisierung. Zeitschrift für Klinische Psychologie und Psychotherapie. 2017;45(4):279-289
Macrophages regulate proliferation and differentiation of satellite cells
No full textBiochem Biophys Res Commun. 1994 Aug 15;202(3):1688-96.
Macrophages regulate proliferation and differentiation of satellite cells.
Cantini M, Massimino ML, Bruson A, Catani C, Dalla Libera L, Carraro U.
Source
Department of Biomedical Sciences, University of Padova, Italy.
Abstract
We used an in vitro model to investigate whether macrophages stimulate satellite cells proliferation. Satellite cells were obtained by tryptic digestion of adult muscle. Macrophages were obtained from peritoneal cavity by wash after injection of thioglycolate broth. Macrophages and satellite cells cocultures showed an increased number of differentiated myotubes as compared to control cultures. Moreover, in conditions of myoblast colony growth, the addition of macrophage-conditioned medium resulted in a greater number of muscle cell colonies, which are richer in large and differentiated myotubes. The experiments with macrophage-conditioned media suggest that the increased muscle cell proliferation and differentiation is mediated by soluble factor(s) released by macrophages. These results demonstrate that besides their scavenger role macrophages play a pivotal role in myoblast proliferation during muscle regeneration.
PMID:
8060358
[PubMed - indexed for MEDLINE
Archaeological and historical notes on the Castellum Tariona in the Roman Era
No full textAutor donosi ispravak dosadašnjeg čitanja rimskoga
terminacijskog natpisa iz Bljušćevice kod sela Rastovca u
Trogirskoj zagori koji je u vrijeme Publija Kornelija Dolabele
uklesan u kamen živac. Također donosi novi nalaz cipusa iz zaselka
Bašelovici u Grebaštici kod Šibenika. Na njemu se spominje
Salto(ru)m F(inis) / Tariot(arum), a oznacava zapadnu granicu
tariotskog pašnjaka.The author provides a correction to the previous reading of the
Roman boundary inscription from Bljušćevica near the village of
Rastovac in the elevated hinterland of Trogir which was engraved
in solid rock during the consulship of Publius Cornelius Dolabella.
He also presents a new discovery of a cippus from the hamlet
of Bašelovici in Grebaštica near Šibenik. The inscription reads
Salto(ru)m F(inis) / Tariot(arum), designating the western boundary
of the Tariote pastures
Effects of beta 1-integrin antisense phosphorothioate-modified oligonucleotide on myoblast behaviour in vitro.
No full textCell Biochem Funct. 1995 Jun;13(2):99-104.
Effects of beta 1-integrin antisense phosphorothioate-modified oligonucleotide on myoblast behaviour in vitro.
Carraro U, Bruson A, Catani C, Dalla Libera L, Massimino ML, Rizzi C, Rossini K, Sandri M, Cantini M.
Source
University of Padova, CNR Unit for Muscle Biology and Physiopathology, Department of Biomedical Sciences, Italy.
Abstract
Myoblasts gene-engineered in vitro and then injected in vivo are safe, efficient options for gene therapy. While isolation of satellite cells is routinely achieved, their proliferation potential in vitro remains a limiting factor for cell transplantation under clinical conditions. We have studied the role of reversible inhibition of gene expression by antisense oligonucleotides on the proliferation of the myogenic cells. Addition of antisense oligonucleotides to myoblast cultures has been used to inhibit specifically the expression of the beta 1-integrin subunit gene. Here we show that the effects of multiple pulses of a phosphorothioate oligodeoxinucleotide antisense on the attachment to substrata and on the proliferation of myoblasts are dose-dependent. The addition of antisense to rat myoblasts caused rounding up of the cells and most of the cells became detached after several days in culture. A single pulse did not show any consistent effect, while in the presence of continuously administered antisense, the relative numbers of myoblasts in the treated muscle culture increased. We have no evidence of inhibition of myoblast fusion under these conditions. On the other hand, [3H]-TdR incorporation, total DNA and total number of cells decreased in antisense-treated cultures thus demonstrating an inhibitory effect of the phosphorothioate oligonucleotides on DNA synthesis. These side-effects could be overcome by substituting the phosphorothioate by unmodified oligonucleotides, so decreasing the half-life of the antisense, but also its toxicity. The overall results suggest a potential role of integrin antisense strategy in modulating the potential of myoblasts to proliferate.
PMID:
7538914
[PubMed - indexed for MEDLINE
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