91 research outputs found
Het Eems estuarium
No full textBundel met twee versies van het plan van Ferguson voor verbetering van het Eemsestuarium, het verslag over berekeningen inzake de waterbeweging in het Eemsestuarium, in verband met zijn normalisatie van Dronkers, het overzicht van de uitkomsten van getijberekeningen betreffende het Eemsestuarium bij verruimd Doekegat en resp. bij geheel of gedeeltelijk afgesloten Dollart, de regeringsnota: Aanvullend memorandum van de Nederlandsche Regeering met betrekking tot de vaststelling van de toekomstige Nederlandsch-Duitsche grens en aanverwante problemen (25-2-1947) en enkele opmerkingen over het Eems/Dollart probleem door de Directeur-Generaal van Rijkswaterstaat, Dibbits
Detection of a frameshift deletion in the SPTBN4 gene leads to prevention of severe myopathy and postnatal mortality in pigs
No full textRepository for the study: Martijn F.L. Derks, Barbara Harlizius, Marcos S. Lopes, Sylvia W.M. Greijdanus-van der Putten, Bert Dibbits, Kimberley Laport, Hendrik-Jan Megens, Martien A.M. Groenen. Detection of a frameshift deletion in the SPTBN4 gene leads to prevention of severe myopathy and postnatal mortality in pigs. 2019. Published in Frontiers in Genetics, doi: 10.3389/fgene.2019.0122
Het deltaplan en zijn verschillende facetten
No full textBundel met artikelen over de waterloopkundige en waterbouwkundige achtergronden van het Deltaplan. Het artikel van Van Veen gaat in op de wordingsgeschiedenis van het Deltaplan, de plannen van voor 1953 en de analoge wiskundige modellen (deltar). De Vries gaat in op de planvorming en de besluitvorming van de Deltacommissie t.a.v. de afsluitingswerken in Haringvliet, Brouwershavense Gat en Oosterschelde. De stormvloedkering in de Hollandse IJssel wordt besproken. Tenslotte gaat Dibbits in op de afluitingsmethoden met name met caissons
Detection of a frameshift deletion in the SPTBN4 gene leads to prevention of severe myopathy and postnatal mortality in pigs
No full textRepository for the study:
Martijn F.L. Derks, Barbara Harlizius, Marcos S. Lopes, Sylvia W.M. Greijdanus-van der Putten, Bert Dibbits, Kimberley Laport, Hendrik-Jan Megens, Martien A.M. Groenen. Detection of a frameshift deletion in the SPTBN4 gene leads to prevention of severe myopathy and postnatal mortality in pigs. 2019.
Published in Frontiers in Genetics, doi: 10.3389/fgene.2019.0122
Mapping carcass and meat quality QTL on <it>Sus Scrofa </it>chromosome 2 in commercial finishing pigs
No full textAbstract Quantitative trait loci (QTL) affecting carcass and meat quality located on SSC2 were identified using variance component methods. A large number of traits involved in meat and carcass quality was detected in a commercial crossbred population: 1855 pigs sired by 17 boars from a synthetic line, which where homozygous (A/A) for IGF2. Using combined linkage and linkage disequilibrium mapping (LDLA), several QTL significantly affecting loin muscle mass, ham weight and ham muscles (outer ham and knuckle ham) and meat quality traits, such as Minolta-L* and -b*, ultimate pH and Japanese colour score were detected. These results agreed well with previous QTL-studies involving SSC2. Since our study is carried out on crossbreds, different QTL may be segregating in the parental lines. To address this question, we compared models with a single QTL-variance component with models allowing for separate sire and dam QTL-variance components. The same QTL were identified using a single QTL variance component model compared to a model allowing for separate variances with minor differences with respect to QTL location. However, the variance component method made it possible to detect QTL segregating in the paternal line (e.g. HAMB), the maternal lines (e.g. Ham) or in both (e.g. pHu). Combining association and linkage information among haplotypes improved slightly the significance of the QTL compared to an analysis using linkage information only.</p
Bioinformatics' approaches to detect genetic variation in whole genome sequencing data
No full textCurrent genetic marker repositories are not sufficient or even are completely lacking for most farm animals. However, genetic markers are essential for the development of a research tool facilitating discovery of genetic factors that contribute to resistance to disease and the overall welfare and performance in farm animals. By large scale identification of Single Nucleotide Polymorphisms (SNPs) and Structural Variants (SVs) we aimed to contribute to the development of a repository of genetic variants for farm animals. For this purpose bioinformatics data pipelines were designed and validated to address the challenge of the cost effective identification of genetic markers in DNA sequencing data even in absence of a fully sequenced reference genome. To find SNPs in pig, we analysed publicly available whole genome shotgun sequencing datasets by sequence alignment and clustering. Sequence clusters were assigned to genomic locations using publicly available BAC sequencing and BAC mapping data. Within the sequence clusters thousands of SNPs were detected of which the genomic location is roughly known. For turkey and duck, species that both were lacking a sufficient sequence data repository for variant discovery, we applied next-generation sequencing (NGS) on a reduced genome representation of a pooled DNA sample. For turkey a genome reference was reconstructed from our sequencing data and available public sequencing data whereas in duck the reference genome constructed by a (NGS) project was used. SNPs obtained by our cost-effective SNP detection procedure still turned out to cover, at intervals, the whole turkey and duck genomes and are of sufficient quality to be used in genotyping studies. Allele frequencies, obtained by genotyping animal panels with a subset our SNPs, correlated well with those observed during SNP detection. The availability of two external duck SNP datasets allowed for the construction of a subset of SNPs which we had in common with these sets. Genotyping turned out that this subset was of outstanding quality and can be used for benchmarking other SNPs that we identified within duck. Ongoing developments in (NGS) allowed for paired end sequencing which is an extension on sequencing analysis that provides information about which pair of reads are coming from the outer ends of one sequenced DNA fragment. We applied this technique on a reduced genome representation of four chicken breeds to detect SVs. Paired end reads were mapped to the chicken reference genome and SVs were identified as abnormally aligned read pairs that have orientation or span sizes discordant from the reference genome. SV detection parameters, to distinguish true structural variants from false positives, were designed and optimized by validation of a small representative sample of SVs using PCR and traditional capillary sequencing. To conclude: we developed SNP repositories which fulfils a requirement for SNPs to perform linkage analysis, comparative genomics QTL studies and ultimately GWA studies in a range of farm animals. We also set the first step in developing a repository for SVs in chicken, a relatively new genetic marker in animal sciences. <br/
\u27In Turkije gaat het tegenwoordig net zo.\u27 De culturele repertoires van een Turks gezin in een multi-etnische wijk
No full textCultural Repertoires of a Turkish Family in a Multiethnic Neighborhood.
This article deals with the relation between processes of ethnicization and integration, based on an analysis of the cultural repertoires of one Turkish family visited by the author weekly for about 18 months. Living in a multiethnic neighborhood, the different members of the family show a strong Turkish ethnic consciousness with regard to language use, social network, TV viewing, food preferences, the celebration of calendar feasts, and religious orientation. However, their ethnic identity cannot be regarded as static; it is constantly redefined by discussions with family and friends. In this process, Turkish TV plays an important role in presenting images of modern Turkey. In daily life, sharp distinctions are made between different situational contexts; conventions in ways of behavior differ according to the situation and are adapted, which allows for a selective appropriation of "new" rituals, eg, celebration of birthdays. Differences between the cultural repertoires of Turkish people in Germany and those in Utrecht are discussed, identifying the need for more systematic research into the role of the local context in processes of ethnicization and integration. The impact of the presence of a large Moroccan minority in the Netherlands is assessed. It is suggested that the attitude of Dutch people toward various aspects of Turkish culture should be taken into account when analyzing processes of ethnicization and integration. Daily life in a multiethnic neighborhood strengthens ethnic consciousness and the idea of being "different," a process that may be accompanied by a quest for new symbols of distinction if old ones have become too common. 28 References. Adapted from the source document
Application of massive parallel sequencing to whole genome SNP discovery in the porcine genome
No full textAbstract Background Although the Illumina 1 G Genome Analyzer generates billions of base pairs of sequence data, challenges arise in sequence selection due to the varying sequence quality. Therefore, in the framework of the International Porcine SNP Chip Consortium, this pilot study aimed to evaluate the impact of the quality level of the sequenced bases on mapping quality and identification of true SNPs on a large scale. Results DNA pooled from five animals from a commercial boar line was digested with DraI; 150–250-bp fragments were isolated and end-sequenced using the Illumina 1 G Genome Analyzer, yielding 70,348,064 sequences 36-bp long. Rules were developed to select sequences, which were then aligned to unique positions in a reference genome. Sequences were selected based on quality, and three thresholds of sequence quality (SQ) were compared. The highest threshold of SQ allowed identification of a larger number of SNPs (17,489), distributed widely across the pig genome. In total, 3,142 SNPs were validated with a success rate of 96%. The correlation between estimated minor allele frequency (MAF) and genotyped MAF was moderate, and SNPs were highly polymorphic in other pig breeds. Lowering the SQ threshold and maintaining the same criteria for SNP identification resulted in the discovery of fewer SNPs (16,768), of which 259 were not identified using higher SQ levels. Validation of SNPs found exclusively in the lower SQ threshold had a success rate of 94% and a low correlation between estimated MAF and genotyped MAF. Base change analysis suggested that the rate of transitions in the pig genome is likely to be similar to that observed in humans. Chromosome X showed reduced nucleotide diversity relative to autosomes, as observed for other species. Conclusion Large numbers of SNPs can be identified reliably by creating strict rules for sequence selection, which simultaneously decreases sequence ambiguity. Selection of sequences using a higher SQ threshold leads to more reliable identification of SNPs. Lower SQ thresholds can be used to guarantee sufficient sequence coverage, resulting in high success rate but less reliable MAF estimation. Nucleotide diversity varies between porcine chromosomes, with the X chromosome showing less variation as observed in other species.</p
50 jaar vereniging van ingenieurs van de Rijkswaterstaat en de Zuiderzeewerken 1918-1969
No full textViertal toespraken ter gelegenehid van het 50-jarig bestaan van de Verenig ing van Ingenieurs van de Rijkswaterstaat en de Zuiderzeewerken, gehouden op donderdag 26 september 1968 in de schouwburg te Middelburg
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