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    Sjogren's syndrome and Helicobacter pylori infection

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    Objective: To determine whether Helicobacter pylori infection is associated with primary Sjogren's syndrome. Patients and methods: Four patients with Sjogren's syndrome underwent endoscopy. Three H. pylori culture-positive patients were treated with triple therapy for eradication of infection. One patient was not infected and was not treated. Eradication was verified endoscopically and/or serologically 2 and 6 months after the end of therapy. The severity of xerophthalmia and xerostomia was evaluated clinically and by the Shirmer's test before, and 2 and 6 months after the end of therapy. Results: H. pylori was eradicated from the three infected patients. The signs and symptoms of Sjogren's syndrome improved in all three patients. The Shirmer's test confirmed amelioration. Conclusion: H. pylori may trigger or worsen the symptoms of Sjogren's syndrome

    Comparison between degree of histological gastritis and DNA fingerprints, cytotoxicity and adhesivity of Helicobacter pylori from different gastric sites

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    Thirty-six isolates of H. pylori from up to three gastric biopsy sites (antrum, corpus and fundus) from 13 patients in Italy with different degrees of histological gastritis were investigated. All strains were tested for motility, cytotoxicity and degree of adhesion, and were typed by analysis of ribosomal RNA gene patterns (ribopatterns). Seventeen different DNA types (ribotypes) were identified, with each patient possessing H. pylori of one or more unique types. Only two patients had identical H. pylori at three sites. Most patients had H. pylori with different ribotypes or subtypes, but nine strains were not typable. Five patients had the same strain colonizing two of the three sites and atypical strains were mostly from the antrum. A complex pattern of H. pylori colonization in the stomach of some individuals was evident and suggested multiple sources of infection. No consistent associations were detected between degree of gastritis and adherence, cytotoxicity and motility but a 2.56Kb rRNA gene fragment that had a higher frequency in strains associated with severe gastritis than mild gastritis, may provide a useful molecular marker for future pathogenicity studies
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