2,993 research outputs found
Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans
No full textThe protein DR_2577 is a major Surface layer component of the radio-resistant bacterium Deinococcus radiodurans. In the present study DR_2577 has been purified and its oligomeric profile characterized by means of size exclusion chromatography and gel electrophoresis. DR_2577 was found to be organized into three hierarchical orders characterized by monomers, stable dimers formed by the occurrence of disulfide bonds, and hexamers resulting from a combination of dimers. The structural implications of these findings are discussed providing new elements for a more integrated model of this S-layer
Raoult's law revisited: accurately predicting equilibrium relative humidity points for humidity control experiments
No full textThe humidity surrounding a sample is an important variable in scientific experiments. Biological samples in particular require not just a humid atmosphere but often a relative humidity (RH) that is in equilibrium with a
stabilizing solution required to maintain the sample in the same state during measurements. The controlled dehydration of macromolecular crystals can lead
to significant increases in crystal order, leading to higher diffraction quality. Devices that can accurately control the humidity surrounding crystals while
monitoring diffraction have led to this technique being increasingly adopted, as the experiments become easier and more reproducible. Matching the RH to the
mother liquor is the first step in allowing the stable mounting of a crystal. In previous work [Wheeler, Russi, Bowler & Bowler (2012). Acta Cryst. F68, 111–
114], the equilibrium RHs were measured for a range of concentrations of the most commonly used precipitants in macromolecular crystallography and it was shown how these related to Raoult’s law for the equilibrium vapour pressure of water above a solution. However, a discrepancy between the measured values and those predicted by theory could not be explained. Here, a more precise humidity control device has been used to determine equilibrium RH points. The new results are in agreement with Raoult’s law. A simple argument in statistical mechanics is also presented, demonstrating that the equilibrium vapour pressure of a solvent is proportional to its mole fraction in an ideal solution: Raoult’s law. The same argument can be extended to the case where the solvent and solute molecules are of different sizes, as is the case with polymers. The results provide a framework for the correct maintenance of the RH surrounding a sample
New features of the cell wall of the radio-resistant bacterium Deinococcus radiodurans
No full textWe have analyzed the cell wall of the radio-resistant bacterium Deinococcus radiodurans. Unexpectedly, the bacterial envelope appears to be organized in different complexes of high molecular weight. Each complex is composed of several proteins, most of which are coded by genes of unknown function and the majority are constituents of the inner/outer membrane system. One of the most abundant complexes is constituted by the gene DR_0774. This protein is a type of secretin which is a known subunit of the homo-oligomeric channel that represents the main bulk of the type IV piliation family. Finally, a minor component of the pink envelope consists of several inner-membrane proteins. The implications of these findings are discussed
Comparative feasibility study of a 30 MW disruptive floater solution with a 15 MW PivotBuoy and a benchmark 15 MW semi-submersible floater in the Bay of Biscay
No full textThis paper investigates the technical, life cycle, and economic feasibility of a 30 MW upscaled downwind turbine, comparing it to a 15 MW X1 Wind PivotBuoy downwind turbine and a benchmark 15 MW IEA Umaine VolturnUS-S upwind turbine in the 450 MW Sud de la Bretagne I wind farm site. The study is significant due to the rising energy demand, the potential for decreasing the levelized cost of energy with increased turbine size, and the optimized use of space. The size limit of current upwind turbine designs could be addressed using a downwind turbine solution.The research is conducted by modelling the global dynamic response of the structure using OpenFAST and computing the natural frequencies and stresses using a finite element model. A lifecycle analysis is performed to identify potential pitfalls and bottlenecks by analysing the individual lifecycle phases. The economic feasibility is assessed by simulating the annual energy production using TOPFARM and utilizing structural analysis and lifecycle assessment to quantify capital, operational, and abandonment expenditures. Based on the annual energy production and the performance indicators the levelized cost of energy is calculated.The findings indicate that while the global stability is within boundaries, the stress in members is too high with a simple scale-up of the proposed design. Bottlenecks are found in lifting operations and supply chain readiness. The levelized cost of energy and capital expenditure increased due to substructure self-weight, rendering the proposed 30 MW scale-up currently unfeasible when compared to the other two wind farms.These findings are important as they demonstrate that the 15 MW X1 Wind PivotBuoy is not scalable without design changes. The levelized cost of energy does not decrease with an increased floater solution. The 15 MW X1 Wind PivotBuoy downwind turbine seems more economically viable, making it a more interesting option for future development.Civil Engineerin
Short-circuit characteristics of superconducting permanent magnet generators for 10 MW wind turbines
No full textSuperconducting permanent magnet generators (SCPMGs) are a potential candidate for 10 MW direct-drive wind turbine applications. This paper presents two 10 MW SCPMG designs using MgB<sub>2</sub> cables for the armature winding and investigates the short-circuit characteristics of the designed SCPMGs. The first part of the results shows that the SCPMGs can double the shear stress of a conventional low-speed permanent magnet (PM) generator (from 65 kPa to 130 kPa) whilst avoiding demagnetization of the PMs in rated-load operation. However, the power factor has to drop to a range of 0.7-0.8. The second part of the results shows that during a sudden three-phase short circuit, the superconducting armature winding is prone to quench and the PMs are likely to be demagnetized in both proposed designs.Accepted Author ManuscriptTransport Engineering and Logistic
An Ultrasonically Powered System Using an AlN PMUT Receiver for Delivering Instantaneous mW-Range DC Power to Biomedical Implants
No full textAluminum Nitride (AlN) Piezoelectric Micromachined Ultrasonic Transducers (PMUTs) are gaining interest for biomedical implant power due to biocompatibility and lowtemperature processing. However, due to the low piezoelectric coefficient of AlN PMUTs, storage capacitors are often used to accumulate ultrasonic power transferred over an extended time. The accumulated energy is then used to power a DC load, which leads to a long start-up time, and insufficient duty cycle for some applications. We present an ultrasonically powered system for biomedical implants capable of delivering mW-range instantaneous power to DC loads, without pre-storing it. The system features a 25 mm2 AlN PMUT, an inductive matching network, and an application-specific power management integrated circuit(ASIC). For an acoustic intensity of 360 mW/cm2 at the surface of the PMUT, an open-circuit voltage of 1.11 V and an aperture efficiency of 30.5 % are measured. Furthermore, by connecting a series-matching inductor to the PMUT, the highest-reported power delivered to the load (PDL) of 6.4 mW is measured over an optimal load of 7.6 Ω. Finally, together with the ASIC and at the intensity of 108 mW/cm2, our system delivers 1.04 mW DC power to a 3.3 kΩ load, which is over two orders of magnitude higher than the previously reported average DC power for AlN PMUTs.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Bio-ElectronicsElectronic Components, Technology and Material
Inversion of coseismic deformation due to the 8th February 2016, Mw 4.2 earthquake at Los Humeros (Mexico) inferred from DInSAR
No full textOn the 8th of February 2016, a Mw 4.2 earthquake was detected inside the Los Humeros caldera, located in the eastern sector of the Trans-Mexican Volcanic Belt. The event occurred after a sharp increase in the injection rate at the Los Humeros Geothermal Field and it was recorded by the seismic monitoring network of the power plant. The earthquake was felt by the local population and it caused damage in the power plant infrastructure. The focal mechanism solution of a previous study based on seismological data shows a reverse movement with a minor left-lateral component: Mw=4.2, depth=1500m, strike=169°, dip=61°, rake=42°. We have performed a geodetic and geomechanical analysis of the seismic source event based on ground deformation inferred from DInSAR. We used ascending and descending Sentinel-1 differential interferograms to retrieve the horizontal and vertical components of the co-seismic deformation. Subsequently, we inverted the estimated deformation to obtain the solution of an activated fault using the Okada model. These results shed light on the geomechanical aspects of the event and can help to understand the effects of field operations interacting with pre-existing structural features and active tectonic processes in the Los Humeros caldera.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Mathematical Geodesy and Positionin
Towards a True Author Entry System for CAI
No full textCAI course authors have been faced with the disc tint problem of having to learn an instructional coding language before they can get their courses into the computer. A system has been devised so that an author may easily write his course in English on course planning forms and then a pre-processor will generate the coding which will be input for the machine assembler
A 19.8-mW Eddy-Current Displacement Sensor Interface with Sub-Nanometer Resolution
No full textThis paper presents an eddy-current sensor (ECS) interface intended for sub-nanometer (sub-nm) displacement sensing in hi-tech applications. The interface employs a 126-MHz excitation frequency to mitigate the skin effect, and achieve high resolution and stability. An efficient on-chip sensor offset compensation scheme is introduced which removes sensor-offset proportional to the standoff distance. To assist in the ratiometric suppression of noise and drift of the excitation oscillator, the ECS interface consists of a highly linear amplitude demodulation scheme that employs passive capacitors for voltage-to-current (V2I) conversion. Using a printed circuit board-based pseudo-differential ECS, stability tests were performed which demonstrated a thermal drift of <7.3 nm/°C and long-term drift of only 29.5 nm over a period of 60 h. The interface achieves an effective noise floor of 13.4 pm Hz which corresponds to a displacement resolution of 0.6 nm in a 2-kHz noise bandwidth. The ECS interface is fabricated in TSMC 0.18- μm CMOS technology and dissipates only 19.8 mW from a 1.8-V supply.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Electronic InstrumentationMicroelectronic
A 0.96-mW dB-Linear Variable Gain Amplifier With 0.4-dB Linearity Error Over a 62.4-dB Gain Tuning Range
No full textThis letter presents a low-power dB-linear variable gain amplifier (VGA) with a small linear-in-dB error over a wide gain tuning range. An exponential current ratio is realized in the linear-in-dB control circuit based on the subthreshold I-V characteristic. The VGA is built with subthreshold common-gate transistors as current steering, accurately replicating the exponential current ratio and forming a tunable gain. Implemented in 55-nm CMOS technology, the proposed VGA occupies a compact active area of 0.011 mm2 excluding the buffer. It achieves a linear-in-dB error of 0.4 dB over a gain tuning range of 62.4 dB, corresponding to the state-of-the-art relative error of 0.6%. The proposed design shows constant 80-MHz bandwidth with a power consumption of 0.96 mW. Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Electronic Instrumentatio
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