103 research outputs found
Characterisation of selected enzymes and regulators involved in flavonoid biosynthesis in banana and rapeseed
No full textBusche M. Characterisation of selected enzymes and regulators involved in flavonoid biosynthesis in banana and rapeseed. Bielefeld: Universität Bielefeld; 2022
Supplemental Material for Busche et al., 2019
No full textBusche M, Pucker B, Viehöver P, Weisshaar B, Stracke R. Supplemental Material for Busche et al., 2019. GSA Journals; 2019.Version 2
File S1. Per chromosome read coverage distribution of Dwarf Cavendish reads.
File S2. Comparison of SNVs in the duplicated segment on the long arm of chromosome 2 to all other SNVs in the genome. The higher read coverage at variants indicates a duplication of this region.
File S3. List of public genomic banana sequence read samples used for comparison against Dwarf Cavendish based on the DH Pahang reference.
File S4. Coverage plots of public genomic banana sequence read samples (File S1) for comparison against Dwarf Cavendish based on the DH Pahang reference. Samples are: Musa acuminata, Musa acuminata AYP_BOSN_r1, Musa acuminata ssp. banksii, Musa acuminata ssp. burmannica, Musa acuminata Cavendish BaXiJiao, Musa acuminata Gros Michel, Musa acuminata ssp. malaccensis, Musa acuminata Sucrier (Pisang Mas), Musa acuminata Sucrier (Pisang Mas 1998-2307), Musa acuminata ssp. zebrina (blood banana), Musa balbisiana Pisang Klutuk Wulung, Musa itinerans, Musa schizocarpa.
File S5. List of selected high impact variants between Dwarf Cavendish and DH Pahang with resulting effects predicted by SnpEff.</br
Digestive Enzymes of Human and Nonhuman Primates
No full textAll living organisms need to consume nutrients to grow, survive, and reproduce, making the successful acquisition of food resources a powerful selective pressure. However, acquiring food is only part of the challenge. While all animals spend much of their daily activity budget hunting, searching for, or otherwise procuring food, a large part of what is involved in overall nutrition occurs once the meal has been swallowed. Most nutritional components are too complex for immediate use and must be broken down into simpler compounds, which can then be absorbed by the body. This process, digestion, is catalyzed by enzymes that are either endogenous or produced by the host's microbial population. Research shows that the nutritional value of food is partially constrained by the digestive abilities of the microbial community present in the host's gut and that these microbes rapidly adapt to changes in diet and other environmental pressures. An accumulating body of evidence suggests that endogenously produced digestive enzymes also have been, and still are, common targets of natural selection, further cementing their crucial role in an organism's digestive system.Peer reviewe
Supplemental Material for Busche et al., 2019
No full textFile S1. Per chromosome read coverage distribution of Dwarf Cavendish reads.
File S2. Comparison of SNVs in the duplicated segment on the long arm of
chromosome 2 to all other SNVs in the genome. The higher read coverage at
variants indicates a duplication of this region.
File S3. List of public genomic banana sequence read samples used for
comparison against Dwarf Cavendish based on the DH Pahang reference.
File S4. Coverage plots of public genomic banana sequence read samples (File
S1) for comparison against Dwarf Cavendish based on the DH Pahang reference.
Samples are: Musa acuminata, Musa
acuminata AYP_BOSN_r1, Musa acuminata ssp. banksii, Musa acuminata ssp. burmannica, Musa acuminata Cavendish BaXiJiao, Musa
acuminata Gros Michel, Musa acuminata ssp. malaccensis, Musa
acuminata Sucrier (Pisang Mas), Musa
acuminata Sucrier (Pisang Mas 1998-2307), Musa acuminata ssp. zebrina
(blood banana), Musa balbisiana
Pisang Klutuk Wulung, Musa itinerans, Musa schizocarpa.
File S5. List of selected high impact variants between Dwarf Cavendish and DH
Pahang with resulting effects predicted by SnpEff.
</p
Genome assemblies of Musa acuminata Dwarf Cavendish
No full textPucker B, Busche M, Viehöver P, Weisshaar B, Stracke R. Genome assemblies of Musa acuminata Dwarf Cavendish. Bielefeld University; 2019.Please see https://doi.org/10.1101/691923 for a description of the data set and applied methods
Item 5 : RDM Workshop
No full textThe workshop focused on strengthening the library staff's competencies in Research Data Management (RDM)
The aim was to motivate the library staff to take ownership of skills development opportunities within this area of Open Science.
Library staff who attended this workshop can actively participate in a strengthened service of the researchers at their local institutions. To achieve this, the workshop introduced the research process based on the research project life cycle (RPLC) and gave perspective to a Research Data Life Cycle. This included an introduction to the FAIR principles and Data Management Plans (DMP). The workshop had a practical approach to RDM and therefore introduced two cases from, respectively the humanities and science, as examples of working with DMP in various disciplines.The teaching method of the workshop consisted of a mix of lectures and hands-on activities.
The workshop programme and learning objectives are in Danish, however the workshop material, presentations and further readings are in English.
File 5.1 [in Danish]: Programme and learning objective
Authors: Mareike C. H. Buss, Haakon Lund, Katrine Flindt Holmstrand
File 5.2: What is Open Science and the library role
Author: Katrine Flindt Holmstrand
File 5.3: Research Data Life Cycle
Author: Haakon Lund
File 5.4: FAIR Principles
Author: Mareike C.H. Buss
File 5.5: Case from Humanities
Author: Lene Offersgaard
File 5.6: Case from Natural Sciences
Author: Falco J. Hüser
File 5.7: Inspiration to further reading/training
Author: Mareike C.H. Buss, Katrine Flindt Holmstrand
File 5.8: Workshop handout
Small sequence variants between the Musa acuminata cultivars Dwarf Cavendish and DH Pahang
No full textPucker B, Busche M, Viehöver P, Weisshaar B, Stracke R. Small sequence variants between the Musa acuminata cultivars Dwarf Cavendish and DH Pahang. Bielefeld University; 2019.Please see https://doi.org/10.1101/691923 for a description of the data set and applied methods
Additional file 1 of Generation and characterisation of an Arabidopsis thaliana f3h/fls1/ans triple mutant that accumulates eriodictyol derivatives
No full textSchilbert HM, Busche M, Sáez V, et al. Additional file 1 of Generation and characterisation of an Arabidopsis thaliana f3h/fls1/ans triple mutant that accumulates eriodictyol derivatives. figshare; 2024
Data_Sheet_1_Functional Characterisation of Banana (Musa spp.) 2-Oxoglutarate-Dependent Dioxygenases Involved in Flavonoid Biosynthesis.docx
Get PDFBananas (Musa) are non-grass, monocotyledonous, perennial plants that are well known for their edible fruits. Their cultivation provides food security and employment opportunities in many countries. Banana fruits contain high levels of minerals and phytochemicals, including flavonoids, which are beneficial for human nutrition. To broaden the knowledge on flavonoid biosynthesis in this major crop plant, we aimed to identify and functionally characterise selected structural genes encoding 2-oxoglutarate-dependent dioxygenases, involved in the formation of the flavonoid aglycon. Musa candidates genes predicted to encode flavanone 3-hydroxylase (F3H), flavonol synthase (FLS) and anthocyanidin synthase (ANS) were assayed. Enzymatic functionalities of the recombinant proteins were confirmed in vivo using bioconversion assays. Moreover, transgenic analyses in corresponding Arabidopsis thaliana mutants showed that MusaF3H, MusaFLS and MusaANS were able to complement the respective loss-of-function phenotypes, thus verifying functionality of the enzymes in planta. Knowledge gained from this work provides a new aspect for further research towards genetic engineering of flavonoid biosynthesis in banana fruits to increase their antioxidant activity and nutritional value.</p
De novo ONT long read assembly of the A. thaliana f3h/fls1/ans triple mutant
No full textSchilbert H, Busche M, Viehöver P, Weisshaar B, Stracke R. De novo ONT long read assembly of the A. thaliana f3h/fls1/ans triple mutant . Bielefeld University; 2023.We sequenced the genome of the Arabidopsis thaliana f3h/fls1/ans triple mutant (f3h (tt6-2), Col-0 background; fls1 (fls1-2), Nö-0 background; ans (tds4-4), Col-0 background) to fully characterise its insertion alleles with Oxford Nanopore Technologies (ONT) long reads. The DNA that has been sequenced was isolated form rosette leaves. Sequencing was performed on a R9.4.1 flow cell and Basecalling was carried out with Guppy v.4.2.3. The reads were assembled with Canu v2.1.1 and the assembly is available as individual FASTA file
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