574 research outputs found
Cell-type–specific transcriptome and histone modification dynamics during cellular reprogramming in the Arabidopsis stomatal lineage
No full textPlant cells maintain remarkable developmental plasticity, allowing them to clonally reproduce and to repair tissues following wounding; yet plant cells normally stably maintain consistent identities. Although this capacity was recognized long ago, our mechanistic understanding of the establishment, maintenance, and erasure of cellular identities in plants remains limited. Here, we develop a cell-type?specific reprogramming system that can be probed at the genome-wide scale for alterations in gene expression and histone modifications. We show that relationships among H3K27me3, H3K4me3, and gene expression in single cell types mirror trends from complex tissue, and that H3K27me3 dynamics regulate guard cell identity. Further, upon initiation of reprogramming, guard cells induce H3K27me3-mediated repression of a regulator of wound-induced callus formation, suggesting that cells in intact tissues may have mechanisms to sense and resist inappropriate dedifferentiation. The matched ChIP-sequencing (seq) and RNA-seq datasets created for this analysis also serve as a resource enabling inquiries into the dynamic and global-scale distribution of histone modifications in single cell types in plants.Fil: Lee, Laura R.. University of Stanford; Estados UnidosFil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Bergmann, Dominique C.. University of Stanford; Estados Unido
Timelapse images for Gong, Dale, Fung and Amador et al., 2023
No full textTiled, Z-projected and compressed (from 32-bit to 8-bit) images of timelapse data used in:
A cell size threshold triggers commitment to stomatal fate in Arabidopsis
Yan Gong1†‡, Renee Dale2‡, Hannah F. Fung1‡, Gabriel O. Amador3‡, Margot E. Smit1,4 and Dominique C. Bergmann1,4,*
Science Advances, DOI ______________
A timelapse of WT cotyledons
3 movies
pML1::mCherry-RCI2A
pBRXL2::BRXL2-YFP
2018-12-03
A timelapse of ctr1 cotyledons
3 movies
pML1::mCherry-RCI2A
pBRXL2::BRXL2-YFP
2019-12-19
A timelapse of pSPCH::gSPCH-YFP
1 movie + 1 still
pML1::mCherry-RCI2A
pSPCH::gSPCH-YFP
2021-05-25
A timelapse of H2B in the diploid
1 movie + 1 still
pML1::mCitrine-RCI2A
pML1::H2B-TFP
2022-08-01
A timelapse of H2B in the tetraploid
1 movie + 1 still
pML1::mCitrine-RCI2A
pML1::H2B-TFP
2021-12-10
A timelapse of H2B in the crwn1 mutant
1 movie + 1 still
pML1::mCitrine-RCI2A
pML1::H2B-TFP
2022-07-09</p
Factors that affect short-term commercial bank lending to developing countries
No full textDeveloping countries rely on short-term trade credits for imports of several essential consumer goods, including medicines and basic food supplies. The credits also facilitate export-related transactions. The mechanisms commercial banks use to provide trade credits to developing countries are complex and costly. Even a temporary break in the flow of short-term credit can seriously hurt a country's business. But since short-term trade credits can be structured so that they involve a few risks to a bank and at the same time are very costly to the debtor, they are generally the last forms of credit to be cut and the first to be reestablished in debt-distressed developing countries. To gauge the likelihood of continued short-term trade related financial flows to developing countries, the authors examined the factors that affect short-term commercial bank loans. They studied relevant data over time for seven countries for which data were available: Argentina, Brazil, Egypt,India, Kenya, Mexico, and Turkey. They found that : a) countries with greater growth prospects get more short-term credit; b) short-term credits are usually meant to finance countries with significant trade deficits; c) higher levels of external indebtedness are generally coupled with higher levels of short-term indebtedness to commercial banks; and d) country-specific factors affect the volume of short-term lending to a country.Financial Intermediation,Banks&Banking Reform,Economic Theory&Research,Strategic Debt Management,Financial Crisis Management&Restructuring
Les écrits sur l'art de Dominique Fourcade (la naissance d'une poétique)
No full textLes écrits sur l art forment une partie très importante de l œuvre de Dominique Fourcade : une cinquantaine de textes publiés en préface dans des catalogues d exposition ou dans des revues, principalement entre 1971 et 1990. Beaucoup d entre eux sont consacrés à l œuvre de Matisse, dont Dominique Fourcade est un spécialiste éminent. D autres examinent la création française contemporaine, en particulier les productions des plasticiens proches du galeriste parisien Jean Fournier. Certains étudient, enfin, le travail des artistes anglo-saxons, essentiellement celui des sculpteurs, mais aussi celui des peintres du mouvement expressionniste abstrait. La plupart de ces textes sont majoritairement méconnus même par des spécialistes de la poésie contemporaine. Or, ils sont le lieu où se forge véritablement la vision poétique de l auteur, car c est sur les arts plastiques que Dominique Fourcade prend appui pour penser le monde et trouver la modernité. Dans son travail critique le poète réunit deux approches habituellement opposées : d un côté, la théorie formaliste qui met en valeur les matériaux et les procédés plastiques, de l autre, la recherche d un profond message spirituel des œuvres. Ainsi, les aspects formels (le rythme, la structure, la surface, la lumière, la couleur et d autres encore) comprennent de multiples implications poétiques, dans leurs valeurs éthique, politique ou gnoséologique. Dans ses écrits sur l art, Dominique Fourcade met en place une poétique complexe dans laquelle la sagesse est inséparable de la beauté et l esthétique de l éthique. C est cette même poétique qui réside au cœur de ses poèmes des années 1980-2010.Art publications are an important part of Dominique Fourcade s writing: representing about 50 texts published as prefaces for exhibition catalogues or in the magazines, mainly between 1971 and 1990. A large number of these publications are dedicated to Matisse s work, Dominique Fourcade being an eminent specialist of his work. Others examine contemporary French creation, and especially plastic artists close to Parisian art dealer Jean Fournier. Finally, some of them study Anglo-Saxon artists work, especially that of sculptors, but also of painters belonging to the abstract expressionist movement. The majority of these texts remain unknown, even by the specialists of contemporary poetry. Though, they are the place where the author s poetic vision has been build up, as Dominique Fourcade bases his ideas on plastic art in order to think about world and find modernity. In his critical work, the poet gets together two usually opposite approaches: on the one hand, a formalist theory highlighting materials and plastic methods, on the other, a search for the profound spiritual message of works. Thus, formal aspects (such as rhythm, structure, surface, light, color and others) include multiple poetic implications in their ethical, political or epistemological values. In his art essays, Dominique Fourcade puts in place an intricate poesy where wisdom is inseparable from beauty and aesthetics from ethics. The same poetic language lies in the heart of his poems dating from 1980-2010.NANTERRE-PARIS10-Bib. élec. (920509901) / SudocSudocFranceF
Mechanisms of stomatal development: an evolutionary view
No full textAbstract Plant development has a significant postembryonic phase that is guided heavily by interactions between the plant and the outside environment. This interplay is particularly evident in the development, pattern and function of stomata, epidermal pores on the aerial surfaces of land plants. Stomata have been found in fossils dating from more than 400 million years ago. Strikingly, the morphology of the individual stomatal complex is largely unchanged, but the sizes, numbers and arrangements of stomata and their surrounding cells have diversified tremendously. In many plants, stomata arise from specialized and transient stem-cell like compartments on the leaf. Studies in the flowering plant Arabidopsis thaliana have established a basic molecular framework for the acquisition of cell fate and generation of cell polarity in these compartments, as well as describing some of the key signals and receptors required to produce stomata in organized patterns and in environmentally optimized numbers. Here we present parallel analyses of stomatal developmental pathways at morphological and molecular levels and describe the innovations made by particular clades of plants.</p
Voucher privatization with investment funds : an institutional analysis
No full textCommon wisdom among post-socialist reformers has beento use voucher investment funds to provide the corporate governance needed to restructure newly privatized enterprises after mass privatization efforts. The idea has been that mass privatization would spread the ownership too wide and make corporate governance difficult. The author examines the likely institutional behavior of voucher funds and the possible effects of their development on a transition economy. Since most policy advice has been in favor of voucher privatization with investment funds, the author can be seen as playing the devil's advocate, but his argument is institutional, not statistical. Policymaking requires insight and foresight into how institutions will tend to function. He concludes that voucher funds will introduce a bias in the economy away from the real industrial sector toward an ersatz"financial sector"that will have little if any positive financial role but will be well-protected by friendly regulators. One long-term consequence of voucher privatization with investment funds, according to this view, is a de facto"industrial policy"of real sector decapitalization in favor of short-term rent-seeking by fund managers through board sinecures and lucrative side deals with portfolio companies and through financial market manipulation and paper entrepreneurship in the"financial sector."Without strong corporate governance from the funds and without stable ownership of their own, many enterprise managers will exploit the post-socialist version of the"separation of ownership and control"to grab what they can in the form of salaries, bonuses, perquisites, and side deals. The most likely results of the strategy of voucher privatization with investment funds may be a two-sided grab fest by fund managers and enterprise managers -- together with the accompanying drift, stagnation, and decapitalization of the privatized industrial sector.Economic Adjustment and Lending,Payment Systems&Infrastructure,International Terrorism&Counterterrorism,Economic Theory&Research,Banks&Banking Reform,International Terrorism&Counterterrorism,Banks&Banking Reform,Economic Adjustment and Lending,Environmental Economics&Policies,Economic Theory&Research
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