21 research outputs found
Cell releasing system using light responsive photovoltaic devices
Get PDFTissue and organ regeneration via transplantation of cell bodies in-situ has become an emerging strategy in regenerative medicine. Injected or implanted cells must be available in large enough numbers and suitable delivery methods must be found especially when implantation into the affected tissue is not possible. In addition, better understanding of how to mitigate the inflammatory reactions and how to degrade the cell carriers are needed. Current cell-based therapies are limited to injection or implantation of cell suspensions or implantation of cell sheets. Development of alternative systems to obtain cell sheets or other functional cellular structures are required. We are investigating light treatment as an efficient, safe, and repeatable method to release cells, cellular aggregates, structures or sheets for implantation in regenerative medicine. We hypothesize that cells cultured on the surfaces of a photovoltaic element may be released by applying visible radiation to the element. The light will induce a surface charge on the element causing cells to be released by electrostatic repulsion. Herein, we conducted a study that examines the amount and viability of myoblasts cells released from a photovoltaic element upon light exposure. We investigated (i) the biocompatibility of PV devices, (ii) the utilization of PV devices as cell substrate iii) the release of attached cells from PV surfaces upon light stimulation, and (iv) the progressive release of proliferated cells from PV devices. C2C12 myoblast cells were cultured on sterile silicon based photovoltaic elements with n-type surface under typical cell culture conditions. Upon confluence, the elements were exposed to low power visible lights for 1-2 hours. Cells in the supernatant and those attached were collected and counted. In addition live/dead assays and DAPI staining were performed on the released cells. Cells released by light exposure were re-seeded for further culture. The results showed that approximately 40% of attached cells could be released from the element upon a light exposure. This strategy may be used to release cells or cellular structures for eventual use in regenerative medicine
Green synthesis of copper oxide (CuO) nanoparticles using banana peel extract and their photocatalytic activities
No full textSilicon substrate as a novel cell culture device for myoblast cells
Get PDFBackground: Tissue and organ regeneration via transplantation of cell bodies in-situ has become an interesting strategy in regenerative medicine. Developments of cell carriers to systematically deliver cell bodies in the damage site have fall shorten on effectively meet this purpose due to inappropriate release control. Thus, there is still need of novel substrate to achieve targeted cell delivery with appropriate vehicles. In the present study, silicon based photovoltaic (PV) devices are used as a cell culturing substrate for the expansion of myoblast mouse cell (C2C12 cells) that offers an atmosphere for regular cell growth in vitro. The adherence, viability and proliferation of the cells on the silicon surface were examined by direct cell counting and fluorescence microscopy.
Conclusions: This study explored the biological applications of silicon based PV devices, demonstrating its biocompatibility properties and found useful for culture of cells on porous 2-D surface. The incorporation of silicon substrate has been efficaciously revealed as a potential cell carrier or vehicle in cell growth technology, allowing for their use in cell based gene therapy, tissue engineering, and therapeutic angiogenesis.
Results: It was found that on the silicon surface, cells proliferated over 7 days showing normal morphology, and expressed their biological activities. Cell culture on silicon substrate reveals their attachment and proliferation over the surface of the PV device. After first day of culture, cell viability was 88% and cell survival remained above 86% as compared to the seeding day after the seventh day. Furthermore, the DAPI staining revealed that the initially scattered cells were able to eventually build a cellular monolayer on top of the silicon substrate
Photovoltaic surfaces enable clonal myoblastic cell release using visible light as external stimulation
No full textGrowth-Inhibitory Effect of Chemotherapeutic Drugs Dispensed by Inkjet Bioprinting on Cancer and Non-Cancer Cells
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Targeted Drug Delivery System using Photovoltaic Devices
No full textAdvances in surgical techniques and scientific research, including the development of new cytotoxic drugs and hormonal therapies, have resulted in better treatment options for cancer patients. Despite such encouraging progress, systemic oral or intravenous administration can cause severe cytoxicity, which limits the therapeutic potential of anticancer drugs. Recent discovery using solar cells for targeted drug delivery will pave the way for the development and introduction of innovative targeted therapies with improved efficacy. A photovoltaic cell holds opposite charges on its surfaces, serve as a new drug delivery system to carry cancer chemotherapeutic drugs or substances and release them when the charge intensity or polarity changes upon external photo stimulation or laser source. In this new strategy using photovoltaic device, a hypothesis is proposed to serve as a new drug delivery method. Positively charged Poly-L-Lysine and negatively charged Bovine Serum Albumin are attached the negative side and positive side of a solar cell respectively. Experimental data reveals that the PV cells significantly can release the charged molecules upon external photo stimulation, which suggests the PV has potential to be used as a new drug delivery system to carry cancer chemotherapeutic drugs
An overview of statistical methods for handling non-adherence to intervention protocol in randomised control trials (RCTs): A methodological review
Get PDFThis is the author accepted manuscript. The final version is available from Elsevier via the DOI in this recordOBJECTIVE: To undertake a methodological review of statistical methods used in randomised controlled trials (RCTs) for handling intervention non-adherence. STUDY DESIGN: Bibliographic databases were searched using predefined search terms. RESULTS: A substantive number of identified studies (56%) were excluded as they only used naive per-protocol (PP) analysis for handling non-adherence. Our review included 58 articles published between 1991 to 2015. A total of 88 methodological applications were made by these studies. The two most used methods were Complier Average Causal Effect (CACE) (56%) and Instrumental Variable (IV) (23%) predominantly with the use of maximum Likelihood (ML) estimators. These alternative applications typically produced treatment effects greater than the intention-to-treat (ITT) effect but as their standard errors were larger there was no statistical difference between the methods. CONCLUSION: A substantive proportion of RCTs rely on naive PP for handling non-adherence. Recent years have seen an increasing number of applications of more appropriate statistical methods, in particular CACE and IV methods. However, these later methods rely on strong underlying assumptions that may be vulnerable to violation. More empirical studies are needed that directly compare the usability and performance of different statistical methods for non-adherence in RCTs
Hypothermia for moderate or severe neonatal encephalopathy in low-income and middle-income countries (HELIX): a randomised controlled trial in India, Sri Lanka, and Bangladesh
No full textCopyright (c) 2021 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license
