20 research outputs found
Dielectrophoresis: A Review of Applications for Stem Cell Research
Get PDFDielectrophoresis can discriminate distinct cellular identities in heterogeneous populations, and monitor cell state changes associated with activation and clonal expansion, apoptosis, and necrosis, without the need for biochemical labels. Demonstrated capabilities include the enrichment of haematopoetic stem cells from bone marrow and peripheral blood, and adult stem cells from adipose tissue. Recent research suggests that this technique can predict the ultimate fate of neural stem cells after differentiation before the appearance of specific cell-surface proteins. This review summarises the properties of cells that contribute to their dielectrophoretic behaviour, and their relevance to stem cell research and translational applications.</p
Human IgG1 antibodies suppress angiogenesis in a target-independent manner.
No full textberrant angiogenesis is implicated in diseases affecting nearly 10% of the world's population. The most widely used anti-angiogenic drug is bevacizumab, a humanized IgG1 monoclonal antibody that targets human VEGFA. Although bevacizumab does not recognize mouse Vegfa, it inhibits angiogenesis in mice. Here we show bevacizumab suppressed angiogenesis in three mouse models not via Vegfa blockade but rather Fc-mediated signaling through FcγRI (CD64) and c-Cbl, impairing macrophage migration. Other approved humanized or human IgG1 antibodies without mouse targets (adalimumab, alemtuzumab, ofatumumab, omalizumab, palivizumab and tocilizumab), mouse IgG2a, and overexpression of human IgG1-Fc or mouse IgG2a-Fc, also inhibited angiogenesis in wild-type and FcγR humanized mice. This anti-angiogenic effect was abolished by Fcgr1 ablation or knockdown, Fc cleavage, IgG-Fc inhibition, disruption of Fc-FcγR interaction, or elimination of FcRγ-initated signaling. Furthermore, bevacizumab's Fc region potentiated its anti-angiogenic activity in humanized VEGFA mice. Finally, mice deficient in FcγRI exhibited increased developmental and pathological angiogenesis. These findings reveal an unexpected anti-angiogenic function for FcγRI and a potentially concerning off-target effect of hIgG1 therapies
Author Correction: Comparison of CTX-M encoding plasmids present during the early phase of the ESBL pandemic in western Sweden
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Investigations on Flow Characteristics of Mortars Using Partial Replacement of Fine Aggregates with Processed Granulated Blast Furnace Slag
No full textAssessment on Performance of Steel Slag and Processed Granulated Blast Furnace Slag as an Alternative for Fine Aggregate—An Assertive Review
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Manufacturing and Characterization of Hybrid Composites with Basalt and Flax Fabrics and a Partially Bio-based Epoxy Resin
Get PDF[EN] This research is focused on manufacturing and characterization of hybrid composite laminates obtained different stacking sequences of basalt and flax fabrics with silane treatments embedded in a partially bio-sourced epoxy resin as matrix. They were manufactured by the vacuum-assisted resin infusion molding and mechanical properties were tested in tensile, flexural and impact conditions. The effect of the coupling agent on the fiber/matrix interface was studied by FESEM. The effect of temperature on mechanical properties was evaluated by DMTA and TMA. FESEM images revealed improved fiber/matrix interactions with silane treatment, having a more satisfactory effect on basalt fibers than on flax fibers because of its silica-based structure, leading to improved mechanical properties. It is worthy to note that the hybrid stacking sequence has no remarkable influence on the elongation at break. On the contrary, the hybrid stacking sequence offered a great influence on both the elastic modulus and the tensile strength.This research was funded by the Ministerio de Economía, Industria y Competitividad (MICINN) project number MAT2017-84909-C2-2-R. D. Lascano wants to thank UPV for the grant received though the PAID-01-18 program. Microscopy services at UPV are acknowledged for their help in collecting and analyzing FESEM images.Lascano-Aimacaña, DS.; Balart, R.; Garcia-Sanoguera, D.; Agüero-Rodríguez, Á.; Boronat, T.; Montanes, N. (2021). Manufacturing and Characterization of Hybrid Composites with Basalt and Flax Fabrics and a Partially Bio-based Epoxy Resin. Fibers and Polymers. 22(3):751-763. https://doi.org/10.1007/s12221-021-0209-5S751763223S. Yang, V. B. Chalivendra, and Y. K. Kim, Compos. Struct., 168, 120 (2017).R. Rahman and S. Z. F. S. 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Influence of Superplasticizers on Blended Cement and Their Effect on Flow Characteristics by Incorporating PGBS as Partial Replacement for Fine Aggregates
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