24,085 research outputs found
Non-invasive detection and quantification of the parasitic ciliate Ichthyophthirius multifiliis by real-time PCR
No full textThe main parasitic threat to freshwater fish is the ciliate Ichthyophthirius multitihis. We developed a real-time PCR assay using SYBR Green (TM) intercalating fluorescent dye for rapid detection and quantification of I. multifiliis. This non-invasive assay was based on the quantification of L multitihis free-swimming stages from filtered water samples, and thus made it possible to preserve host individuals. An alignment of 18S rDNA sequences of I. multifiliis and related species of the ciliate order Hymenostomatida was used to design amplification primers specifically targeting the L multifillis 18S rDNA gene. Different standard curves consisting of 2-fold serial dilutions of DNA extracted from 20, 60, 100 and 1000 I. multifiliis cells were constructed. The assay was able to detect less than 0.5 cell equivalent and showed a strong linearity (R-2 = 0.984). Water samples were collected from 2 tanks containing heavily infected and apparently uninfected Carassius auratus specimens and were used to test this technique. Positive signals were obtained from water samples collected from both tanks, with a deduced concentration ranging from 3 to 58 L multifiliis cells 1(-1). The assay can detect low concentrations of the parasite in water, presumably corresponding to an early phase of the disease. It may, thus, be a valuable tool in assisting in the monitoring and control of ichthyophthiriasis in aquaculture
Die Qual der Wahl
No full textEin Gespräch mit Waiden Bello über den (Vor-) Wahlkampf, geführt am 26. Nov. 1997 mit Katharina Stahlenbrecher im philippinenbüro, Essen
Aportes al desarrollo turístico desde la UCAB: Aportes al desarrollo turístico desde la UCAB
No full textMy presentation is about how the Andrés Bello Catholic University is contributing to tourism. I want to highlight that previous speakers, as well as yesterday's speakers, have mentioned and justified the background that gave rise to this project. I am simply going to summarize it. The presentations by Mr. Jorge Roig and Gilbert Almarza confirm this.
At the Andrés Bello Catholic University, all its faculties and schools have not remained anchored in the past, we are in constant movement towards the search of specifying what businessmen require and what reality demands, not only national but international, in addition to offering satisfactory responses to the needs of our students and also what recent graduates of General Secondary and Technical Secondary Education aspire to.Mi ponencia trata de cómo está aportando la Universidad Católica Andrés Bello, al turismo, quiero resaltar que los ponentes anteriores, así como los de ayer han mencionado y justificado los antecedentes que dieron pie a este proyecto Sencillamente voy a resumirlo. Las ponencias del Señor Jorge Roig y de Gilbert Almarza así lo confirman.
En la Universidad Católica Andrés Bello todas sus facultades y escuelas no se han quedado anclados en el pasado, estamos en movimiento constante hacia la búsqueda de precisar lo que requieren los empresarios y lo que demanda la realidad, no solo nacional sino internacional, además de ofrecer respuestas satisfactorias a las necesidades de nuestros estudiantes y también qué aspiran los recién egresados de la Educación Media General y Media Técnica
Protecting Animals 36: Author Witi Ihimaera
No full textIn this very special episode of Knowing Animals I am joined by beloved New Zealand author Witi Ihimaera. Witi has written many books featuring nonhuman animals. He offers us a non-colonial lens through which to think about the human/nonhuman relationship
Solution structure of glutathione bound to human glutathione transferase P1-1: Comparison of NMR measurements with the crystal structure
No full textThe conformation of the bound glutathione (GSH) in the active site of the human glutathione transferase P1-1 (EC 2.5.1.18) has been studied by transferred NOE measurements and compared with those obtained by X-ray diffraction data. Two-dimensional TRNOESY and TRROESY experiments have been performed under fast-exchange conditions. The family of GSH conformers, compatible with TRNOE distance constraints, shows a backbone structure very similar to the crystal model. Interesting differences have been found in the side chain regions. After restrained energy minimization of a representative NMR conformer in the active site, the sulfur atom is not found in hydrogen-bonding distance of the hydroxyl group of Tyr 7. This situation is similar to the one observed in an "atypical" crystal complex grown at low pH and low temperature. The NMR conformers display also a poorly defined structure of the glutamyl moiety, and the presence of an unexpected intermolecular NOE could indicate a different interaction of this substrate portion with the G-site. The NMR data seem to provide a snapshot of GSH in a precomplex where the GSH glutamyl end is bound in a different fashion. The existence of this precomplex is supported by pre-steady-state kinetic experiments [Caccuri, A. M., Lo Bello, M., Nuccetelli, M., Nicotra, M., Rossi, P., Antonini, G., Federici, G., and Ricci, G. (1998) Biochemistry 37, 3028-3034] and preliminary time-resolved fluorescence data
Flexibility of helix 2 in the human glutathione transferase P1-1. time-resolved fluorescence spectroscopy
No full textTime-resolved fluorescence spectroscopy and site-directed mutagenesis have been used to probe the flexibility of alpha-helix 2 (residues 35-46) in the apo structure of the human glutathione transferase P1-1 (EC 2.5.1.18) as well as in the binary complex with the natural substrate glutathione. Trp-38, which resides on helix 2, has been exploited as an intrinsic fluorescent probe of the dynamics of this region. A Trp-28 mutant enzyme was studied in which the second tryptophan of glutathione transferase P1-1 is replaced by histidine. Time-resolved fluorescence data indicate that, in the absence of glutathione, the apoenzyme exists in at least two different families of conformational states. The first one (38% of the total population) corresponds to a number of slightly different conformations of helix 2, in which Trp-38 resides in a polar environment showing an average emission wavelength of 350 nm. The second one (62% of the total population) displays an emission centered at 320 nm, thus suggesting a quite apolar environment near Trp-38. The interconversion between these two conformations is much slower than 1 ns. In the presence of saturating glutathione concentrations, the equilibrium is shifted toward the apolar component, which is now 83% of the total population. The polar conformers, on the other hand, do not change their average decay lifetime, but the distribution becomes wider, indicating a slightly increased rigidity. These data suggest a central role of conformational transitions in the binding mechanism, and are consistent with NMR data (Nicotra, M., Paci, M., Sette, M., Oakley, A. J., Parker, M. W., Lo Bello, M., Caccuri, A. M., Federici, G., and Ricci, G. (1998) Biochemistry 37, 3020-3027) and pre-steady state kinetic experiments (Caccuri, A. M., Lo Bello, M., Nuccetelli, M., Nicotra, M., Rossi, P., Antonini, G., Federici, G., and Ricci, G. (1998) Biochemistry 37, 3028-3034) indicating the existence of a pre-complex in which GSH is not firmly bound to the active site
Desarrollo Sustentable Para Guayana, Los Retos Y Las Visiones Del Futuro
No full textVice Chancellor of the Andrés Bello Catholic University, Guayana campus, members of the organizing committee of the eighteenth Sustainable Guayana Forum, professors of the Andrés Bello Catholic University, Guayana campus, appreciated students attending the XVIII Sustainable Guayana Forum, ladies and gentlemen.For me, it is an honor and I am deeply humbled to be invited to deliver this keynote speech within the framework of the XVIII Sustainable Guayana Forum, a distinction that I assume with the greatest spirit of being useful and contributing to the praiseworthy work that has been carried out throughout of these encounters.On May 15, 2013, I took the floor at the thirteenth Sustainable Guayana forum to speak about the planetary limits that could condition the sustainable development of Guayana, in which I would refer to climate change, the nitrogen and phosphorus cycles, the loss of biodiversity , changes in land use and chemical contamination of water.In the face of climate change, we must comply with the mitigation measures that we assume in the planned national contributions as an expression of the will to abide by the terms signed in the 2016 Paris Agreement and prepare ourselves by adopting adaptation measures in the face of the inevitable effects of climate change. changeclimate; The other four limits must be included in the sustainable development strategies for Guayana to control them at tolerable values or eliminate them definitively.Señor Vicerrector de la Universidad Católica Andrés Bello sede Guayana, señores miembros del comité organizador del decimoctavo Foro Guayana Sustentable, señores profesores de la Universidad Católica Andrés Bello sede Guayana, apreciados estudiantes asistentes al XVIII Foro Guayana Sustentable, señoras y señores.Para mí es un honor que agradezco con profunda humildad que me hayan invitado a pronunciar esta conferencia magistral en el marco del XVIII Foro Guayana Sustentable, distinción que asumo con el mayor ánimo de ser útil y contribuir al loable trabajo que se viene realizando a lo largo de estos encuentros.El 15 de mayo de 2013 tomé la palabra en el decimotercer foro Guayana Sustentable para hablar sobre los límites planetarios que podrían condicionar el desarrollo sustentable de Guayana en los que me referiría al cambio climático, los ciclos de nitrógeno y fósforo, la pérdida de la biodiversidad, los cambios en el uso de la tierra y la contaminación química del agua.Ante el cambio climático debemos cumplir con las medidas de mitigación que asumimos en las contribuciones previstas nacionales como expresión de la voluntad de acatamiento de los términos suscritos en el Acuerdo de París de 2016 y prepararnos mediante la adopción de medidas de adaptación ante los efectos inevitables del cambioclimático; los otros cuatro límites debemos incluirlos en las estrategias de desarrollo sostenible para Guayana para controlarlos en valores tolerables o eliminarlos definitivamente
I Think I Am Philip K. Dick
No full textFor years, noted writer Laurence A. Rickels often found himself compared to novelist Philip K. Dickthough in fact Rickels had never read any of the science fiction writers work. When he finally read his first Philip K. Dick novel, while researching for his recent book The Devil Notebooks , it prompted a prolonged immersion in Dicks writing as well as a recognition of Rickelss own long-documented intellectual pursuits. The result of this engagement is I Think I Am: Philip K. Dick , a profound thought experiment that charts the wide relevance of the pulp sci-fi author and paranoid visionary. I Think I Am: Philip K. Dick explores the science fiction authors meditations on psychic reality and psychosis, Christian mysticism, Eastern religion, and modern spiritualism. Covering all of Dicks science fiction, Rickels corrects the lack of scholarly interest in the legendary Californian author and, ultimately, makes a compelling case for the philosophical and psychoanalytic significance of Philip K. Dicks popular and influential science fiction.Intro -- Contents -- Introjection -- Part I -- Endopsychic Allegories -- Schreber Guardian -- Belief System Surveillance -- Part II -- Deeper Problems -- Veil of Tears -- Go West -- Dick Manfred -- Timing -- Glimmung -- Part III -- Spiritualism Analogy -- Imitating the Dead -- Indexical Layer -- Ilse -- Hammers and Things -- Crucifictions -- Over There -- Martyrology -- Can't Live, Can't Live -- Lola -- Umwelt, Mitwelt, and Eigenwelt -- Outer Race -- The German Introject -- Part IV -- Materialism, Idealism, and Cybernetics -- Startling Stories -- A Couple of Years -- Android Empathy -- Homunculus and Robot -- ALL OF YOU ARE DEAD. I AM ALIVE. -- Go with the Flow -- Part V -- Room for Thought -- Caduceus -- Jump -- Still -- A Wake -- Spätwerk -- Let the Dead Be -- Play Bally -- Das Hund -- Notes -- BibliographyFor years, noted writer Laurence A. Rickels often found himself compared to novelist Philip K. Dickthough in fact Rickels had never read any of the science fiction writers work. When he finally read his first Philip K. Dick novel, while researching for his recent book The Devil Notebooks , it prompted a prolonged immersion in Dicks writing as well as a recognition of Rickelss own long-documented intellectual pursuits. The result of this engagement is I Think I Am: Philip K. Dick , a profound thought experiment that charts the wide relevance of the pulp sci-fi author and paranoid visionary. I Think I Am: Philip K. Dick explores the science fiction authors meditations on psychic reality and psychosis, Christian mysticism, Eastern religion, and modern spiritualism. Covering all of Dicks science fiction, Rickels corrects the lack of scholarly interest in the legendary Californian author and, ultimately, makes a compelling case for the philosophical and psychoanalytic significance of Philip K. Dicks popular and influential science fiction.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
The Interpreters: Ritual, Violence, and Social Regeneration in the Writing of Wole Soyinka
No full textA concern for social regeneration stands as the factor that animates Soyinka's life-long involvement in social and political activism, leading to hid incarceration for two years during the civil war, and his having to flee into exile during the period of Sani Abacha's dictatorship. Soyinka expresses this same concern for social regeneration in his writings, using difference metaphors. The focus of this work lies in the exploration of the articulations of social regeneration in the works of Wole Soyinka. The first past focuses on the dramatic works, and the argument of the author is that the metaphor adopted by Africa's foremost playwright in articulating his vision of social regeneration is that of ritual. Attention shifts in part two to Soyinka's two novels; and here, Bello goes to the roots of Yoruba metaphysics to fetch a metaphor which describes a creature with contradictory personality; which at once is committed to the regeneration of the social order while at the same time retaining a vindictive, vengeful nature
»Es ist unökonomisch und unpolitisch, aber es ist die Realität« - Gespräch mit Walden Bello
No full textNach dem Asian Social Movements Meeting am 14. August 2002 in Bangkok interviewten Oliver Pye und Wolfram Schaffar Waiden Bello, den Direktor von Focus on the Global South, für südostasien.
- …
