1,721,161 research outputs found

    Ernia di Richter: a case report. Considerazioni clinico-terapeutiche.

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    [Richter's hernia: a clinical case and the clinico-therapeutic considerations]. Fornaro R, Terrizzi A, Davini MD, Canaletti M, Baldi E, Bonfante P, Sticchi C, Cavaliere D, Ferraris R. The authors report a case of Richter's hernia. They underline main clinical and therapeutic patterns, emphasizing the need of an early diagnosis and surgery. This is a hernia of abdominal wall with partial entrapment of bowel wall (antimesenteric site) through a small ring. The incidence increased in the last years because of diffusion of laparoscopic techniques. Richter's hernia could be asymptomatic for a long time or show vanish sign. Sometimes this hernia can be diagnosed during surgery. The clinical signs are conclamated if hernia is complicated by strangulation. High mortality is justified by performing too late diagnosis and operation

    L’emangiopericitoma renale. Considerazioni anatomo patologiche e clinico-terapeutiche. Descrizione di un caso.

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    [Renal hemangiopericytoma. Anatomo-pathologic and clinico-therapeutic considerations. A case report]. G Chir. 1999 Jan-Feb;20(1-2):20-4. Fornaro R, Terrizzi A, Secco GB, Canaletti M, Baldi E, Bonfante P, Sticchi C, Baccini P, Cittadini G Jr, Fiorini G, Ferraris R. Cattedra di Semeiotica Chirurgica I, Università degli Studi di Genova. The Authors report a case of renal hemangiopericytoma, whose interest is related to the extreme rarity (24 cases reported until today), its insidious growth, the late in diagnosis, its uncertain clinical-biological evolution, not always predictable. Considering chemotherapy and radiotherapy ineffectiveness, an adequate treatment for such a neoplasm requires the surgical therapy, which must be followed by a careful follow-up

    DIFFERENTIAL ABSORPTION AND LOCALIZATION OF 2 SCLEROTINIA- SCLEROTIORUM ENDO-POLYGALACTURONASES IN SOYBEAN HYPOCOTYLS

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    The isolate B-24 of the necrotrophic fungus Sclerotinia sclerotiorum produces two major endo-polygalacturonases (PG-II and PG-IV) during infection of soybean seedlings. By a combination of biochemical, immunofluorescence and morphological techniques it was shown that they were differentially absorbed by soybean hypocotyl tissue, becoming located preferentially at different sites. PG-IV was absorbed much more than PG-II and it was able to permeate the unwounded surface of the hypocotyl and accumulate in the cortical cell layer where it produced an increase of autofluorescence and a marked loss of adhesion of cortical cells. When applied to the cut-end surface of the hypocotyl PG-IV remained localized near the site of the treatment. In contrast PG-II was not absorbed through the epidermis but, when applied to the cut surface of hypocotyl it was transported some distance into the tissue possibly via the vascular bundles, The different behaviour of the two PGs together with their different properties suggest they play a different but complementary role in the pathogenic strategy of S. sclerotiorum

    Early Lotus japonicus root transcriptomic responses to symbiotic and pathogenic fungal exudates

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    The objective of this study is to evaluate Lotus japonicus transcriptomic responses to arbuscular mycorrhizal (AM) germinated spore exudates (GSEs), responsible for activating nuclear Ca2+ spiking in plant root epidermis. A microarray experiment was performed comparing gene expression in Lotus rootlets treated with GSE or water after 24 and 48 h. The transcriptional pattern of selected genes that resulted to be regulated in the array was further evaluated upon different treatments and timings. In particular, Lotus rootlets were treated with: GSE from the pathogenic fungus Colletotrichum trifolii; short chitin oligomers (COs; acknowledged AM fungal signals) and long COs (as activators of pathogenic responses). This experimental set up has revealed that AM GSE generates a strong transcriptomic response in Lotus roots with an extensive defense-related response after 24 h and a subsequent down-regulation after 48 h. A similar subset of defense-related genes resulted to be up-regulated also upon treatment with C. trifolii GSE, although with an opposite trend. Surprisingly, long COs activated both defense-like and symbiosis-related genes. Among the genes regulated in the microarray, promoter-GUS assay showed that LjMATE1 activates in epidermal cells and root hairs
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