3,138 research outputs found
Progetto Parco-Rifugio
Get PDFIl volume raccoglie i risultati degli studenti del Corso di Studi Architettura, DPIA, Università degli studi di Udine, che hanno partecipato ad un workshop indirizzato a produrre spunti di riflessione e idee di progetto per il parco del nuovo Parco Rifugio della Sezione di Udine dell’Ente Nazionale Protezione Animali - E.N.P.A. - in fase di realizzazione con la donazione della Fondazione F&F Foundation in memoria di Franco Faccin
Progettazione tecnica, funzioni e tipologie
No full textIl volume raccoglie i risultati degli studenti del Corso di Studi Architettura, DPIA, Università degli studi di Udine, che hanno partecipato ad un workshop indirizzato a produrre spunti di riflessione e idee di progetto per il parco del nuovo Parco Rifugio della Sezione di Udine dell’Ente Nazionale Protezione Animali - ENPA - in fase di realizzazione con la donazione della Fondazione F&F Foundation in memoria di Franco Faccin
Design, synthesis and biological characterization of PfGAPDH inhibitors
No full textMalaria has a tremendous health, social and economic impact on people living in tropical and subtropical regions of the world. WHO estimated 214 million cases and 438 000 deaths in 2015 and 3.2 billion people are globally at risk of infection1. Available treatments are becoming progressively less effective, mainly because parasites are constantly acquiring resistance toward the drug in use. Therefore, there is an urgent need of developing therapeutic agents acting on new targets.
Plasmodium parasites (P. falciparum is the most lethal), in their amastigote phase, produce energy only through glycolysis. In this pathway, GAPDH catalyses the first energy productive step and thus represent a valuable target for drug discovery. Known GAPDH inhibitors that bind to the active site are small nonselective molecules (iodoacetamide, 3-bromopyruvate), which irreversibly react with cysteine residues of the enzyme, including the catalytic one. We designed and synthesized the first series of inhibitors characterized by a 3-bromoisoxazoline warhead2, inspired by 3-bromoacivicin, a known inhibitor of reactive cysteine containing enzymes3. The compounds were assayed for the inhibitory activity on the P. falciparum isolated enzyme showing a biphasic irreversible inactivation. MS/MS studies of the digested protein demonstrated the mild reactivity of the exploited warhead, which only reacts with catalytic Cys (activated by a His residues). The human ortholog of GAPDH is only partially inhibited (up to 25%). We explained this through a negative cooperativity that prevents the alkylation of all the four monomers of hGAPDH, resulting in selectivity among the two isoforms of GAPDH4. Compounds were also assayed on cultures of the bloodstream form of P. falciparum and human cell lines in order to determine their in vitro antiparasitic activity and toxicity.
The goal of this project is to obtain compounds with improved potency and pharmacokinetic properties, suitable for in vivo studies on animal model of P. falciparum infections.
1. http://www.who.int/mediacentre/factsheets/fs094/en/
2. Bruno, S.; Pinto, A.; Paredi, G.; Tamborini, L.; De Micheli, C.; La Petra, V.; Marinelli, L.; Novellino, E.; Conti, P.; Mozzarelli, A. J. Med. Chem. 2014, 57(17), 7465-7471.
3. a) Conti, P.; Pinto, A.; Wong, P.E.; Major, L.L.; Tamborini, L.; Iannuzzi M.C.; De Micheli, C.; Barrett, M.P.; Smith, T.K. ChemMedChem 2011, 6(2), 329-333. b) Tamborini, L.; Pinto, A.; Smith, T.K.; Major, L.L.; Iannuzzi, M.C.; Cosconati, S.; Marinelli, L.; Novellino, E.; Lo Presti, L.; Wong, P.E.; Barrett, M.P.; De Micheli, C.; Conti, P. ChemMedChem 2012, 7(9), 1623-1634.
4. Bruno, S.; Margiotta, M.; Pinto, A.; Cullia, G.; Conti, P.; De Micheli, C.; Mozzarelli, A. Bioorg. Med. Chem. 2016, 24(12), 2654-2659
SHeLA: Scalable Heterogeneous Layered Attestation
Get PDFsponsorship: This work was supported in part by EU LOCARD Project under Grant H2020-SU-SEC-2018-832735, in part by the Central Europe Leuven Strategic Alliance under Grant CELSA/17/033, and in part by the Flemish Government under Grant G0E0719N. The work of M. M. Rabbani was supported by Fondazione Bruno Kessler Fund. The work of M. Conti was supported by the Marie Curie Fellowship through European Commission under Agreement PCIG11-GA-2012-321980. (Corresponding author: Md Masoom Rabbani.) (EU LOCARD Project under Grant H2020-SU-SEC-2018|832735, Central Europe Leuven Strategic Alliance|CELSA/17/033, Flemish Government|G0E0719N, Fondazione Bruno Kessler Fund, Marie Curie Fellowship through European Commission|PCIG11-GA-2012-321980)status: Publishe
The radical right in Europe, between slogans and voting behavior. IHS Political Science Series No. 123, July 2011
Get PDFThe paper analyzes the radical right‘s attitudes toward the EU focusing in particular on the level of congruence between the programmatic statements of the central office and the voting behavior of their MEPs. It shows that although radical right parties represent a source of opposition to the EU, within the EP they express their dissent making use of the rules of the game, voting with the opposition more than the other forces do, but voting almost as much with the majority. The party public office in the EP is inserted in the legislative process and even more collusive with the other parties of both sides of the political spectrum than the Eurosceptical rhetoric and statements of central office makes the public believe
Giancarlo ABBAMONTE, Ferruccio CONTI BIZZARRO, Luigi SPINA (a cura di), L'ultima parola. L'analisi dei testi : teorie e pratiche nell' Antichità greca e latina.
No full textRochette Bruno. Giancarlo ABBAMONTE, Ferruccio CONTI BIZZARRO, Luigi SPINA (a cura di), L'ultima parola. L'analisi dei testi : teorie e pratiche nell' Antichità greca e latina. . In: L'antiquité classique, Tome 75, 2006. pp. 391-392
Giancarlo ABBAMONTE, Ferruccio CONTI BIZZARRO, Luigi SPINA (a cura di), L'ultima parola. L'analisi dei testi : teorie e pratiche nell' Antichità greca e latina.
No full textRochette Bruno. Giancarlo ABBAMONTE, Ferruccio CONTI BIZZARRO, Luigi SPINA (a cura di), L'ultima parola. L'analisi dei testi : teorie e pratiche nell' Antichità greca e latina. . In: L'antiquité classique, Tome 75, 2006. pp. 391-392
Solid Oxide Fuel Cells: Numerical and Experimental Approaches
No full textSolid oxide fuel cell (SOFC) is a promising electrochemical technology that can produce electrical and thermal power with outstanding efficiencies. A systematic synergetic approach between experimental measurements and modelling theory has proved to be instrumental to evaluate performance and correct behaviour of a chemical process, like the ones occurring in SOFC. For this purpose, starting from SIMFC (SIMulation of Fuel Cells) code set-up by PERT-UNIGE (Process Engineering Research Group) for Molten Carbonate Fuel Cells [1], a new code has been set-up for SOFCs based on local mass, energy, charge and momentum balances. This code takes into account the proper reactions occurring in the SOFC as well as new geometries and kinetics thanks to experiments carried out on single cells and stack in ENEA laboratories of C.R. Casaccia and VTT Fuel Cell Lab in Finland. In particular using an innovative experimental setup it has been possible to study experimentally the influence of a multicomponent mixtures on the performance of SOFC and also validate locally a 2-D model developed starting from SIMFC code. The results obtained are good, showing a good agreement between experimental and numerical results. The obtained results are encouraging further studies which allow the model validation on a greater quantity of data and under a wider range of operating conditions
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