87,880 research outputs found

    Genotypic typing of bacterial ecotypes significantly present in natural fermentation of italian dairy products

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    This PhD thesis dealt with the phenotypic and genotypic characterization of LAB strains through a polyphasic taxonomic approach, molecular analyses such as PCR, ITS, ARDRA, the study of housekeeping genes, search and classification of extracromosomal DNA. The integration of the phenotypic, biotechnological and genotypic results allowed the obtainment of fingerprinting specific at the strain-level and the possible discrimination of the strains in function of their ecological niches

    Hydrillae

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    Progettazione di Housing Sociale: un quartiere di edilizia residenziale sociale integrata al borgo storico preesistente, al tessuto rurale, al sistema metropolitano ed infrastrutturale della periferia di Milan

    Borgo Giovanni Antonio da

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    la voce dedicata a Giovanni Antonio da Borgo in "Dizionario dei tipografi degli editori italiani. Il Cinquecento. Vol. 1. A-F" a cura di Marco Menato, Ennio Sandal, Giuseppina Zappella. Milano, Editrice bibliografica, 199

    The nematode Caenorhabditis elegans as an innovative tool for studying foodborne metabolites and emerging pathogens in the food industry

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    In the last few decades, various epidemiological evidence has reinforced the concept that diet plays an important role in human health, showing that the type, composition and amount of food consumed can all be linked to the promotion and/or maintenance of a healthy state of being. Traditional fermented functional foods are increasingly being considered healthy and interest in their nutritional and health impact, as well as demand for them, is continuously increasing. Their health benefits are linked to functional microorganisms. These microorganisms may contribute to food functionality exerting a biogenic effect due to the release of active metabolites and bioactive compounds during food processing. Fermented foods are complex and dynamic systems in which microbial populations interact. The technology for production and conservation of foods can influence the development or inhibition of specific bacterial species which are difficult to detect applying the classical microbiological techniques. The nematode Caenorhabditis elegans represents an innovative tool for studying foodborne metabolites and emerging pathogens in the food industry. This free-living nematode provides several practical and experimental advantages, including a short life-cycle, and easy and economic cultivation in large numbers. Some of the characteristics of the C. elegans immune system are conserved in higher eukaryotic organisms. Together with high-performance functional metagenomic and metabolomic analyses, this nematode represents a good model to investigate the potential adverse effects of food-derived microbial bioactive compounds. The information obtained from these studies will open new avenues for the design of innovative biosensors, suitable for specific and rapid bacterial metabolite detection

    When living things and other 'sensory quality' categories behave in the same fashion: a novel category specificity effect

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    In this study, the performance on semantic tests of five patients with a diagnosis of probable herpes simplex encephalitis was examined. Only one of the patients, MU, showed a marked category-specific deficit for living things, unlike the other patients. Results which closely mirrored those obtained with the category living things were found in each of the five patients for the other categories, edible substances, materials, and liquids, selected for a prior! theoretical reasons. The processing of these additional categories was investigated with tasks involving naming abilities in different modalities, matching to sample, and questionnaires exploring the status of the patients' knowledge about the semantic features of both living things and exemplars of novel 'sensory quality' categories. MU showed in all tasks a comparable impairment for both living things and the other three new categories, in spite of a performance closely equivalent to that of the other four patients with man-made artefacts. This finding supports an explanation of MU's performance in terms of an impairment relating to categories highly dependent on the sensory quality of stimuli. In addition, his difficulty involved all aspects of the processing of the impaired categories

    L'Australia nelle sue relazioni politiche ed economiche con la Cina : luci ed ombre di un rapporto lungo, alterno e complesso

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    The article reconstructs the evolution of the political and economic relations developed between Australia and China from the birth of the Confederation up to the present day, resuming – according to the Australian perspective – the ranks of the relations that have taken place over time between these two countries, which are extremely different in terms of historical political, social, cultural events. The critical examination of a wide range of English-language literature, produced over time by scholars of various disciplines, aims to understand and discuss the various phases of a comparison that –albeit in alternate, discontinuous, contradictory and sometimes conflictual forms – has always been kept alive. The current situation sees both States strongly linked by a delicate and complex connection of political and economic interests, as well as leading players in the “crowded” and dynamic Asia Pacific region. This set of circumstances poses specific questions to Australia about the future of its bilateral relations with China, which – in a short and medium-term perspective – must not be separated from careful planning that also takes due account of the knowledge of the complex development of past experiences

    Genotypic characterization of non starter lactic acid bacteria involved in the ripening of artisanal Bitto PDO cheese

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    Bitto of Valchiavenna, an artisanal Italian cheese produced without the addition of any starter cultures, has been attributed a protected designation origin (PDO) cheese, but the strain composition of the natural microbial population colonizing this traditional dairy product is still unknown. To obtain preliminary information on the non starter lactic acid bacteria involved in its ripening, a total of 136 NSLAB isolates, randomly selected from MRS and M17 agar plates, were collected from three different cheese samples after 120 days of ripening. The new isolates were identified by combining PCR 16S–23S rDNA spacer analyses, partial 16S rRNA gene sequencing, species-specific probes and colony hybridization. Eighty-two isolates, representing 60% of the total strains selected, were homofermentative cocci: 83% of them were enterococci, with Enterococcus durans being the predominant species found. Pediococcus spp. were also isolated, together with strains of Streptococcus thermophilus. Within lactobacilli, 57% of the isolates were identified as Lactobacillus paracasei; Lact. curvatus, Lact. plantarum, Lact. fermentum, were present in a lower amount. The isolates were differentiated at strain-level by Rep-PCR analysis. This is the first effort to microbiological characterization of Valchiavenna’s Bitto; the results suggest the possibility of preserving the wild bacterial population in order to protect the typical organoleptic characteristics of this traditional raw milk cheese and to select new strains for the dairy industry
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