383 research outputs found
A DNA vaccine encoding ubiquitinated Rift Valley fever virus nucleoprotein provides consistent immunity and protects IFNAR−/− mice upon lethal virus challenge
No full textCurrent vaccine candidates against Rift Valley fever virus (RVFV) incorporate the viral structural glycoproteins as antigens, since triggering antibody responses against them usually correlates with protection. Here, we have focused solely on the nucleoprotein of RVFV as a potential target for vaccine development. Previous studies in mouse models have already demonstrated that RVFV nucleoprotein can elicit partial protection when administered by means of a DNA vaccine or in recombinant, soluble, protein form. To determine whether this partially protective immune response could be augmented to a level comparable to DNA constructs encoding for RVFV glycoproteins, several targeting sequences were cloned adjacent to the RVFV nucleoprotein (N) gene. Immunization with a plasmid construct encoding for a ubiquitinated form of the viral nucleoprotein (pCMV-Ub-N) significantly increased the survival of IFNAR-/- mice following viral challenge to levels comparable with a recombinant DNA-vaccine encoding both RVFV glycoproteins. Mice immunized with pCMV-Ub-N also displayed higher levels of non-neutralizing anti-N antibodies and antigen-specific T-cell responses. This suggests a role for other cell mediated responses in protection against RVFV. These findings show the potential of RVFV N as a candidate antigen for vaccination, and present a new strategy in vaccine design against certain bunyaviruses, where glycoprotein variation may impede effective broad-based vaccination strategies. © 2011 Elsevier Ltd
Priming with DNA plasmids encoding the nucleocapsid protein and glycoprotein precursors from Rift Valley fever virus accelerates the immune responses induced by an attenuated vaccine in sheep
No full textIn this work we tested the ability of plasmid DNA constructs encoding structural Rift Valley fever virus (RVFV) antigens to induce specific immune responses in sheep. The sole immunization of DNA constructs encoding the glycoprotein precursor NSm/G2/G1 did not suffice to induce a detectable antibody response. In contrast, immunization of sheep with a plasmid vector encoding the viral nucleocapsid protein N elicited a potent and long lasting induction of antibodies but with low neutralizing titers. After DNA immunization, no antigen-specific proliferating cells were detected in sheep PBLs. Boosting with the attenuated vaccine strain MP12 was able to increase the levels of proliferating memory cell pools and induction of IFN-γ in response to purified virus or recombinant proteins, particularly in sheep vaccinated with a combination of both plasmid constructs. These results open the possibility to exploit this strategy to improve the induction of immune responses against RVFV in sheep. © 2008 Elsevier Ltd. All rights reserved
In times of “crisis”: Puerto Rican activism, gender, and belonging in Orlando
No full textIn Times of “Crisis” is an ethnography of Puerto Rican activism in the Orlando metropolitan area that directly addresses contemporary events or moments of “crisis,” such as the fiscal crisis in Puerto Rico, the shooting at Pulse nightclub, the 2016 U.S. elections, and Hurricane Maria. Based on 24 months of ethnographic fieldwork conducted between 2016 and 2018, this dissertation asks and answers the following questions: What does the concept of crisis obscure and what knowledges does it make visible? How do Puerto Rican activists respond to these so-called “crises” in their own lives and communities? And how have conditions in Puerto Rico shaped activism in the diaspora to Orlando? Ultimately, this study of Puerto Rican activism argues for an understanding of the concept of crisis that does not denote exception, but rather continuities of social injustices across transnational spheres. This understanding also considers the ways that crisis is generative, producing alternative imaginings of a more just world and transnational spaces of belonging forged out of resistance.
The first full-length ethnographic study of Puerto Rican activism in Central Florida, this dissertation departs from traditional studies of social activism by examining how crisis and activism are interconnected across the borders of the nation-state. In the process, it demonstrates how the socio-economic and political climate in Puerto Rico has led to the crystallization of a particularly local Puerto Rican activist community in Orlando that imagines itself as part of a larger transnational Puerto Rican community. Further, by foregrounding the narratives of Puerto Rican women this work is positioned within a genealogy of women of color feminisms that attends to how marginalized populations interpret and contest intersecting forms of oppression.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2022-05-01The student, Julie Torres, accepted the attached license on 2020-05-06 at 21:29.The student, Julie Torres, submitted this Dissertation for approval on 2020-05-06 at 21:46.This Dissertation was approved for publication on 2020-05-07 at 14:32.DSpace SAF Submission Ingestion Package generated from Vireo submission #15263 on 2020-08-25 at 17:43:44Made available in DSpace on 2020-08-27T00:51:29Z (GMT). No. of bitstreams: 2
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A critical assessment of the theology of Camilo Torres in the light of Latin American theology : a theological paradigm for peace with justice for Colombia
No full textThe thesis aims to provide a critical assessment of Camilo Torres’ concept of efficacious love in the light of a hermeneutics and ethics of liberation, to contribute theoretically to theological reflection upon the mission of the Protestant and Roman Catholic churches in Colombia, and generally in Latin America. It proposes the thesis that the theology of Camilo Torres, viewed from the perspective of a hermeneutics and an ethics of liberation, can be foundational for seeking, constructing, and sustaining peace with justice in a context of oppression and violence.
Based on the theological analysis, the academic and spiritual motivation should respond to two fundamental questions in our academic inquiry: what foundation exists in Camilo Torres’ theology for the construction of a just, peaceful, liberative society and to enable proximity to the excluded, victimized, and poor population in Colombia to be achieved? And how can we as Christians respond to the grace of God in living efficaciously the values of the Kingdom of God, in order to bring structural changes in Colombia? The implications of the answers to those questions would result in the possibility to execute an integrating theological proposal for peace with justice for the church in Colombia.
In an interpretative mode, we consider critically the multidisciplinary interaction of some of the theological foundations of liberation theology. Our task consists in clarifying and constructing theological presuppositions for a dialectical examination of the historical and current situation in Colombia, viewed in the light of the internal problems and realities. Thus, it is our intention in order to attempt a significant interdisciplinary juncture, to examine and interpret such relevant concepts as efficacious love, faith with works, kenosis, hypostatic union, hermeneutics and ethics of liberation, social justice, personal and collective socio-political conversion, and a praxis of faith as the church’s mission in response to the Colombian context of poverty and violence
Rift Valley Fever: Recent Insights into Pathogenesis and Prevention
No full textABSTRACT
Rift Valley fever virus (RVFV) is a zoonotic pathogen that primarily affects ruminants but can also be lethal in humans. A negative-stranded RNA virus of the family
Bunyaviridae
, this pathogen is transmitted mainly via mosquito vectors. RVFV has shown the ability to inflict significant damage to livestock and is also a threat to public health. While outbreaks have traditionally occurred in sub-Saharan Africa, recent outbreaks in the Middle East have raised awareness of the potential of this virus to spread to Europe, Asia, and the Americas. Although the virus was initially characterized almost 80 years ago, the only vaccine approved for widespread veterinary use is an attenuated strain that has been associated with significant pathogenic side effects. However, increased understanding of the molecular biology of the virus over the last few years has led to recent advances in vaccine design and has enabled the development of more-potent prophylactic measures to combat infection. In this review, we discuss several aspects of RVFV, with particular emphasis on the molecular components of the virus and their respective roles in pathogenesis and an overview of current vaccine candidates. Progress in understanding the epidemiology of Rift Valley fever has also enabled prediction of potential outbreaks well in advance, thus providing another tool to combat the physical and economic impact of this disease.
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Al grito de ¿guerra? Museo de la Muerte. Irán, exposición temporal. Máscara Ndeemba. 36 Tercera época (2005-2006) octubre-enero. Gaceta de Museos
No full text- La difusión en los quehaceres del museo - El Museo de la Muerte, por Elsa Malvido - Mexicanos al grito de ...¿guerra?, por Alejandro Cortés y Katia Teodocio - Máscara Ndeemba, por Raffela Cedraschi - Todas las voces: una experiencia radiofónica en Querétaro, por Claudia Pilar Dovali Torres - Museo del Estanquillo, por Alejandro Mendoza - Irán, una exposición temporal, por Alejandra Gómez Colordo - La seguridad en los museos, por Héctor Mendoza - La memoria sonora en voz de los protagonistas, por Gabriela Marentes - La conservación del disco compacto, por Axel Solórzano - La vitrina del mes: Museo Arqueológico Caxitlán - La cédula del mes: cédulas de objetos, una nota - El material educativo del mes: Los Artenautas visitan el Museo del Desierto - Noticias y reseñas
Análisis y técnica de manufactura del Códice Azoyú 2. Antropología. Boletín Oficial del Instituto Nacional de Antropología e Historia: Cien años. Anales del Museo Nacional de México (1877-1977). Num. 69 Nueva Época (2003) enero-marzo
No full textCarrillo y Gariel, Abelardo, Técnica de la pintura de la Nueva España, México, UNAM-Instituto de Investigaciones Estéticas, 1946, p. 35.De Gortari, Eli, Del saber y de la técnica del México antiguo, México, UNAM (Complementos del Seminario de Problemas Científicos y Filosóficos 3,nueva época), 1987, p. 49.Gettens, R. J. y G. L. Stout, Painting Materials. A Short Encyclopaedia, New York, Dover Publications Inc., 1966, p. 117.Glass, John, Catálogo de la Colección de Códices, México, INAH-Museo Nacional de Antropología, 1964, p. 165.Huerta Carrillo, Alejandro , “Análisis de materiales del Códice Azoyú 1”, en Constanza Vega Sosa, Códice Azoyú 1. El Reino de Tlachinolan, México, FCE, 1991, p. 128.Huerta Carrillo. Alejandro , “Análisis químico y técnica de manufactura del Códice Moctezuma”, inédito, p. 8.Huerta Carrillo, Alejandro , “Análisis de la policromía de los petroglifos de la Estructura A”, en Constanza Vega Sosa, El recinto sagrado de México-Tenochtitlan, México, SEP-INAH, 1979, pp. 87-94.Huerta Carrillo, Alejandro, “Estudio de la policromía de la Piedra de la Luna-Coyolxauhqui”, en Churubusco, México, INAH-Dirección de Restauración, 1977, p. 93.Huerta Carrillo, Alejandro, “Análisis de la pintura mural de la Zona Arqueológica de Palenque, Chiapas”, en Segundo Encuentro Nacional de Restauradores del Patrimonio Cultural, México, INAH-Dirección de Restauración del Patrimonio Cultural, 1983, p. 23.Huerta Carrillo, Alejandro, y Eugenia Berthier V., “Códices, la ciencia al rescate”, inédito, 1999, p. 8.Landa A., Ma. Elena et al., La Garrafa, México, Gobierno del Estado de Puebla / INAH/SEP, 1988, p. 244.Stromberg, Gobi (coord.), El Universo del Amate, México, SEP-Museo Nacional de Culturas Populares, 1982, pp. 13, 23.Torres Montes, Luis, “Materiales y técnica de la pintura mural de Teotihuacan”, en Teotihuacan, México, Sociedad Mexicana de Antropología, 1972, p. 23.Vega, Constanza, Códices y Documentos sobre México, México, INAH (Serie Historia), 1994, pp. 165-168
CD8 T Cell Responses to an Immunodominant Epitope within the Nonstructural Protein NS1 Provide Wide Immunoprotection against Bluetongue Virus in IFNAR <sup>−/−</sup> Mice
No full textConventional vaccines have controlled or limited BTV expansion in the past, but they cannot address the need for cross-protection among serotypes and do not allow distinguishing between infected and vaccinated animals (DIVA strategy). There is a need to develop universal vaccines that induce effective protection against multiple BTV serotypes. In this work we have shown the importance of the nonstructural protein NS1, conserved among all the BTV serotypes, in CD8 T cell-mediated protection against multiple BTV serotypes when vectorized as a recombinant MVA vaccine.</jats:p
Using RVFV as a Vector Platform for the Expression of Ruminant Disease Antigens
No full text209–225 ppLive attenuated viruses remain as vaccine agents with unparalleled performance in terms of duration, magnitude, and breadth of induced immune responses. As the yellow fever-attenuated vaccine strain Y17D, attenuated Rift Valley fever virus shares features suitable to be used as a viral vector for heterologous antigen expression and bivalent vaccine development. Current reverse genetics technology showed the successful rescue of RVFV carrying foreign antigens with little immunogenicity loss in experimental animal models. We show here the basic experimental protocol to achieve the expression of candidate vaccine antigens from other important diseases of ruminants using RVFV as a vector platform as well as preliminary steps for the characterization of immunogenicity in vivo.This work was funded by grants AGL2017-83226R and S2018/BAA-4370 from Ministerio de Ciencia and Comunidad de Madrid respectively.Peer reviewe
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