137 research outputs found

    Protocol for assessing bacterial wilt resistance in greenhouse and field conditions. International cooperators’ guide

    No full text
    This protocol is an updated version of “Assessing potato clone field resistance to bacterial wilt” issued in The International Cooperators’ Guide (CIP 2007). The first edition of the protocol presented a standard procedure for field assessment of resistance to bacterial wilt for documenting levels of resistance of advanced potato germplasm. This second edition has included a standardized procedure for greenhouse screening of potato seedlings for bacterial wilt resistance useful for perform genetic studies, parental selection or identification of new sources of resistance in accessions of wild species propagated or maintained as true see

    Status of the draft potato ontology.

    No full text
    The potato ontology is being developed as part of the crop ontology (CO) effort amongst CGIAR centers. The CO serves primarily to harmonize phenotypic and genotypic data for semantic compatibility across diverse, distributed data types; and ontology in general, facilitates the use of terms by both humans and algorithms. Other potential uses include knowledge transfer across species and predictions. The case of potato presents several opportunities to test and evaluate the different uses of ontologies: a) potato has more than 100 tuber-bearing wild relatives, b) the potato genome and that of its sister species, tomato, have recently been sequenced, and c) conserved orthologous set (COS) gene sequences markers have been mapped in the physical and genetic map of potato and tomato. On the other hand potential constraints are: a) potato is outbreeding and has a high level of diversity at genotype and phenotype level; and b) predictivity of transferring knowledge between species has been weak in the past. In this talk we will present main advances and pendings to date around the use of the potato ontology. These include: a) development of a first potato ontology based on the former FAO/IPGRI/CIP morphological descriptor list and trait dictionaries from standard evaluation trial protocols; b) the P4 initiative open to collaborators for phenotyping and gene discovery from the DMDD progeny of the sequenced genome, DM ,and c) a federated web database using biomart for integrating genome scale and field trial data. Current efforts include the addition and standardization of new phenotypes, as well as improving the ontology draft in terms of coverage of data types and cross linking to the general plant as well as to the Solanaceae Phenotype Ontology

    Potatoes

    No full text

    Procedures for the generation of potato tuber families from true seed

    No full text
    This protocol attempts to provide a detailed description of the procedures to produce tuber families in order to orient and facilitate potato research toward more efficient breeding product achievements

    Early generation in vitro assay to identify potato populations and clones tolerant to heat

    No full text
    An efficient in vitro system for early generation selection of heat-tolerant potato breeding materials was tested and validated in field conditions. At the family level, family groups expected to be heat tolerant due to their genetic background were identified as heat tolerant. In the in vitro assay, LTVR × LTVR, an advanced heat-tolerant breeding population developed at CIP, had 100 % of plants with tubers at 18 °C, 73 % at 25 °C and 2 % at 32 °C in the dark. The results from true seed family level in vitro screening at 25 °C and tuber family evaluation under field conditions in Tacna, an arid sub-tropical environment in Southern Peru, were positively correlated (r = 0.57). There was low to moderate correlation between percentage of plants with tubers under 27 °C in vitro temperature treatment and harvest index in the in vivo conditions in Majes–Arequipa, San Ramon, and La Molina that followed increasing temperature ranges between the sites. This indicates that the methodology can predict putative heat tolerant clones with a low level of confidence. Low correlation is possibly due to differential responses of the clones to characteristics of the growing environment, such as soil versus media, which were not represented in the in vitro assay, as well as the fact that in the field, day–night temperatures vary during tuberization and tuber filling, and throughout the season, while in vitro temperature and the dark period were kept constant, and conditions were controlled specifically to assess tuberization (tuber induction) at high night temperatures. The ability of the in vitro seedling screening assay to identify families tolerant to high temperatures in an inexpensive and less time consuming way without need of transplanting experimental material to the field will facilitate evaluation of significant samples of genetic resources and improved populations in breeding programs attempting to improve potato for adaptation to new environments and climate change
    corecore