344 research outputs found
William Blake and the visionary poetry of the law.
No full textPhDThis dissertation examines the meaning of law in Blake's work. I argue that Blake's poetry
intersects with contemporaneous challenges to the traditional model of the ancient constitution,
a debate which I present as a conflict between custom and code. Blake's support for the French
Revolution's overthrow of the customary systems of the ancien regime is countered by his
nervousness about the rights-based discourse advanced by leading radical intellectuals such as
Thomas Paine, a belief that the new systems which they proposed merely re-stated those which
they sought to replace within an even narrower compass.
Law is also a contested ground within radical political discourse of this period; although the
dominant proposals advocated the enshrinement of fundamental rights and the codification of
law, there was also a tendency towards a more enthusiastic radicalism These millenarian
groups, emerging from antinomian heresy, rejected the notion of life being framed within a set
of moral laws. I argue that Blake cannot easily be placed in either group; his work exhibits a
fidelity to the redemptive potential of law, coupled with a real concern that to define freedoms
in legal terms serves to limit rather than to liberate.
Blake's work thus engages with a problem of the period: how to understand the new
discourses of law. The customary account of the ancient English conunon law is predicated on
the idea that it is codified, yet not written down; secular, though grounded in divine principle.
These ambivalences are exploited by Blake in his poetic exploration of the law in the 1790s. In
his nineteenth-century epics, Blake finds increasing help in dissenting religion's reconstruction
of a radicalized Jesus. Through this radical prophetic voice, Blake is able to construct a
redemptive legality founded on a deinstitutio-nalized Christianity, a constitutionalism that is
also recovered from the conventional customary account
The global food chain
No full textJonathon Porritt is Founder Director of Forum for the Future www.forumforthefuture.org.uk; Chairman of the UK Sustainable Development Commission www.sd-commission.org.uk; and author of Capitalism as if the World Matters (Earthscan, 2007)
Senescence-associated DNA methylation is stochastically acquired in subpopulations of mesenchymal stem cells
No full textReplicative senescence has a major impact on function and integrity of cell preparations. This process is reflected by continuous DNA methylation (DNAm) changes at specific CpG dinucleotides in the course of in vitro culture, and such modifications can be used to estimate the state of cellular senescence for quality control of cell preparations. Still, it is unclear how senescence-associated DNAm changes are regulated and whether they occur simultaneously across a cell population. In this study, we analyzed global DNAm profiles of human mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVECs) to demonstrate that senescence-associated DNAm changes are overall similar in these different cell types. Subsequently, an Epigenetic-Senescence-Signature, based on six CpGs, was either analyzed by pyrosequencing or by bar-coded bisulfite amplicon sequencing. There was a good correlation between predicted and real passage numbers in bulk populations of MSCs (R2 = 0.67) and HUVECs (R2 = 0.97). However, when we analyzed the Epigenetic-Senescence-Signature in subclones of MSCs, the predictions revealed high variation and they were not related to the adipogenic or osteogenic differentiation potential of the subclones. Notably, in clonally derived subpopulations, the DNAm levels of neighboring CpGs differed extensively, indicating that these genomic regions are not synchronously modified during senescence. Taken together, senescence-associated DNAm changes occur in a highly reproducible manner, but they are not synchronously co-regulated. They rather appear to be acquired stochastically-potentially evoked by other epigenetic modifications
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Destination-Based Brand Equity: The Impact of Destination Image on the destination stakeholders
No full textDr Jonathon Day
Dr Jonathon Day is an Associate Professor in Purdue’s School of Hospitality and Tourism Management. In addition to over 45 academic articles and chapters, he is the author of “Introduction to Sustainable Tourism and Responsible Travel” and co-author of “The Tourism System 8th Edition” . Dr Day’s research interests focus on sustainable tourism and responsible travel.
Gurpreet Kour
Gurpreet is an assistant professor at the Indian Institute of Management, Shillong. Her research interests include destination barnding and destination governance. Destination image has been an important topic in tourism research. The appeal of a destination’s image can contribute to a destination’s competitiveness. A wide range of factors contribute to the strength of the destination image, and the value extends beyond consumers to destination stakeholders. While a range of issues around destination image have been examined, there remains much to be explored. There is a paucity of research on the value created for these stakeholders by the brand image. There is also a gap in our understanding of the impact of destination image on destination stakeholder behavior. It is anticipated that operators in destinations with an image that emphasizes nature, and the environment will be more likely to undertake environmentally sustainable activities. This study aims to understand how the stakeholder perceives the value of the destination image and the impact of image considerations on stakeholders' actions in a destination
A conceptual review of interprofessional expertise in child safeguarding
No full textIt is increasingly accepted that practitioners across a range of professional fields must work together in order to promote children's welfare and protect them from harm. However, it has also become apparent that interprofessional working is a challenging area of practice that cannot simply be prescribed through protocols and procedures, nor acquired as a set of technical competences. This article develops the concept of interprofessional expertise in order to explain how practitioners become more proficient at working with others to manage complex child welfare issues. Key principles are outlined with reference to relevant theoretical frameworks, including models of skill acquisition. The article concludes by discussing some potential implications for future research and contemporary developments in child safeguarding practice
Allylic C—H activation to access anti-1,3-amino alcohol motifs
No full text1,3-Amino alcohols are common motifs in a variety of biologically active molecules including antivirals, antibiotics, antifungals, and various alkaloids. Due to their prevalence and utility as synthetic intermediates, a variety of methods have been developed to access these motifs in a diastereoselective fashion, which are outlined in detail herein.
This thesis documents a novel approach to access anti-1,3-amino alcohols through an intramolecular palladium (II)/sulfoxide-oxazoline catalyzed C—H functionalization between a terminal olefin and an N-tosyl carbamate, generating anti-1,3-oxazinanones. These motifs can be further elaborated upon, making this method ideal for the late stage diversification of complex molecules and pharmaceuticals. This new method can be carried out in the presence of reactive functionality that is not well tolerated by existing methods.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2020-05-01The student, Jonathon Young, accepted the attached license on 2018-04-25 at 09:29.The student, Jonathon Young, submitted this Thesis for approval on 2018-04-25 at 09:40.This Thesis was approved for publication on 2018-04-25 at 14:08.DSpace SAF Submission Ingestion Package generated from Vireo submission #12470 on 2018-08-31 at 17:30:26Made available in DSpace on 2018-09-04T20:47:30Z (GMT). No. of bitstreams: 2
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Data and script for Van Berkel et al: Can starlings use a reliable cue of future food deprivation to adaptively modify foraging and fat reserves?
No full textSupporting materials for:
Can starlings use a reliable cue of future food deprivation to adaptively modify foraging and fat reserves?
Menno van Berkela, Melissa Batesona, Daniel Nettlea and Jonathon Dunna*
aCentre for Behaviour and Evolution & Institute of Neuroscience, Newcastle University, Newcastle, UK
*Author for correspondence (email: [email protected]; telephone: (+44)7730015855; postal address: Institute of Neuroscience, Henry Wellcome Building, The Medical School, Framlington Place, Newcastle University, Newcastle upon Tyne, UK, NE2 4HH).
R script and 3 .csv files.</p
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A Qualitative Study on the Perceptions of Soft Commitment Devices in Tourism
No full textAilin Fei is a second-year PhD student studying Hospitality and Tourism Management at Purdue University. Ailin earned her bachelors and masters from the University of Houston both in hospitality management. Her research interests are in sustainable tourism, specifically, responsible and sustainable travel behavior. Her recent papers investigate the effects of travel pledges and dives into behavioral economics and sustainable tourism.
Dr. Jonathon Day, an internationally recognized sustainable tourism expert and an Associate Professor in Purdue’s White Lodging – J.W. Marriott, Jr School of Hospitality and Tourism Management, has over 30 years of experience in tourism management. Jonathon is the coauthor of The Tourism System 8th edition, and author of “Introduction to Sustainable Tourism and Responsible Travel”, as well as over 35 peer-reviewed. He was recognized by the International Hospitality Institute as one of the Global 25 most influential educators in hospitality and the Global Top 30 Champions of Environmental Sustainability in Hospitality and Tourism for 2021.The negative impacts of mass tourism is attracting attention and tourism destinations are looking for ways to promote responsible travel behaviors among tourists. Commitment devices are being used to promote such behaviors. There are two types of commitment devices: soft and hard. studies have explored how soft commitment devices are being perceived by tourists. This study will explore the perceptions of three soft commitment devices being implemented in the tourist experience through the tourists’ perception. This study will provide key insights into the positive and/or negative attitudes toward the commitment devices being used to increase sustainable travel behaviors among tourists. Data will be collected through semi-structured interviews. The results of this study will reveal how tourists interpret the use of soft commitment devices and their perceived impact of such tools. This paper provides new insights about the practicalities of soft commitment devices used to promote sustainable travel behavior
Identification of MiR-21-5p as a functional regulator of mesothelin expression using microRNA capture affinity coupled with next generation sequencing
No full textMicroRNAs (miRNAs) are small non-coding RNAs that regulate mRNA expression mainly by silencing target transcripts via binding to miRNA recognition elements (MREs) in the 3′untranslated region (3′UTR). The identification of bona fide targets is challenging for researchers working on the functional aspect of miRNAs. Recently, we developed a method (miR-CATCH) based on biotinylated DNA antisense oligonucleotides that capture the mRNA of interest and facilitates the characterisation of miRNAs::mRNA interactions in a physiological cellular context. Here, the miR-CATCH technique was applied to the mesothelin (MSLN) gene and coupled with next generation sequencing (NGS), to identify miRNAs that regulate MSLN mRNA and that may be responsible for its increased protein levels found in malignant pleural mesothelioma (MPM). Biotinylated MSLN oligos were employed to isolate miRNA::MSLN mRNA complexes from a normal cell line (Met-5A) which expresses low levels of MSLN. MiRNAs targeting the MSLN mRNA were identified by NGS and miR-21-5p and miR-100-5p were selected for further validation analyses. MiR-21-5p was shown to be able to modulate MSLN expression in miRNA mimic experiments in a panel of malignant and non-malignant cell lines. Further miRNA inhibitor experiments and luciferase assays in Mero-14 cells validated miR-21-5p as a true regulator of MSLN. Moreover, in vitro experiments showed that treatment with miR-21-5p mimic reduced proliferation of MPM cell lines. Altogether, this work shows that the miR-CATCH technique, coupled with NGS and in vitro validation, represents a reliable method to identify native miRNA::mRNA interactions. MiR-21-5p is suggested as novel regulator of MSLN with a possible functional role in cellular growth
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