1,888 research outputs found
Mass spectrometry–based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count.
No full textThe spliceosome undergoes major changes in protein and RNA composition during pre-mRNA splicing. Knowing the proteins—and their respective quantities—at each spliceosomal assembly stage is critical for understanding the molecular mechanisms and regulation of splicing. Here, we applied three independent mass spectrometry (MS)–based approaches for quantification of these proteins: (1) metabolic labeling by SILAC, (2) chemical labeling by iTRAQ, and (3) label-free spectral count for quantification of the protein composition of the human spliceosomal precatalytic B and catalytic C complexes. In total we were able to quantify 157 proteins by at least two of the three approaches. Our quantification shows that only a very small subset of spliceosomal proteins (the U5 and U2 Sm proteins, a subset of U5 snRNP-specific proteins, and the U2 snRNP-specific proteins U2A′ and U2B′′) remains unaltered upon transition from the B to the C complex. The MS-based quantification approaches classify the majority of proteins as dynamically associated specifically with the B or the C complex. In terms of experimental procedure and the methodical aspect of this work, we show that metabolically labeled spliceosomes are functionally active in terms of their assembly and splicing kinetics and can be utilized for quantitative studies. Moreover, we obtain consistent quantification results from all three methods, including the relatively straightforward and inexpensive label-free spectral count technique
SERGEY YURIEVICH PREOBRAZHENSKY, SCHOLAR AND AUTHOR OF VERSES
No full textThe article is written in memoria of Sergey Yurievich Preobrahzhensky, linguist and poet. It tackles upon the scientific interests of the scholar, deals with his principal concepts in prosody and other spheres of poetics. Besides, It also contains a brief characteristics and examples of his own poetic work
3D cryo-EM structure of an active step I spliceosome and localization of its catalytic core.
No full textThe spliceosome excises introns from pre-mRNA in a two-step splicing reaction. So far, the three-dimensional (3D) structure of a spliceosome with preserved catalytic activity has remained elusive. Here, we determined the 3D structure of the human, catalytically active step I spliceosome (C complex) by cryo-electron microscopy (cryo-EM) in vitrified ice. Via immunolabeling we mapped the position of the 5′ exon. The C complex contains an unusually salt-stable ribonucleoprotein (RNP) core that harbors its catalytic center. We determined the 3D structure of this RNP core and also that of a post-step II particle, the 35S U5 snRNP, which contains most of the C complex core proteins. As C complex domains could be recognized in these structures, their position in the C complex could be determined, thereby allowing the region harboring the spliceosome's catalytic core to be localized
Sergey Witte and his Foreign Investment Policy in the studies by English-speaking scholars
No full text. The article discusses the development of the interest of English-speaking historians in the foreign investment policy of Sergey Witte. The paper also examines the role of the Secret Memorandum of Sergey Wittein the understanding of the foreign investment in the Russian economy. The author shows that Russian and English-speaking historians, despite the political upheavals of the 20th century, were engaged in a scholarly conversation in their discussion of the subject
An Overview of Economics
No full textI assume that your objective of ’Economics 101’ is to try to understand how the economy works instead of going through an economic curriculum. If so you are guaranteed to have fun. I personally undertook a similar journey ten years back. I would say that it is better to understand the principles of economics and then try to understand the economy/economic situation of any given country. My recommendations (for BSc students and everyone who is interested in economics101) are based on my own readings: Sloman’s ”Essentials of economics;” Gwartney et al. ”Economics: Private Public Choice;” Jesus Huerta de Soto’s ”The Austrian School: Market Order and Entrepreneurial Creativity;” Rodrik’s ”Economics Rules;” Foley’s ”Adam’s fallacy: a guide to economic theology;” De Soto’s ”The mystery of capital: Why capitalism triumphs in the West and fails everywhere else;” Harford’s ”The Undercover Economist;” Levitt and Dubner’s ”Freakonomics.”This Paper should not be reported as representing the views of Central Bank of Armenia. The views in this paper are those of the author and should not be interpreted as those of Central Bank of Armenia
An Overview of Economics
No full textI assume that your objective of ’Economics 101’ is to try to understand how the economy works instead of going through an economic curriculum. If so you are guaranteed to have fun. I personally undertook a similar journey ten years back. I would say that it is better to understand the principles of economics and then try to understand the economy/economic situation of any given country. My recommendations (for BSc students and everyone who is interested in economics101) are based on my own readings: Sloman’s ”Essentials of economics;” Gwartney et al. ”Economics: Private Public Choice;” Jesus Huerta de Soto’s ”The Austrian School: Market Order and Entrepreneurial Creativity;” Rodrik’s ”Economics Rules;” Foley’s ”Adam’s fallacy: a guide to economic theology;” De Soto’s ”The mystery of capital: Why capitalism triumphs in the West and fails everywhere else;” Harford’s ”The Undercover Economist;” Levitt and Dubner’s ”Freakonomics.”This Paper should not be reported as representing the views of Central Bank of Armenia. The views in this paper are those of the author and should not be interpreted as those of Central Bank of Armenia
Plato’s Gorgius: an attempt to an axiological commentary
No full textIn his new paper Sergey S. Avanesov (Tomsk State University) analyses the concepts of ‘intermediate’ being, ‘preference’ and the hierarchy of values developed in Plato’s dialogue against the background of Classical moral philosophy. This study of Ancient axiology continues a previous article by the same author, also published in this journal (vol. I, issue 2)
Modern creator in Sergey Samsonov\u27s novel The Kamlayev Anomaly
No full textВ статье рассматривается роман "Аномалия Камлаева" Сергея Самсонова. В центре внимания проблема творца и его творения. Автор максимально укрупняет понятие творчества, выводя его из сферы музыки в сферу самой жизни. Самсонов анализирует состояние человека 21 века и причины его духовного кризиса. Творчество Самсонова рассматривается в контексте нового реализма, сложившегося в русской литературе в конце 20 века. Творческий метод Самсонова также соотносится с дискуссионной концепцией метамодернизма.The article is devoted to the novel of Sergey Samsonov – The Kamlayev Anomaly. The focus is on the issue of a creative man and his creations. The author maximizes the notion of creative work, going beyond the limits of the sphere of music and bringing it to the sphere of life itself. Samsonov is trying to analyze the state of being of the 21st century human and the cause of human spiritual crisis. Samsonov\u27s writing is considered in the context of the new realism. Author\u27s creative method is also compared with the debatable concept of metamodermism
The Latest Version of Circumstances and Cause of Death of Sergey Yesenin. From Order to Exhumation
No full textThe article presents the latest version of circumstances and cause of death of Sergey Yesenin. It should be seen as an edition as well as addition to the recently published monograph Murder of Sergey Yesenin. Criminalistic, Historical and Literary Study (Szczytno 2009), where the primary version of the poet’s murder was depicted. The following revised version reveals some new figures which were directly involved in the tragic death of Yesenin, i.e. Iosif Stalin as the initiator or Georgy Ustinov and Vasily Nazarov as the executors of that perfidious deed. The author projects a plan of the plot, a calendar of the last days and hours of Yesenin’s life, brings out unknown facts and hypotheses which prove a planned homicide committed by members of Soviet secret police (Joint State Political Directorate) and, therefore, disqualify the official version of suicide
Poland and Poles in the dilogy of Sergey Lukyanenko „Rough Draft”, „Final Draft”
No full textThe research material in this work is the dilogy of Sergey Lukyanenko Rough Draft (Черновик, 2005) and Final Draft (Чистовик, 2007). In the first part (Rough Draft) the author focuses on the image of modern Russia, while in the second part (Final Draft) he expands his observations into Poland and its people. The image presented by the author notably duplicates ethnic and cultural stereotypes about Poland and Poles, fixed in the common consciousness of Russians. As a result, despite the objectivity declared by the author, it is difficult to consider the discussed novels
a breakthrough in the context of the process of overcoming mutual prejudices between the neighboring nations on the basis of the literature
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