1,721,596 research outputs found
Aldo Messina. —Le chiese rupestri del Val di Noto. Palerme, Istit. sicil. studi bizant. e Neoellen., 1994 (Monumenti, 4)
No full textBozzoni Corrado. Aldo Messina. —Le chiese rupestri del Val di Noto. Palerme, Istit. sicil. studi bizant. e Neoellen., 1994 (Monumenti, 4). In: Cahiers de civilisation médiévale, 41e année (n°164), Octobre-décembre 1998. pp. 409-410
U1 small nuclear RNA chimeric ribozymes with substrate specificity for the Rev pre-mRNA of human immunodeficiency virus
No full textThe in vivo effectiveness of ribozymes strongly depends on the correct choice of the vector molecule. High levels of expression, stability, active conformation, and correct cellular localization are the most important features for a ribozyme vector. We have exploited the utilization of the U1 small nuclear RNA (snRNA) as a vector for specifically targeting a ribozyme into the nucleus. The Rev pre-mRNA of human immunodeficiency virus type 1 was chosen as target for testing the activity of the Ul-ribozyme. The catalytic core of the hammerhead motif, plus the recognition sequences, substituted the stem-loop III of the U1 snRNA. The resulting construct displays efficient cleavage activity in vitro. In addition, in the in vivo system of Xenopus laevis oocytes, the Ul-chimeric ribozyme accumulates in large amounts in the nucleus and produces a considerable reduction of Rev pre-mRNA levels. The Rev-specific ribozyme was also inserted in a derivative of the Ul snRNA mutated in the region of pairing with the 5' splice site, such as to match it with the suboptimal splice junction of the Rev precursor. This construct shows more efficient reduction of Rev pre-mRNA in vivo than the wild-type U1 vector
Corrado Bozzoni. — Calabria normanna: ricerche sull' 'architettura di secoli XI e XII, 1974 (" Saggi di stor. dell'architettura ", 1)
No full textHéliot Pierre. Corrado Bozzoni. — Calabria normanna: ricerche sull' 'architettura di secoli XI e XII, 1974 (" Saggi di stor. dell'architettura ", 1). In: Cahiers de civilisation médiévale, 20e année (n°80), Octobre-décembre 1977. pp. 357-359
Use of adenoviral VAI small RNA as a carrier for cytoplasmic delivery of ribozymes
No full textThe in vivo effectiveness of therapeutic RNAs, like antisense molecules and ribozymes, relies on several features: RNA molecules need to be expressed at high levels in the correct cellular compartment as stable and active molecules. The exploitation of "natural" small RNA coding genes as expressing cassettes gives high chances to fulfill these requirements. We have investigated the utilization of the adenoviral VAI RNA as a cytoplasmatic carrier for expressing ribozymes against HIV-1. The conserved 5' leader sequence of HIV was chosen as a target, because it is present in all the viral transcripts and is highly conserved. Hammerhead ribozymes were substituted to different portions of the VAI RNA and the resulting chimera were tested in the in vivo system of Xenopus laevis oocytes for their level of accumulation, cellular compartmentalization, and assembly in specific ribonucleoparticles containing the La antigen. Interesting differences in the activity of the different chimera were found in both in vitro cleavage assays and S100 extracts of injected oocytes where the catalytic activity of the ribozymes in the RNP context can be analyzed
Ricerca storico-architettonica sull'immobile sito a piazza Verdi in Roma - ex Istituto Poligrafico e Zecca dello Stato
No full textLa ricerca storico-documentaria archivistica, ha avuto l'obiettivo di ricostruire puntualmente le diverse fasi progettuali e soprattutto costruttive dell'edificio del Poligrafico e Zecca dello Stato di Piazza Verdi. particolare attenzione è stata rivolta alla comprensione della struttura statica e tecnico-costruttiva dell'ingente manufatto architettonico. La relazione finale ha messo in luce il valore storico-artistico dell'edificio considerandone le singole parti e le rispettive epoche di costruzione
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