51 research outputs found
Diversity of antagonistic bacteria isolated from medicinal plant Peganum harmala L.
No full textAbstractThe antimicrobial activity of plant extract of Peganum harmala, a medicinal plant has been studied already. However, knowledge about bacterial diversity associated with different parts of host plant antagonistic to different human pathogenic bacteria is limited. In this study, bacteria were isolated from root, leaf and fruit of plant. Among 188 bacterial isolates isolated from different parts of the plant only 24 were found to be active against different pathogenic bacteria i.e. Escherichia coli, Methicillin-resistant Staphylococcus aureus (MRSA), Enterococcus faecium, Enterococcus faecalis and Pseudomonas aeruginosa. These active bacterial isolates were identified on the basis of 16S rRNA gene analysis. Total population of bacteria isolated from plant was high in root, following leaf and fruit. Antagonistic bacteria were also more abundant in root as compared to leaf and fruit. Two isolates (EA5 and EA18) exhibited antagonistic activity against most of the targeted pathogenic bacteria mentioned above. Some isolates showed strong inhibition for one targeted pathogenic bacterium while weak or no inhibition for others. Most of the antagonistic isolates were active against MRSA, following E. faecium, P. aeruginosa, E. coli and E. faecalis. Taken together, our results show that medicinal plants are good source of antagonistic bacteria having inhibitory effect against clinical bacterial pathogens
Analysis of bacterial communities in sponges and coral inhabiting Red Sea, using barcoded 454 pyrosequencing
No full textComparative metagenomics and characterization of antimicrobial resistance genes in pasteurized and homemade fermented Arabian laban
No full textMetformin attenuate PTZ-induced apoptotic neurodegeneration in human cortical neuronal cells
No full textChitinophaga eiseniae sp. nov., isolated from vermicompost
No full textA Gram-negative, rod-shaped bacterial strain, YC6729T, was isolated from vermicompost collected at Masan, Korea, and its taxonomic position was investigated by a polyphasic taxonomic approach. Strain YC6729Tgrew optimally at 30 °C and at pH 6.5–8.5. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YC6729Tbelongs to the genusChitinophagain the familyChitinophagaceae. It was related most closely toChitinophaga terraeKP01T(96.4 % 16S rRNA gene sequence similarity),Chitinophaga ginsengisegetisGsoil 040T(96.1 %),Chitinophaga arvensicolaIAM 12650T(96.1 %) andChitinophaga pinensisDSM 2588T(93.3 %). Strain YC6729Tcontained MK-7 as the major menaquinone and homospermidine as the major polyamine. The fatty acids of strain YC6729Twere iso-C15 : 0, C16 : 1ω5c, iso-C17 : 03-OH, C16 : 0, anteiso-C18 : 0and/or C18 : 2ω6,9c, iso-C15 : 02-OH and/or C16 : 1ω7c, C14 : 0, iso-C15 : 03-OH, iso-C15 : 1G, C18 : 1ω5c, iso-C15 : 1I and/or C13 : 03-OH, C13 : 02-OH, C16 : 03-OH and unknown fatty acid ECL 13.565. The polar lipid profile contained phosphatidylethanolamine, unknown aminolipids and unknown lipids. The total DNA G+C content of strain YC6729Twas 48.9 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed that strain YC6729Trepresents a novel species of the genusChitinophaga, for which the nameChitinophaga eiseniaesp. nov. is proposed. The type strain is YC6729T( = KACC 13774T = DSM 22224T).</jats:p
Decoding the Impact of Genetic Variants in Gastric Cancer Patients Based on High-Dimensional Copy Number Variation Data Using Next-Generation Knowledge Discovery Methods
No full textObjectives: Despite a reduction in the incidence and mortality rates of gastric cancer (GC), it remains the fifth most frequently diagnosed malignancy globally. A better understanding of the regulatory mechanisms involved in the progression and development of GC is important for developing novel targeted approaches for treatment. We aimed to identify a set of differentially regulated pathways and cellular, molecular, and physiological system development and functions in GC patients infected with H. pylori infection based on copy number variation (CNV) data using next-generation knowledge discovery (NGKD) methods. Methods: In this study, we used our previous CNV data derived from tissue samples from GC patients (n = 33) and normal gastric samples (n = 15) by the comparative genome hybridization (CGH) method using Illumina HumanOmni1-Quad v.1.0 BeadChip (Zenodo Accession No: 1346283). The variant effects analysis of genetic gain or loss of function in GC was conducted using Ingenuity Pathway Analysis (IPA) software. In addition, in silico validation was performed with iPathwayGuide software using high-throughput RNA sequencing (RNAseq) data (GSE83088) from GC patients. Results: We observed 213 unique CNVs in the control group, 420 unique CNVs in the GC group, and 225 common variants. We found that cancer, gastrointestinal diseases, and organismal injury and abnormalities were the three diseases or disorders that were most affected in the GC group. We also identified that the programmed cell death ligand 1 (PD-L1) cancer immunotherapy pathway, T-cell apoptosis, T-cell exhaustion, and Type 1 regulatory T-cell (Tr1 cells) specialization were dysregulated in GC patients. RNAseq data from GC patients showed that the PD-1/PD-L1 pathway was significantly upregulated in GC samples compared with controls. Conclusions: In conclusion, in the present study, we decoded differentially impacted GC-specific diseases and biological functions and pathways based on CNV data using NGKD methods that can be adopted to design personalized therapeutic approaches for patients with GC in a typical clinical milieu
Research Article Decrypting the microRNA signatures in gastric cancer using high-throughput miRNA array coupled with systems biological approaches for precision medicine
No full textCopper and cobalt nanoparticles containing poly(acrylic acid-co-acrylamide) hydrogel composites for rapid reduction of 4-nitrophenol and fast removal of malachite green from aqueous medium
No full textThreonine in broiler diets: an updated review
No full textAbstract
Threonine (Thr) is the third limiting essential amino acid after methionine and lysine in cornsoybean based diets of broilers. Dietary imbalance of Thr, therefore, results in a poor growth performance in broilers. This review summarizes literature data on the known effects of dietary levels of Thr on growth performance, gut morphology, immunity and carcass characteristics in broilers. Due to continuous improvement in genetic potential and management practices for poultry production, dietary Thr requirements are changing. A number of studies have shown that supplementation of Thr in broiler diet at a higher level than the current NRC recommendation (0.74-0.81%), increases body weight gain, feed conversion ratio, and improves gut morphology, carcass quality and immune status, mainly by enhancing the functional capability of digestive system and immune organs (spleen, bursa, and thymus). According to the literature data discussed in this review, the minimal and maximal total dietary Thr levels for healthy birds reared in normal conditions were 0.67 and 0.90% for growth performance, 0.77 and 1.1% for a better gut health, 0.60 and 1.02% for immunity and 0.62 and 0.97% for better carcass characteristics. This background provides impetus to further investigate the exact level of Thr and its effects on growth performance, gut morphology, immunity and carcass characteristics in broilers.</jats:p
Research Article Two marine sponges-associated cultivable bacteria: Diversity and biological activities
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