163 research outputs found
Surface Plasmon Resonance Spectroscopy (SPR) interaction studies of the circadian controlled tomato LHCa4*1 (cab 11) protein with its promoter.
No full textHoffrogge R, Mikschofsky H, Piechulla B. Surface Plasmon Resonance Spectroscopy (SPR) interaction studies of the circadian controlled tomato LHCa4*1 (cab 11) protein with its promoter. Chronobiology International. 2003;20(4):543-558
Transcriptional regulation of oscillating steady-state Lhc mRNA levels: characterization of two Lhca promotor fragments in transgenic tobacco plants.
No full textKellmann J-W, Hoffrogge R, Piechulla B. Transcriptional regulation of oscillating steady-state Lhc mRNA levels: characterization of two Lhca promotor fragments in transgenic tobacco plants. Biological Rhythm Research. 1999;30(3):264-271
VOC emission of various Serratia species and isolates and genome analysis of Serratia plymuthica 4Rx13
No full textBacteria emit a wealth of volatile organic compounds. Gas chromatography coupled to mass spectrometry analysis of five Serratia strains revealed ketones, dimethyl di- and trisulfide and 2-phenylethanol commonly released in this genus. The polymethylated bicyclic hydrocarbon sodorifen was uniquely released by the rhizobacterium Serratia plymuthica 4Rx13. Of 10 Serratia strains, only S.plymuthica isolates originating from plants grown on fields near Rostock (Germany) released this new and unusual compound. Since the biosynthetic pathway of sodorifen was unknown, the genome sequence of S.plymuthica 4Rx13 was determined and annotated. Genome comparison of S.plymuthica 4Rx13 with sodorifen non-producing Serratia species highlighted 246 unique candidate open reading frames.DF
A terpene synthase is involved in the synthesis of the volatile organic compound sodorifen of Serratia plymuthica 4Rx13
No full textBacteria release a plethora of volatile organic compounds (VOCs), including compounds with extraordinary structures. Sodorifen (IUPAC name: 1,2,4,5,6,7,8-heptamethyl-3-methylenebicyclo[3.2.1]oct-6-ene) is a recently identified and unusual volatile hydrocarbon that is emitted by the rhizobacterium Serratia plymuthica 4Rx13. Sodorifen comprises a bicyclic ring structure solely consisting of carbon and hydrogen atoms, where every carbon atom of the skeleton is substituted with either a methyl or a methylene group. This unusual feature of sodorifen made a prediction of its biosynthetic origin very difficult and so far its biosynthesis was unknown. To unravel the biosynthetic pathway we performed genome and transcriptome analyses to identify candidate genes. One knockout mutant (SOD_c20750) showed the desired negative sodorifen phenotype. Here it was shown for the first time that this gene is indispensable for the synthesis of sodorifen and strongly supports the hypothesis that sodorifen descends from the terpene metabolism. SOD_c20750 is the first bacterial terpene cyclase isolated from Serratia spp. and Enterobacteriales. Homology modeling revealed a 3D structure, which indicated a functional role of amino acids for intermediate cation stabilization (W325) and putative proton acceptance (Y331). Moreover, the size and hydrophobicity of the active site strongly indicated that indeed the enzyme may catalyze the unusual compound sodorifen
Professorin und Mutter - wie geht das? : Vom alltäglichen Spagat zwischen Familie und akademischer Karriere
No full textRezension zu: Birgit Piechulla (Hrsg.) Professorin und Mutter – wie geht das? : 28 Berichte vom alltäglichen Spagat zwischen Familie und akademischer Karriere. Spektrum Akademischer Verlag, Heidelberg 2011, ISBN 9783827424310, 384 Seiten, gebundene Ausgabe, 24,95 Euro
Eine Struktur- und Funktionsanalyse der floralen Salizylsäure-Carboxyl-Methyltransferase aus Hoya carnosa R. Br.
No full textInnerhalb der floralen Hoya carnosa S-Adenosyl-L-Methionin Salizylsäure-Carboxyl-Methyltransferasen wurde die Bedeutung einzelner Aminosäuren des aktiven Zentrums untersucht. Ein besonderes Augenmerk lag dabei auf der Substratspezifität und der Größe des Substratspektrums. Von primärer Bedeutung für die Unterscheidung der Hauptsubstrate Salizyl- und Benzoesäure waren ein Methionin bzw. Histidin an der Position 156 im Enzym. Die M156H-Mutante zeigte Eigenschaften einer Benzoesäure/Salizylsäure-MT, wobei erst die zusätzliche L216M-Mutation zur bevorzugten Umsetzung von Benzoesäure führte
Changes of the diurnal and circadian (endogenous) mRNA oscillations of the chlorophyll a/b binding protein in tomato leaves during altered day/night (light/dark) regimes
No full text- …
