162,425 research outputs found
Quantitative analysis of snoRNA association with pre-ribosomes and release of snR30 by Rok1 helicase
No full textIn yeast, three small nucleolar RNAs (snoRNAs) are essential for the processing of pre-ribosomal RNA—U3, U14 and snR30—whereas 72 non-essential snoRNAs direct site-specific modification of pre-rRNA. We applied a quantitative screen for alterations in the pre-ribosome association to all 75 yeast snoRNAs in strains depleted of eight putative helicases implicated in 40S subunit synthesis. For the modification-guide snoRNAs, we found no clear evidence for the involvement of these helicases in the association or dissociation of pre-ribosomes. However, the DEAD box helicase Rok1 was required specifically for the release of snR30. Point mutations in motif I, but not in motif III, of the helicase domain of Rok1 impaired the release of snR30, but this was less marked than in strains depleted of Rok1, and resulted in a dominant-negative growth phenotype. Dissociation of U3 and U14 from pre-ribosomes is also dependent on helicases, suggesting that release of the essential snoRNAs might differ mechanistically from release of the modification-guide snoRNAs. Keywords: ribosome biogenesis; RNA helicase; snoRN
Kim Bohnsack at Windells Snowboard Camp [12]
No full textTwelfth in a series of 14 photos showing Kim Bohnsack snowboarding at Windells snowboard camp near Mount Hood, Orego
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
The box C/D and H/ACA snoRNPs: key players in the modification, processing and the dynamic folding of ribosomal RNA
No full textThe 5S RNP Couples p53 Homeostasis to Ribosome Biogenesis and Nucleolar Stress
No full textSummarySeveral proto-oncogenes and tumor suppressors regulate the production of ribosomes. Ribosome biogenesis is a major consumer of cellular energy, and defects result in p53 activation via repression of mouse double minute 2 (MDM2) homolog by the ribosomal proteins RPL5 and RPL11. Here, we report that RPL5 and RPL11 regulate p53 from the context of a ribosomal subcomplex, the 5S ribonucleoprotein particle (RNP). We provide evidence that the third component of this complex, the 5S rRNA, is critical for p53 regulation. In addition, we show that the 5S RNP is essential for the activation of p53 by p14ARF, a protein that is activated by oncogene overexpression. Our data show that the abundance of the 5S RNP, and therefore p53 levels, is determined by factors regulating 5S complex formation and ribosome integration, including the tumor suppressor PICT1. The 5S RNP therefore emerges as the critical coordinator of signaling pathways that couple cell proliferation with ribosome production
Typen krisenhafter Spannungen zwischen Normen und Orientierungsrahmen. Methodische Reflexionen zu einer Studie über Lehrerkrisen im Berufsalltag.
No full textHinzke J-H. Typen krisenhafter Spannungen zwischen Normen und Orientierungsrahmen. Methodische Reflexionen zu einer Studie über Lehrerkrisen im Berufsalltag. In: Bohnsack R, Nentwig-Gesemann I, Hoffmann N, eds. Typenbildung und Dokumentarische Methode. Forschungspraxis und methodologische Grundlagen. Opladen: Barbara Budrich; 2018: 68-82
Zur Aktualiät und Relevanz der Allgemeinen Didaktik in der Lehrerinnenausbildung
No full textKoch-Priewe B. Zur Aktualiät und Relevanz der Allgemeinen Didaktik in der Lehrerinnenausbildung. In: Bayer M, Bohnsack F, Koch-Priewe B, Wildt J, eds. Lehrerin und Lehrer werden ohne Kompetenz? Professionalisierung durch eine andere Lehrerbildung. Bad Heilbrunn: Klinkhardt; 2000: 148-169
Murder on the mountain: author talk with Peter J. Wosh
No full textAuthor talk by Peter J. Wosh on May 5th, 2022, on his book, "Murder on the Mountain: crime, passion, and punishment in gilded age New Jersey.
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