1,720,987 research outputs found
Phytochemical Elucidation and Antioxidant Activity of Seeds from Three Prickly Pear (Opuntia ficus-indica L.) Cultivars from Algeria
No full textIn this study, the characterization of fatty acids and secondary metabolites in seeds of three cultivars of Opuntia ficus-indica L. (O. ficus-indica, yellow, orange, and green) harvested from the Souk Ahras area in Northeast Algeria was performed. The antioxidant activity of seed extracts was also assessed by using two methods, namely FRAP and DPPH tests. Results show that total phenolic content (TPC) ranged from 63.02 to 81.80 mg gallic acid equivalents (GAE)/100 g of seeds. The yellow cultivar had the highest TPC, followed by the orange and green cultivars. Concerning flavonoids and tannins, the orange cultivar showed higher concentrations, corresponding to 2.97 mg quercetin equivalent (QE)/100 g and 5.60 mg catechin equivalent (CE)/100 g, respectively. Analysis of the seed extracts by HPLC revealed the presence of phenolic compounds, including gallic acid and chlorogenic acid, while the GC analysis of oil showed that prickly pear oil is a major source of essential fatty acids (C18:2). The antioxidant activities of extracts from the three cultivars were comparable. The EC50 for the reduction of ferric iron was almost 0.05 g/mL for all extracts. Regarding the scavenging of DPPH, green and yellow cultivars showed the highest capacity (EC50 = 0.26 g/mL). Linear correlations between the content of some antioxidants (flavonoids, tannins) and different activities were observed, indicating their participation in the latter. Above all, a significant inverse correlation between the total flavonoid content and the EC50 calculated for the reducing activity of seed extracts was observed (r = -0.657; p <= 0.05). Overall, the results indicate that the seeds of O. ficus-indica growing in Algeria can be exploited as valuable sources of table oil, cooking oil, and antioxidants
A new chemotype with high tricyclene content from the essential oil of Salvia aegyptiaca L. growing in Algerian Pre-Sahara
No full textThe essential oil obtained by hydrodistillation from the aerial parts of Salvia aegyptiaca L. (Lamiaceae) growing wild in pre-Saharan region of Algeria, was analysed by GC-MS. Forty-one compounds were detected, representing 87.9% of the whole oil. The essential oil of S. aegyptiaca is characterized by the predominance of monoterpene derivatives (70.6%). The major constituents were tricyclene (22.9%), followed by limonene (17.5%), β-pinene (7.4%), caryophyllene oxide (3.2%) and β-caryophyllene (3.1%). Tricyclene, the main component in our analysis is generally absent in the volatile oils from other Salvia species, therefore, the Algerian accession of S. aegyptiaca may be categorized as a new chemo-type
Chemical composition and antioxidant activity of essential oil from daucus reboudii coss., an endemic plant of Algeria
No full textChemical constituents and anticholinesterase activity of the essential oil of Algerian Elaeoselinum thapsioides (desf.) maire
No full textElaeoselinum thapsioides is an Algerian medicinal plant used in traditional medicine to treat different diseases. The essential oil obtained by hydrodistillation from the aerial parts of Elaeoselinum thapsioides (Desf.) Maire (Apiaceae) growing wild in Algeria, was analyzed by GC-MS for the first time. Forty-five compounds were detected, accounting for 93.8% of the total oil, which was characterized by a high content of hydrocarbons derivatives of monoterpenes (75.9%). Myrcene (61.0%) was the principal constituent of the essential oil, followed by germacrene D (10.3%), α-pinene (6.5%) and β-pinene (2.9%). In vitro anticholinesterase activity of the essential oil was investigated by the Ellman method that evidenced a low acetylcholinesterase and butyrylcholinesterase inhibitory effect
Total phytochemical analysis of Thymus munbyanus subsp. coloratus from Algeria by HS-SPME-GC-MS, NMR and HPLC-MSn studies
No full textAntioxidant activity of extracts obtained by high-pressure extraction procedures from Asparagus stipularis Forssk
No full textRoots and rhizomes of Asparagus stipularis Forssk were separately extracted using supercritical CO2 extraction (SC–CO2) and pressurized liquid extraction (PLE) and their Total Phenols Content and antioxidant potential was investigated. The results indicated that the roots and rhizomes are a potential source of natural antioxidants. The PLE method gave better extraction yields than SFE and its fractions present the highest antioxidant activity. Direct evaluation of antioxidant capacity using the QUENCHER procedure showed that also the solid residue of the plant material remaining after the SC–CO2 and PLE may be of interest as a source of valuable phytochemicals
NMR, HS-SPME-GC-MS and HPLC-MSn analyses of phytoconstituents and aroma profile of Rosmarinus eriocalyx L
No full textIn this work, a comprehensive study on the chemical constituents of the aerial parts of Rosmarinus eriocalyx(Lamiaceae), an aromatic shrub traditionally consumed as a food and herbal remedy in Algeria, is presented. Thearoma profile was analysed by headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC/MS), whereas the crude extr act constituents were analyzed by1H-NMR and by highperformance liquid chromatography coupled with mass spectrometry (HPLC/MSn). Thirty-nine volatile compounds,most of them being monoterpenes, have been identified, with campho r, camphene, and a-pinene as the mostabundant constituents .1H-NMR analysis revealed the presence of phenolic compounds and betulinic acid whileHPLC/MSnallowed the identification of glycosilated and aglyconic flavonoids as well as phenylpropanoid derivatives.Some of these constituents, namely as betulinic acid, rosmanol, and cirsimaritin were reported for the first time inR. eriocalyx
Impact of Bovine Serum Albumin on the Antioxidant and Anti-Inflammatory Activities of Arbutus unedo L. Extract
No full textThis study explored the impact of bovine serum albumin (BSA) on the antioxidant and anti-inflammatory activities of Arbutus unedo leaf extract and its polyphenolic compounds. HPLC-MS analysis identified compounds like quinic acid, arbutin, and flavonoids. Antioxidant activity was evaluated using ferric reducing power, phosphomolybdenum, and DPPH assays, while anti-inflammatory effects were assessed via red blood cell membrane stability. BSA enhanced hydroxyl radical scavenging but reduced ferric reducing power and total antioxidant capacity, with no significant anti-inflammatory improvement. The findings reveal assay-dependent effects of BSA due to protein–polyphenol interactions, emphasizing the need for further research to optimize therapeutic potential
Chemical composition and antibacterial activity of essential oils from Daucus sahariensis Murb. roots and stems
No full textDaucus genus (Apiaceae) comprises economically relevant plants distributed in temperate regions. These plants are used mainly as food but are also known for their biological properties. Here, the essential oils (EOs) obtained by hydro-distillation of roots and stems of D.sahariensis Murb. growing in Algerian Sahara were studied by GC-MS analysis. Fifty-two and fifty-five compounds were identified from the roots and stems, representing 95.1% and 93.8% of the whole EOs respectively. The main constituents of the EO from roots were myristicin (29.2%), (E)-anethole (14.2%), cis-chrysanthenyl acetate (8%) and estragol (7.5%). In the EO from stems, the main constituents were α-pinene (18.6%), myrcene (16.7%), limonene (13.7%) and myristicin (8%). The antibacterial activity of EOs was evaluated by disc diffusion method and tested against Gram-positive and Gram-negative bacteria. Results showed a mild activity against Bacillus subtilis and Klebsiella pneumoniae for both EOs, which can be potentially used as antibacterial in phytotherapy
Composition and profiling of essential oil, volatile and crude extract constituents of Micromeria inodora growing in western Algeria
No full textThe chemical constituents of the aerial parts of Micromeria inodora (Desf.) Benth. (Lamiaceae) collected in western Algeria, an aromatic shrub traditionally consumed as an herbal remedy, have been characterized. Secondary metabolites were extracted using sequential extractions with solvents at different polarity, and they were determined using an integrated nuclear magnetic resonance (NMR) and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) approach. The composition of the essential oil obtained by hydrodistillation was characterized by gas chromatography-mass spectrometry (GC–MS), and the aroma profile was analysed by head-space solid phase micro extraction (HS-SPME) coupled with GC–MS. NMR and LC–MSn analyses revealed the presence of aglyconic and glycosylated flavonoids, phenylpropanoid derivatives and triterpenoid acids, mainly in the methanol, dichloromethane and n-hexane extracts. The analysis of the essential oil allowed to identify 66 components, mainly oxygenated mono- and sesquiterpenes (38.2 % and 32.0 %, respectively) and sesquiterpene hydrocarbons (10.8 %). The aroma profile revealed by HS-SPME-GC–MS was characterized by 45 volatile compounds, among which oxygenated monoterpenes appeared to be the most abundant (65.8 %), followed by sesquiterpene hydrocarbons (16.4 %) and monoterpene hydrocarbons (10.6 %). Overall, these data indicate that M. inodora could be a potential source of antioxidants and bioactive compounds, and they represent a starting point for further research on this plant species
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