2,481 research outputs found
Benthic Foraminifers as ecological and biological indicators: a practical approach using experimental cultures and their potential use in coastal monitoring management
No full textTechnical Note: Determination of the metabolically active fraction of benthic foraminifera by means of Fluorescent In Situ Hybridization (FISH)
No full textBenthic foraminifera are an important component of the marine biota, but protocols for investigating their viability and metabolism are still extremely limited. Classical studies on benthic foraminifera have been based on direct counting under light microscopy. Typically, these organisms are stained with Rose Bengal, which binds proteins and other macromolecules, but does not allow discrimination between viable and recently dead organisms. The fluorescent in situ hybridization technique (FISH) represents a new and useful approach to identify living cells possessing an active metabolism. Our work is the first test of the suitability of the FISH technique, based on fluorescent probes targeting the 18S rRNA, to detect live benthic foraminifera. The protocol was applied on <I>Ammonia</I> group and Miliolids, as well as on agglutinated polythalamous (i.e., <I>Leptohalysis scottii</I> and <I>Eggerella scabra</I>) and soft-shelled monothalamous (i.e., <I>Psammophaga</I> sp. and saccamminid morphotypes) taxa. The results from FISH analyses were compared with those obtained, on the same specimens assayed with FISH, from microscopic analysis of the cytoplasm colour, presence of pigments and pseudopodial activity. Our results indicate that FISH targets only metabolically active foraminifera, and allows discerning from low to high cellular activity, validating the hypothesis that the intensity of the fluorescent signal emitted by the probe is dependent upon the physiological status of cells. These findings support the usefulness of this molecular approach as a key tool for obtaining information on the physiology of living foraminifera, both in field and experimental settings
Determination of the metabolically active fraction of benthic foraminifera by means of Fluorescent In Situ Hybridization (FISH)
No full text
Application of the IS-MP-IA model to the German economy and policy implications
No full textExtending the IS-MP-IA model developed by Romer (2000) and applying the GARCH (Engle, 1982, 2001) methodology, the author finds that equilibrium GDP in Germany is positively affected by stock market performance and real exchange rate appreciation, and negatively influenced by the expected inflation rate, the government deficit/GDP ratio, and the U.S. federal funds rate. The relatively low deficit/GDP ratio of 1.83% in 2003 indicates that its fiscal condition was healthy. However, some other EU members may need to exercise fiscal discipline. Because real appreciation has a positive impact on output, a stronger euro may not be a concern for Germany but may be worried by those EU member nations which depend upon exports to stimulate their economies.
Winning redefined, a new brand positioning for MP Motorsport
No full textMP Motorsport is a talent educating race team participating in the classes below the Formula 1. The problem with the lower classes and therefore MP Motorsport as well is that these classes don’t get much attention and exposure. As a company run by people with passion the branding and positioning is more something that naturally emerged from this passion rather than a series of conscious decisions. This makes it fragile. A more conscious strategy and vision on how MP Motorsport needs to be branded and positioned against their competition that is more than “look how cool racing is” is needed to become more attractive for sponsoring. This report describes the process from analysis to finding the right positioning and an advise for a new brand identity and an implementation plan to help becoming more attractive for sponsoring goals. The analysis done with employees showed a unique characteristic that will help the team to position themselves against the competition; the family atmosphere. This atmosphere is what also characterises their contradictory personality. This personality is on one hand leading and ambitious and on the other hand modest and involved. Competition and stakeholder analysis have been performed to find the right combination of being unique to the competition, desirable for the stakeholders (the fans and sponsors) and builds upon the core strengths of the company. Then using the brand key model, a positioning is made with the essence: Be your best self. The belief, “in everyone hides a talent” and values like “everyone is equal” and “together we succeed” combined with the essence and the positioning resulted in a brand story that describes the feeling MP Motorsport wants to communicate. To manifestate the brand, an advise has been set up for a tone of voice and tone of image. Wrapped in a concept called “winning redefined” this advise is part of the whole implementation plan that should lead the way for the team to implement the newly created brand in short and long term actions. In 3 phases MP Motorsport is advised to start with a clear brand introduction to the target groups Gen Z and potential sponsors. The next phase revolves around creating a community to attract both Gen Z talents and subsequently sponsors to interact with the team and each other. Finally the last phase is long term focused and aims for sustainable growth. In this phase the community is established and can expect various opportunities to discover and develop a whole range of talents, sponsors and gen z are connected to each other via MP Motorsport and the team is able to finance the lower classes without the pressure of the money drivers bring along.Strategic Product Desig
Derivations (MP) and Evaluations (OT)
No full textThe main claim of this paper is that the minimalist framework and optimality theory adopt more or less the same architecture of grammar: both assume that a generator defines a set S of potentially well-formed expressions that can be generated on the basis of a given input, and that there is an evaluator that selects the expressions from S that are actually grammatical in a given language L. The paper therefore proposes a model of grammar in which the strengths of the two frameworks are combined: more specifically, it is argued that the computational system of human language CHL from MP creates a set S of potentially well-formed expressions, and that these are subsequently evaluated in an optimality theoretic fashion.The definitive version of this paper is published in Linguistics in Potsdam 25 (2006).Broekhuis, H. (2006). Derivations (MP) and Evaluations (OT)*. In H. Broekhuis & R. Vogel (Eds), Linguistics in Potsdam 25. Optimality Theory and Minimalism: A possible Convergence? Potsdam : Universitätsverlag PotsdamISBN: 9783939469544 (published book)This research is supported by the Netherlands Organisation of Scientific Research (NWO), grant 276-70-00
Stroom- en sedimentmeting Roompot-Veerse Dam (mp.8), 15 en 22 januari 1996
No full textStroom- en sedimentmeting Roompot-Veerse Dam (mp.8) 15 en 22 januari 1996.Deltawerken, Oosterscheld
Analysis of mucosal bacteria in pediatric patients with inflammatory bowel disease
No full textATTI CONGRESSO SIGENP 200
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