323 research outputs found
Resilient agrarian landscapes in face of changes: the coevolutive approach to understand the links between communities and environmental characters
No full textRole of human tissue kallikrein in gastrointestinal stromal tumour invasion
No full textBackground:
Human tissue kallikrein (hK1) generates vasodilator kinins from kininogen and promotes angiogenesis by kinin-dependent and kinin-independent mechanisms. Here, we investigate the expression and functional relevance of hK1 in human gastrointestinal stromal tumour (GIST).<p></p>
Methods:
Vascularisation and hK1 expression of GIST samples were assessed by immunohistochemistry. In two GIST cell lines, hK1 expression was assessed by PCR, and hK1 protein levels and activity were measured by ELISA and an amidolytic assay, respectively. The effect of hK1 silencing, inhibition or overexpression on GIST cell proliferation, migration and paracrine induction of angiogenesis was studied. Finally, local and systemic levels of hK1 were assessed in mice injected with GIST cells.<p></p>
Results:
Human tissue kallikrein was detected in 19 out of 22 human GIST samples. Moreover, GIST cells express and secrete active hK1. Titration of hK1 demonstrated its involvement in GIST invasive behaviour, but not proliferation. Furthermore, hK1 released by GIST cells promoted endothelial cell migration and network formation through kinin-dependent mechanisms. Gastrointestinal stromal tumour implantation in nude mice resulted in local and systemic hK1 expression proportional to tumour dimension.<p></p>
Conclusions:
Human tissue kallikrein is produced and released by GIST and participates in tumour invasion. Further studies are needed to validate hK1 as a diagnostic biomarker and therapeutic target in GIST
Studies of the cardiovascular effects of nociceptin and related peptides
No full textNociceptin, a novel opioid peptide, and its ORL1 receptor share structural similarities with other opioid ligands and receptors. Although
NC exerts evident cardiovascular effects at a central and peripheral level, its role in homeostatic mechanisms and disease states are just
beginning to be understood, as only recently selective receptor antagonists became available. In this review, some of the new observations
regarding the cardiovascular actions of NC, related peptides and newly synthesized receptor antagonists are discussed
Arcispedale Sant'Anna: un ospedale dei bambini per Ferrara
No full textRicerca su possibili ipotesi di recupero del complesso ospedaliero ferrarese come centro regionale per la cura dei bambini. Sono studiati aspetti della progettazione dedicata all'ospedalizzazone dei bambini in realtà maggiormente innovative del nostro Paese. È analizzato il problema dell'umanizzazione negli ospedali dell'Emilia Romagna come base per sviluppare linee di indirizzo metaprogettuali
I progetti
No full textPresentazione dei risultati del workshop Sealine | Brindisi e riflessioni sul futuro del paesaggio portual
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