1,203 research outputs found
Assessing Substrate Utilization and Bioconversion Efficiency of Black Soldier Fly (Hermetia illucens) Larvae: Effect of Diet Composition on Growth and Development Temperature
Black soldier fly larvae (BSFL) can utilize food by-products or residues for growth, benefiting farm animal’s diets’ production sustainability. The experiment aimed to assess the effect of different substrate compositions on larval growth, chemical composition, and substrate temperature. BSFL were allocated to one of the four diets (control, vegetable, carnivorous, and omnivorous) for the entire experiment (8 days). The temperature was measured twice daily using a thermal-imaging camera, and the accumulated degree hours (ADH) was calculated. The results showed that the larvae fed the vegetable diet exhibited a significantly reduced growth performance, with a biomass reduction of 26.3% compared to the control diet; furthermore, vegetable-fed larvae showed a lower dry matter content (−30% compared to the average of other diets) due to lower fat content (−65% compared to average of other diets). The nutritional composition of larvae fed an omnivorous diet was similar to larvae fed a high-quality substrate diet (control diet-chicken feed), indicating that the omnivorous diet could be an ideal solution for rearing BSFL larvae; however, the current European legislation prohibits the use of animal meal. The study also revealed that substrate temperatures did not have a discernible influence on larval growth, further emphasizing the importance of diet in BSFL rearing strategies
Black Soldier Fly Larvae’s Optimal Feed Intake and Rearing Density: A Welfare Perspective (Part II)
The large-scale insect rearing sector is expected to grow significantly in the next few years, with Hermetia illucens L. (black soldier fly, BSF) playing a pivotal role. As with traditional livestock, it is essential to improve and ensure BSF welfare. A starting point can be an adaptation of the Five Freedoms framework. Feed availability must be optimized to meet larvae nutritional needs (freedom from hunger) while maximizing substrate conversion efficiency. Similarly, rearing density needs to be optimized to ensure well-being, particularly in large-scale operations. In this study, Control (commercial laying hen feed) and Omnivorous substrates (vegetable and meat) were used as dietary regimes. In the first trial, three feeding rates were tested: 50, 100, and 200 mg feed/larva/day; in the second trial, three rearing densities were evaluated: 5, 10, and 15 larvae/cm2. Performance parameters, including final larval weight, final frass biomass, growth rate, substrate reduction, feed conversion ratio, larval length, survival rate, larvae chemical composition, and process optimization, were studied. Our results show that a feeding rate of approximately 90 mg feed/larva/day in the Omnivorous diet and 175 mg feed/larva/day in the Control diet, along with a rearing density of 5 and 7.57 larvae/cm2, respectively, in the Omnivorous and Control diets, produced optimal growth performances ensuring larval well-being. This outcome offers valuable insights for implementing good welfare practices in the insect farming sector and optimizing rearing management and efficiency
First person – Simona Amodeo
ABSTRACT
First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Simona Amodeo is the first author on ‘Characterization of the novel mitochondrial genome replication factor MiRF172 in Trypanosoma brucei’, published in Journal of Cell Science. Simona is a PhD student in the lab of Torsten Ochsenreiter at the Institute of Cell Biology, University of Bern, Switzerland, investigating mitochondrial genome anchoring, replication and inheritance in Trypanosoma brucei.</jats:p
Let Hermetia illucens free (from hunger and discomfort)! Welfare evaluation on larval stage.
Welfare evaluation is raising concerns about traditionally farmed animals, where 5 Freedom of Brambell’s report (5F) are applied. Despite no guidelines are present for insects yet, the International Platform of Insects for Food & Feed (IPIFF) recommends applying these principles. Insect welfare during their rearing can be indirectly evaluated considering performance parameters, as a suitability indicator of the conditions applied. Black soldier fly (Hermetia illucens L., BSF) is increasing in popularity as innovative feed and large-scale farming is expected to grow. This study determined the best dietary regime (T1), feeding rate (T2), rearing density (T3) for BSF larvae (L), integrating the 5F into the rearing process. Both small- (100 L in T1 and T2, 320 –960 L in T3) and large-scale (2000 L in T1 and T2, 3045 –9135 L in T3) experiments were set-up, with four replicates for each treatment. We evaluated performance parameters (growth rate, FCR, final larval weight, substrate reduction, survival rate) and BSF chemical composition. In T1, vegetarian (V), carnivorous (M), and omnivorous (O) diets was compared, with a control (C). Then, three feeding rates (T2: 50, 100, and 200 mg/day/larva) were evaluated, and compared rearing density values of 5, 10, and 15 larvae/cm2 (T3). The data were elaborated using one way ANOVA and Tukey HSD, or Kruskal-Wallis rank sum test and Dunn test. Even if the survival rate did not register significative differences, the omnivorous diet was the most efficient for BSF analyzing the growth parameters (growth rate +20% and +9% if compared to V and M diets, respectively). Optimized rearing can be achieved by applying a feeding rate of 100 mg/day/larvae (FCR -8% and -40% if compared with O50 and O200, respectively) and with density of 10 larvae/cm2 (final larval weight + 7% and growth rate +10% if compared with O15) ensuring both efficiency and the welfare of the insect. This holds a key role, especially from an industrial perspective, considering the future development of the sector
Effects of the administration on live larvae as enrichment material on weaned piglets behaviour
Black Soldier Fly (BSF) larvae could be used as a feed ingredient (larvae meal, LM) and as an enrichment material (LL, live larvae). In a study, LL improved piglets behaviour in an experimental setting (Ipema, 2021). We tested the effects of LL administration under conventional rearing conditions.
Forty-eight piglets (commercial hybrids, 28d old) receiving the same diet (containing 6% LM) were split into two experimental groups (4 replications of 6 piglets per group): C (control, kept in standard commercial conditions) and LL (receiving 300-600g/pen/day of BSF LL). The trial lasted 40 days. Diurnal behaviour was videotaped every 10 days and analysed (scan sampling technique) to obtain individual time budgets. Data were submitted to ANOVA using the group as fixed effect and the pig as random effect.
Time budgets were affected by live larvae administration, with LL piglets spending 13% of the observed time interacting with larvae, reducing time spent exploring the pen floor (3.5 vs. 5.1%, F(191)=6.6, P<0.05), and manipulating the traditional enrichment (metal chain and wood logs, 1.3 vs. 4.8%, F(191)=36.6, P<0.01). Social interactions were also lower in LL piglets (7.6 vs. 9.6%, F(191)=8.4, P<0.01). The overall activity level (time spent resting) was not affected (45.0 vs. 47.7%, F(191)=2.5, P>0.05).
Pending more extensive behavioural observations (in continuous behavioural analysis is in progress), our results indicate that BSF LL administration can improve piglets behaviour on farm by shifting the exploratory behaviour from the pen fixtures and traditional enrichments to larvae without increasing the animals’ activity levels
A First Step Towards Black Soldier Fly Larvae (Diptera: Stratiomyidae) Welfare by Considering Dietary Regimes (Part I)
The insect farming sector is expanding, but knowledge of insect welfare is still limited. This article aims to optimize the dietary regime for “black soldier fly” (Hermetia illucens L., BSF) larvae by applying a holistic view of welfare. Four diets were tested: control (CONTR, commercial laying hen feed), vegetable (VEG), omnivorous (OMN), and carnivorous (MEAT) diet, conducting experiments at a large (2000 larvae) and small scale (100 larvae). Rearing parameters were calculated including the growth rate, substrate reduction, efficiency of conversion of digested food, waste reduction index, and survival rate. Chemical analyses were conducted on BSF larvae and the residual frass. While the MEAT diet appears to be non-well-performing for the larvae, the VEG diet performed comparably to the control diet. Interestingly, the OMN diet demonstrated improved efficiency when evaluating the growth process at both scales. The chemical composition of larvae and frass highlighted the nutritional adequacy of the OMN diet, with the BSF larvae showing adequate protein and lipid content without nutrient catabolism or signs of discomfort. Applying the five freedoms of Brambell’s report as a welfare standard for animal rearing and evaluating performance as an indirect indicator of welfare, the OMN diet appears to promote larval welfare in rearing practices
Materiali e discussioni per l'analisi dei testi classici. Indici 1-60
Il volume raccoglie e riassume tutti gli articoli apparsi nei volumi 1-60 della rivista «Materiali e discussioni per l’analisi dei testi classici» («MD»), nel periodo compreso tra il 1978 e il 2008. Esso è diviso in tre parti. Nella prima sono riportati in successione i singoli numeri della rivista, con il relativo sommario. Nella seconda compaiono gli articoli riuniti in sequenza cronologica, sotto il nome dell’Autore e ognuno con un proprio numero d’ordine: di ogni articolo è fornito il sunto, con i concetti, le argomentazioni salienti, i principali luoghi discussi. Nella terza parte sono elencati gli Autori antichi e i Nomi e parole notevoli, con il rinvio al numero d’ordine dell’articolo. Le schedature dei numeri 1-30 sono curate da Andrea Cucchiarelli, quelle dei numeri 31-60 da Simona Fortini.The volume collects and summarizes all the articles published in Volumes 1-60 of the journal "Materiali e discussioni per l’analisi dei testi classici” ("MD"), in the period between 1978 and 2008. It is divided into three parts. In the first part the individual issues of the journal are summarized with the table of contents. In the second part the articles appear in chronological order, under the name of the author and each with its own serial number: each item is provided with a summary including the main points of argumentation and the loci discussed. The third part contains the indexes of the ancient authors, of the names and of the most relevant things and words, with reference to the number of the item. The profiling of the numbers 1-30 is cared for by Andrea Cucchiarelli, those of the numbers 31-60 by Simona Fortini
Boccaccio nel Seicento: censure e recuperi della "compassione"
SIMONA MORANDO, Boccaccio in the sixteenth century: censorship and recovery ofthe «compassion»
In the introduction of this paper, the literature of the seventeenth century is defined as a writing of compassion but not of consolation (quotations from Giovanni Cisano, Torquato Accetto, Emanuele Tesauro, G. B. Marino). The distance between the
writers studied here and Boccaccio is due to the censored editions of the Decameron in sixteenth century, to the censorship of Boccaccio as a «maestro d’amore», to his unrefined language, to his interpretation by many critics as a light and burlesque author. But, as reported in a writing by Boccalini, Boccaccio’s Latin works are very much appreciated in seventeenth century literature. This is especially the case of De casibus virorum illustrium, a gloomy and pessimistic book about misfortunes of famous men and about compassion, which nevertheless believes in the ability of letters to give back fame to ruined men. As shown in the conclusion
through some writings by Tassoni, many other seventeenth century authors do not have this kind of hope
Le chiavi del Paradiso. Primato petrino e devozione mariana di Sisto IV tra Cappella Sistina e S. Maria della Pace
Il volume è strutturato in due parti distinte con una postfazione, un indice dei nomi e n. 55 Tavole a colori fuori testo, valide per entrambe le parti del volume. I. PARTE (di Lorenzo Cappelletti) A partire dalla esplicita indicazione che l’ignoto architetto, collocato sul fianco destro dell’affresco sistino della Consegna delle chiavi di Perugino, fa dell’edificio a pianta centrale che campeggia sullo sfondo, viene operata una completa rilettura iconografica e iconologica dell’intero affresco. Tenendo conto di tutta la migliore bibliografia sul tema e grazie anche alla considerazione di fonti inedite o mai considerate in relazione a tale affresco –– vengono riletti in particolare i due episodi evangelici sullo sfondo, la teoria dei dodici apostoli e dei personaggi dell’attualità di fine Quattrocento e soprattutto il gesto della consegna delle chiavi a Simon Pietro da parte di Gesù.
La rilettura si allarga necessariamente anche alla Punizione di Core, Datan e Abiram, l’affresco di Botticelli posto in chiave tipologica dirimpetto alla Consegna delle chiavi, e alla serie dei 30/32 papi affrescati nel cleristorio.
Lo studio non manca di offrire inoltre spunti per una comprensione più adeguata anche degli altri affreschi quattrocenteschi della sistina. II PARTE (di Simona Benedetti) Nell’ambito della revisione di lettura iconologica e iconografica dell’affresco della Consegna delle chiavi di Perugino nella Cappella Sistina, svolta da Lorenzo Cappelletti, si torna a riflettere anche sul significato dell’architettura dell’edificio rappresentato al centro dell’affresco. In questo senso nel contributo di Simona Benedetti, in primo luogo, si ripercorrono i riferimenti alla trattatistica quattrocentesca in materia di edifici sacri, che possono aver influito nella determinazione dell’edificio ottagonale.
In seconda istanza, si considerano le principali fabbriche a pianta ottagonale costruite precedentemente all’edificio dipinto, che possono aver costituito un fondato riferimento per il Perugino nella Consegna delle chiavi.
In particolare tra le architetture, che possono avere avuto un’influenza determinante nella definizione iconografica della fabbrica ottagonale presente nell'affresco, la chiesa di S. Maria della Pace è quella su cui si concentra il presente studio. L'opera si realizzò nel cuore pulsante della Roma rinascimentale proprio contemporaneamente all’affresco in esame, con esso trova degli innegabili punti di tangenza e corrispondenze, sia storiche che figurative. A questo riguardo interessantissima risulta la connessione tra il motivo dell’edificazione della fabbrica e i fatti storici, i personaggi, gli eventi prodigiosi e le pratiche devozionali, che condizionarono lo svolgersi del cantiere del Templum Pacis negli ultimi anni del pontificato di Sisto IV. La storicità del gesto della consegna delle chiavi a Pietro - avvenimento che si protrae nel tempo ad ogni elezione pontificia nello spazio fisico della Cappella Sistina - costituisce la centralità del messaggio iconografico dell'affresco del Perugino in cui, anche l’architettura, insieme ai personaggi rappresentati, strutturano una narrazione reale, estranea alle simbologie decontestualizzate dall’epoca propriamente sistina. Segue una postfazione di Francesco Andreani dal titolo Enigma del Quattrocento, saggio nel quale si sviluppano considerazioni sulle personalità degli architetti e maestranze attive nella Roma del tardo Quattrocento e negli anni di inizio della fabbrica di S. Maria della Pace. Il volume si conclude con l'indice dei nomi (a cura di Gemma Fusciello)I (of Lorenzo Cappelletti) Starting from the unknown architect’s forefinger pointed to the central plan building found in the background of the Sistine Perugino’s fresco The Keys of the Kingdom of Heaven, the author offers a new interpretation of the entire fresco at both an iconographical and iconological level.
Having considered the most important studies about this fresco and taking into account a number of unpublished and unconsidered sources, the author rereads in particular the meaning of the central plan building, as well as the two Gospel episodes in the background; and he rereads also the grouping of the Twelve, along with the fifteenth century historical personages painted among them, in the foreground.
The author’s vision necessarily broodens also to comprehend Botticelli’s fresco named The Punishment of Corah, Dathan and Abiram which is placed on the opposite wall as a typological prefiguration of The Keys of the Kingdom of Heaven; and it broodens moreover to reconsider the series of 30/32 popes frescoed in the upper register of the Sistine Chapel.
The essay also offers hints for a more complete comprehension of the other fifteenth century frescoes of the Sistine Chapel.
II. (of Simona Benedetti) As part of the review of iconological and iconographic reading about the fresco of the “Delivery of the keys” by Perugino in the Sistine Chapel, conducted by Lorenzo Cappelletti, you go back and think about the significance of the building architecture depicted in the center of the fresco. In this sense, the contribution of Simona Benedetti, first, retraces the references to the fifteenth-century treatises concerning sacred buildings, which may have affected the determination of the octagonal building. Secondly, we consider the main factories octagonal building constructed previously painted, that may have been an established reference for the Perugino in the “Delivery of the keys”. Especially between architectures, which may have had a decisive influence in defining the iconographic factory octagonal present in the fresco, the church of St. Mary of Peace is the one on which this study focuses. The work was realized in the heart of Renaissance Rome just simultaneously fresco concerned, although it is undeniable points of contact and correspondence, both historical and figurative. In this regard are interesting connections between the reason of building the factory, the historical facts, the characters and the miraculous events and devotional, which has conditioned the unfolding of the construction site of the Templum Pacis in the last years of the pontificate of Sixtus IV. The historicity of the act of handing over the keys to Peter, that continues over time in the physical space of the Sistine Chapel, is the centrality of the iconographic message of the fresco by Perugino; so even the architecture, along with the characters represented, the real narrative structure, far from to the symbolism belonging to the Sistine period properly
Playing with autism. Encountering Simona Concaro by her music. Vol. 1.1
Playing with Autism contains twenty-four original pieces for piano, recorded and carefully transcribed in current music notation by Pierluigi Politi and Hanna Shybayeva. Simona Concaro, the author, is a young woman suffering of low-functioning autism. She lives in Cascina Rossago, a farm-community, designed to meet the needs of people with autism, in Oltrepo pavese (Italy).
Since she was three, Simona Concaro has created original compositions on piano, instrument she learned by herself, refusing any kind of musical education. In everyday life, Simona does not use verbal language and has low autonomy. Her music, on the contrary, contains interesting features – melodic, rhythmic and harmonic –, even in the presence of some iterative elements, typical of the autistic spectrum
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