196,159 research outputs found
Proliferative response and antibody production against SA5K, a secretion protein of M. tuberculosis in healthy subjects and patients with tuberculosis
No full textOver the last few years proteins actively secreted in culture filtrates by Mycobacterium tuberculosis have been the object of extensive studies as they are considered immunodominant antigens able to induce a protective immunity in animal models. A new 8.3 kDa secretion protein released in culture filtrates by M. tuberculosis, M. bovis BCG, and a few other mycobacterial species has recently been described in our laboratory. The aim of the present study was to purify the protein from recombinant E. coli cells and to test its immunological potential in healthy subjects and tuberculous patients. The results obtained demonstrated that SA5K is able to induce in vitro proliferation of CD4 + T lymphocytes from healthy M. bovis BCG-vaccinated subjects, but not from patients with tuberculosis. The poor specificity displayed by the recombinant antigen when tested in Western blotting with sera obtained from healthy subjects and tuberculous patients, does not suggest, at least in the recombinant form, its employment for a serodiagnosis of tuberculosis
COMPLETE RELEASE OF ADENOSINE-DEAMINASE FROM MOUSE LYMPHOCYTES STABILIZED BY LOW-PH ACETATE
No full textIntorno a Batoni. Ragioni di un convegno
No full textL. Barroero ha organizzato il convegno e curato gli Atti. Contributi di G. Fusari (Brescia), C. Parretti (Viterbo), S. Benedetti (Dublino), C. Hornsby (Londra), C. Mazzarelli (Mendrisio), M.B. Failla (Torino), A.M. Suarez Huerta (Madrid), J. L. Sedl (Cleveland), J. Collins (New York), M. Ingendaay (Firenze), M. Bezzini (Roma), R. Balzarotti (La Spezia), M.B. Guerrieri Borsoi (Roma), S. Rolfi ozvald (Roma), S. Rudolph (Firenze), P. Sannucci (Roma), M.Bonino (Lucca)
Coquandite, Sb6+xO8+x(SO4)(OH)x(H2O)1-x (x = 0.3) from the Cetine mine, Tuscany, Italy: crystal structure and revision of the chemical formula
No full textUse of a recombinant strain of Mycobacterium avium expressing beta-galactosidase to evaluate the activities of antimycobacterial agents inside macrophages
No full textA reliable and low-cost method that enables rapid screening of the activity exerted by new antimicrobial agents on intracellularly growing Mycobacterium avium has been developed. To this aim, a recombinant (lacZ) strain of M. avium expressing the Escherichia call beta -galactosidase gene was used to evaluate, in murine macrophages, the susceptibility of M. avium to common antimycobacterial agents. beta -Galactosidase levels, measured in the presence of each of the antibiotics tested, were closely correlated with the number of CFU recovered from the M. avium lacZ strain-infected macrophages
Anthony M. Clark (édité par Edgar Peters Bowron), Pompeo Batoni. A complete catalogue of his Works with an Introductory Text. Oxford, Phaidon, 1985. 416 p., rel., 431 ill. dont 16 pl. coul.
No full textMérot Alain. Anthony M. Clark (édité par Edgar Peters Bowron), Pompeo Batoni. A complete catalogue of his Works with an Introductory Text. Oxford, Phaidon, 1985. 416 p., rel., 431 ill. dont 16 pl. coul.. In: Revue de l'Art, 1986, n°72. p. 92
Anthony M. Clark (édité par Edgar Peters Bowron), Pompeo Batoni. A complete catalogue of his Works with an Introductory Text. Oxford, Phaidon, 1985. 416 p., rel., 431 ill. dont 16 pl. coul.
No full textMérot Alain. Anthony M. Clark (édité par Edgar Peters Bowron), Pompeo Batoni. A complete catalogue of his Works with an Introductory Text. Oxford, Phaidon, 1985. 416 p., rel., 431 ill. dont 16 pl. coul.. In: Revue de l'Art, 1986, n°72. p. 92
Involvement of the Mycobacterium tuberculosis secreted antigen SA-5K in intracellular survival of recombinant Mycobacterium smegmatis
No full textA new protein (SA-5K) secreted in Culture filtrates by Mycobacterium bovis,Mycobacterium tuberculosis. and few other mycobacterial species was previously identified and purified in our laboratory. In order to evaluate the putative role of SA-5K during intracellular mycobacterial growth, in the present Study the SA-5K gene was cloned and expressed in Mycobacterium smegmatis, a rapid growing nonpathogenic mycobacterium which does not contain the gene for the protein. SA-5K expression in the THP-1 human macrophage cell line infected with the recombinant strain (M. smegmatis-pROL5K) was demonstrated by RT-PCR on RNA extracted from bacterial cells following 24 and 48 h of infection. Intracellular SA5K expression was associated with a higher cfu increase of M, smegmatis-pROL5K in comparison to the negative control strain (M. smegmatis recombinant for the cloning vector) (P = 0.01). No significant change in SA-5K synthesis by M. smegmatis-pROL5K was observed when the recombinant strain was grown in vitro in different stress conditions such as iron deprivation. pH 4.5. presence of nitric oxide or hydrogen peroxide. The results presented in this study suggest a possible role for SA-5K in intracellular survival of recombinant M. smegmatis, though the function of the protein remains unknown
Longitudinal study on the effect of oral hygiene measures on the salivary count of microbial species with cariogenic potential
No full textThe effect of oral hygiene education measures and professional tooth cleaning on the salivary levels of microbial species with high cariogenic potential (i.e. Streptococcus mutans, Lactobacillus spp. and Candida albicans) was evaluated at different time points. At time 0, high salivary carriage rates were recorded in the study group (n=30). Fifty percent of the subjects harbored all three species in their saliva, 27% harbored 2 species, and 23% only one species. At 3 months after oral hygiene measures, a statistically significant reduction was observed in salivary count of S. mutans and Lactobacillus spp. The percentage of subjects harboring all three species was also highly reduced, along with an overall improvement of clinical and risk factors parameters. At 8 months after oral hygiene measures, S. mutans and Lactobacillus spp. load was still statistically lower than that recorded at time 0, although an increment in bacterial load and a partial worsening of clinical and risk factors parameters were observed. S. mutans count in saliva inversely correlated with salivary pH, while it positively correlated with C. albicans salivary levels. The results obtained suggest that strengthening of the motivation and administration of oral hygiene instructions and professional tooth cleaning every 6-8 months, might be necessary to control salivary levels of cariogenic species
A fully automatic non-planar slicing algorithm for the additive manufacturing of complex geometries
No full textThe aim of this work is the implementation of a novel non-planar slicing algorithm, capable of combining traditional planar layers with non-planar ones. The geometry to be reconstructed will consist of an inner volume made of planar layers, and an outer shell made of non-planar layers. Both planar and non-planar layers are in turn constituted by an outer region, the shell, and an inner region, the infill. The outer non-planar shell guarantees an enhancement in the aesthetic properties and electro-mechanical behaviour of the printed objects, as well as a better adhesion when directly built onto a complex geometry. The algorithm was tested on three different three-dimensional models usable for aerospace, electronics, and biomedical applications showing robust results. An experimental validation was carried out by using a 5-axis robotic printer equipped with an extrusion-based printing unit. Different structures combining planar and non-planar paths were printed onto complex substrates, as well as into a bone defect that was completely regenerated following the proposed approach. The comparison between our method and the existing non-planar slicing methods revealed several advantages such as the capacity to generate both top and bottom non-planar surfaces, the possibility to manage complex concave/undercut structures, as well as the option to directly register the planned printing path on the receiving substrate in the working environment. To ensure proper and easy use, a graphical user interface was also created
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