225,151 research outputs found
Bath Historical Society Newsletter 1996
No full textBath Historical Society Newsletter Number 38 (March–April 1996) “A History of the Jewish Community of Bath” by Donald M. Povich, son of Morris S. Povich, a founding member of Beth Israel Congregation (originally, Base Isroall) in Bath, Maine. He was a founding member of the Bath Historical Society and served in leadership roles at Beth Israel Congregation.https://digitalcommons.usm.maine.edu/jud_weinbergfred/1003/thumbnail.jp
The particulate methane monooxygenase from Methylococcus capsulatus (Bath)
No full textThe isolation procedure for the pMMO complex has been optimised to obtain a high
specific activity enzyme from Methylococcus capsulatus (Bath). The enzyme is
comprised of the pMMO hydroxylase (pMMOH) consisting of polypeptides 47,26 and
23kDa molecular mass. In addition to this, a putative pMMO reductase (pMMOR) was
also found to be necessary to maintain propylene oxidising activity. This component
was found to consist of two polypeptides of approximately 63 and 8kDa. Preliminary Nterminal
sequence data of the large subunit ofpMMOR indicates that the sequence bears
70% similarity to the methanol dehydrogenase (MDH) from Methylococcus capsulatus
(Bath). Therefore, we tentatively propose that the" MDH can act as a reductase
component to the pMMOH.
The significance of this result prompted investigations into the previous published
proposals that electrons derived from the methanol oxidation reaction can be channelled
back into the methane oxidation reaction by the methanol dehydrogenase, independent
of NADH. Any effect of methanol to act as a reductant to pMMO in membrane
preparations was lost upon isolation of the pMMO complex, indicating the necessity to
maintain a fully functional methanol dehydrogenase (MDH) upon isolation. In addition
to this, the in vitro electron donors of pMMO, NADH and duroquinol were found to act
via distinct pathways of electron transfer (electron transport inhibitor studies).
Electron paramagnetic resonance (EPR) spectroscopy data provided evidence that the
copper in the active site of pMMO existed as a mononuclear copper (II) centre not a
trinuclear copper centre suggested by Chan and coworkers (Chan et al., 1993; Nguyen et
al., 1994, 1996a, 1996b, 1998). In addition to this preliminary data also indicates the
presence of an iron centre which is only EPR visible after reduction of the complex
suggesting the majority of iron in the complex is EPR invisible. The exact nature of this
iron centre is still unclear.
A structural study of the pMMO complex has also been undertaken using electron
microscopy studies in conjunction with single particle analysis. This allowed low
resolution projection maps of different views of the pMMO complex to be generated.
The complex appears to exist in a polymeric state of at least a dimer, possibly a tetramer
if the molecular weight analysis calculated by sedimentation equilibrium analysis is
taken into account.
This study has provided some insight into basic characteristics and the structure of a
duroquinol-driven pMMO complex and its interaction with other electron transfer
proteins
A place in words: twenty-five years of creative writing at Bath Spa University [designer]
No full text'A Place in Words' is a celebration of twenty-five years of Bath Spa University’s very successful creative writing programme, and the culmination of a “flash fiction” competition for novels of 25 words in length. This new anthology also features contributions by Naomi Alderman, David Almond, Susan Beale, Nathan Filer, Maggie Gee, Tessa Hadley and Philip Hensher
Disciplinary Thinking - Values: assessment tasks
No full textTitle: Disciplinary Thinking - Values: assessment tasks
Description: A handout describing a potential M-level assignment on values in HE teaching.
Theme: Values
Subject: HE - Education
Author: Colleen McKenna & Jane Hughes: HEDERA, 2012
Audience: Educational developers within accredited programmes and courses in higher education
Issue Date: 05/07/2012
Last updated Date: 02/08/2012
Version: Final
PSF Mapping: A1, A4, A5, K1, PV1, PV2, PV4
License: Creative Commons Attribution-ShareAlike 2.0 UK: England & Wales License. (CC-BY-SA 2.0)
Keywords: ukoer, education, discthink, disciplinary thinking, hedera, university of bath, academic practice, values, ethics,
Produced for the Disciplinary Thinking Project at the University of Bath, as part of the Higher Education Academy/JISC Open Educational Resources (OER) Phase Three Programme: Embedding and Sustaining Change.
http://disciplinarythinking.wordpress.com
Virgaline Lewis Interview - Part 1 (Bath County)
No full textAn interview with Virgaline Lewis of Owingsville, Kentucky by Robert M. Rennick on the history and origin of community names in Bath County, Kentucky
Virgaline Lewis Interview - Part 2 (Bath County)
No full textAn interview with Virgaline Lewis of Owingsville, Kentucky by Robert M. Rennick on the history and origin of community names in Bath County, Kentucky
Shakespeare in Georgian Bath
No full textStudents from Bath Spa University’s College of Liberal Arts present 'A Shakespeare Ode' by Bath’s own musical genius, Thomas Linley junior, alongside a miniature opera, 'Thomas and Sally' by Thomas Arne, the composer associated with Garrick’s revival of the bard in Georgian England. Matthew Spring was the Artistic Director and Conductor for this historically researched concert in the Bath Music Festival 2016. The Vauxhall Players, Bath Spa Chamber Choir and Bath Spa University graduates performed
Commercial Issues in Private International law: A Common Law Perspective by M Douglas, V Bath, M Keyes and A Dickinson
No full textReview(s) of: 'Commercial issues in private international law: A common law perspective', by Michael Douglas, Vivienne Bath, Mary Keyes and Andrew Dickinson (eds), (Hart Publishing, 2019) ISBN 9781509922871 Hardback, 346 PP
Bath-applied PDF and sNPF induce cAMP increases in the IPCs.
No full textLive optical imaging in flies expressing a cAMP sensor in their IPCs (dilp2(p)>Epac1camps). Average inverse FRET traces (CFP/YFP) in IPCs reflecting intracellular changes in cAMP levels. Substances were bath-applied to freshly dissected fly brains at 100 s (indicated by black arrow). Application of 10−5 M adenylate cyclase activator NKH477 (NKH, dark gray) induced a robust increase in cAMP, indicating that the general procedure was working. As a negative control, hemolymph-like saline (HL3) was applied. (A) Bath-applied PDF (10−5 M) evokes cAMP increases in the IPCs (light blue), suggesting a possible functional connection between PDF+ cells and IPCs. Similar increase was observed when PDF was applied in the presence of 2μM sodium channel blocker tetrodotoxin (TTX; dark blue), indicating a direct connection. (B) Maximum inverse FRET changes quantified for each individual neuron and averaged for each pharmacological treatment between 100–1000 s. (C) A close-up of the immediate changes in cAMP levels occurring from the application point until 200 s. No significant changes can be observed when PDF or PDF+TTX were applied. (D) Maximum inverse FRET changes from 100–200 s. (E) Bath-applied sNPF (10−5 M) generates cAMP rises in the IPCs (yellow). Similar increase occurs in the presence of TTX (orange), thus suggesting a direct connection. (F) Maximum inverse FRET changes between 100–1000 s. (G) Magnification of the immediate changes between 100–200 s. (H) Maximum inverse FRET changes between 100–200 s. The legend shows the color code of the different treatments and the number of neurons in the dissected brains (in parentheses) considered in this analysis. Data are shown as mean ± SEM. Kruskal-Wallis test followed by Bonferroni-corrected Wilcoxon pairwise-comparisons. ***p<0.001, **p<0.01, *p<0.05, n.s. not significant.</p
Finite difference modelling of acoustic propagation and its applications in underwater acoustics
No full textSIGLEAvailable from British Library Document Supply Centre- DSC:DXN058285 / BLDSC - British Library Document Supply CentreGBUnited Kingdo
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