39 research outputs found
Author Correction: Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores
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A Highly Selective Fluorescent Probe for Visualization of Organic Hydroperoxides in Living Cells
No full textThe transcriptional regulatory protein OhrR is converted into a fluorescent bioprobe capable of detecting organic hydroperoxides in living cells with high sensitivity and selectivity.Chemistry, MultidisciplinarySCI(E)EIPubMed15ARTICLE4817065-1706713
Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores
No full textAbstract 5-hydroxymethylcytosine (5 hmC), the oxidized form of 5-methylcytosine (5 mC), is a base modification with emerging importance in biology and disease. However, like most epigenetic elements, it is transparent to many conventional genetic techniques and is thus challenging to probe. Here, we report a rapid solid-state nanopore assay that is capable of resolving 5 hmC with high specificity and sensitivity and demonstrate its utility in assessing global modification abundance in genomic DNA
A Selective Fluorescent Probe for Carbon Monoxide Imaging in Living Cells
No full textA genetically encoded fluorescent probe is capable of selectively detecting carbon monoxide inside living cell. The probe, named COSer (CO sensor), consists of a circularly permuted yellow fluorescent protein (cpYFP) inserted into the regulatory domain of the bacterial CO-sensing protein, CooA, which gives the probe its selective CO-binding property. Copyright ? 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Chemistry, MultidisciplinarySCI(E)EI0ARTICLE389652-96565
