77 research outputs found
Magnanimous Kunti by Samaresh Basu/ সমরেশ বসুর কলমে মনস্বিনী কুন্তী
No full textSamaresh Basu wrote a number of books based on Puran-Mahabharata under the pseudonym Bhramar and Kalkoot. Such as, ‘Shamba (1978)’, ‘Juddher Sesh Senapati (1984)’, ‘Prachetas (1984)’, \u27Pritha (1986)\u27, ‘Antim Pranay (1987)’ etc. ‘Pritha’ was published in the magazine \u27Prasad\u27 under the pseudonym \u27Bhramar\u27. In such books, the author analyzed the traditional story of the Puranas in a new perspective.
One of the memorable Panchakanyas in Puranas, Empress Kunti has been recreated in the light of the author\u27s spirit in this book. In the present article we will discuss how the character of Kunti has been recreated by Samaresh Basu in \u27Pritha\u27.
At the beginning of the story, before reaching the context of Kunti, the author undertakes a very realistic analysis of heaven-hell, Gods-demons, Samhita era-Puranic era, male-female relationship, marriage customs, child birth and the position of women in society. Then he explained the solitude, self-immolation and transition of Kunti from a feminist perspective.
The story of love-marriage-motherhood-heroism-restraint-pain-sacrifice of this remarkable female character of Mahabharata has been captured in a new way in the unique writing of Kalkoot. Inventing many thoughtful arguments the author tried to establish the father-son relationship between Yudhisthira-Vidura and Karna-Durbasha. How the author incarnated new contexts in the familiar story of Mahabharata and how he made it acceptable by arranging relevant arguments in favour of his new thoughts – this essay will try to elaborate these points
Binding of the putative anticancer agent chelerythrine to double stranded poly(A): Calorimetry and spectral characterization studies
No full textThe benzophenanthridine plant alkaloid chelerythrine was recently reported to bind to single stranded
polyriboadenylic acid [ss poly(A)] through an entropy driven process with remarkably high binding affinity
(�107 M�1) (Basu and Suresh Kumar, 2015). Considering the anticancer effects of chelerythrine along with
its potential to be developed as an RNA targeted drug due to its high affinity to ss poly(A), here we studied
the binding of chelerythrine to double stranded (ds) poly(A). The binding was characterized thermodynamically
by enthalpy and entropy changes, and enthalpy-entropy compensation behaviour. The binding thermally stabilized the structure. The binding led to hypochromic and bathochromic effects in the visible absorption spectrum of chelerythrine and enhanced its fluorescence intensity. An intercalative binding mode was deduced from fluorescence quenching, anisotropy, and viscometric studies. The binding of chelerythrine to the ds poly(A) was significantly weaker than its binding to ss poly(A). The results may lead to designing RNA targeted therapeutics and a useful agent in gene regulation in eukaryotic cells
ANALISA PEMAHAMAN ABK DAN BURUH PADA SAAT PENGOPERASIAN PENUTUP PALKA TIPE PONTOON DI MV. PRITHA
No full textFachrudin, 2017, NIT: 50134784. N,"Analysis of Crew and Worker
Understanding on Operating Hatch Cover Type Pontoon in MV.
Pritha”, Nautical Thesis, Diploma IV Program, Semarang
Merchant Marine Polytechnic, Material Adviser (I): Capt.I Kadek
Laju, S.H., M.M., M.Mar. and as Methodologi and Writing
Adviser (II): Nur Rohmah, S.E., M.M.
Pontoon is a block-shaped iron plate with hollow inside and placed
transversely on hatch threshold which function to protect the loads. Hatch cover
type pontoon is opened and closed manually with vessel’s derick boom. The
operation of opening and closing hatch cover takes excessive time and energy, as
pontoon has to be lifted one by one and placed above other unopened pontoon.
The operating nature of hatch cover type pontoon has a huge risk, such as in Port
of Bayah, 23rd of June 2016, at 14.00 Indonesia Western Time, a worker who was
on duty got hit by pontoon. Therefore, analysis on the impact of lack of crew
understanding on the operation of hatch cover type pontoon is needed. The
objective of this research is to find out the impact of the lack of crew
understanding on operation of hatch cover type pontoon. Moreover, this research
also aim to find out the attempt to increase the crew’s and worker’s discipline in
using safety equipment to operate hatch cover type pontoon.
This research uses descriptive and qualitative method to specifically
describe the operation of hatch cover type pontoon which lead to work accident in
MV Pritha. In determining the priority of the problem, the author uses USG
method (Urgency, Seriousness, Growth) to give score from the sale 1 to 5 in the
causes of work accident in MV Pritha.
The research result indicates that the impacts of the lack of crew
understanding in operating hatch cover type pontoon in MV. Pritha are the
increase of work accident risk when the operation of hatch cover type pontoon
takes place, the failure of equipment used in operating hatch cover type pontoon,
the lack of time and energy efficiency in operating hatch cover type pontoon.
Steps needed to take to increase the discipline of crew and worker on using safety
equipment in operating hatch cover type pontoon are giving instruction onboard
on the importance to use safety equipment in operating hatch cover type pontoon,
conducting safety meeting associated with discipline on personal safety equipment
usage in operating hatch cover type pontoon.
Keywords : Crew, worker, hatch cover type pontoo
Biophysical studies on the interaction of the alkaloid chelerythrine with nucleic acids
No full textDNA-deoxyribonucleic acid is the blueprint for life. It is present in organisms ranging from
the smallest bacterium to the largest whale. DNA carries most of the genetic instructions
used in the development, functioning and reproduction of well known living organisms.
James Dewey Watson and Francis Harry Compton Crick had revolutionized the field of
molecular biology and medicine by proposing the structure of deoxyribonucleic acid through
model building studies in their celebrated paper published in the Nature magazine of April
23 (Watson and Crick, 1953). Although the Watson and Crick was known as father of DNA the
preliminary research on DNA was started many decades ago on 1868 by Swiss chemist
Friedrich Miescher. Miescher in 1868 detected a phosphorus-containing substance from the
nuclei of pus cells obtained from discarded surgical bandages. He named it ‘nuclein’ consisting
of an acidic portion which we know today as DNA. In 1878, Albrecht Kossel isolated the nonprotein
component of “nuclein”, the nucleic acid, and later isolated its five primary
nucleobases (Albrect, 1879). In 1919, Phoebus Levene identified the base, sugar and phosphate
nucleotide unit (Levene, 1919). Levene suggested that DNA consisted of a string of nucleotide
units linked together through the phosphate groups. Levene thought the chain was short and
the bases repeated in a fixed order. In 1937, William Astbury produced the first X-ray diffraction
pattern that showed that DNA had a regular structure (Astbury and Florence, 1938). In 1944
Oswald Avery and his coworkers discovered that DNA carries a cell’s genetic material and
can be altered through transformatio
Biophysical Studies on the Interaction of the Akaloid Chelerythrine with Nucleic Acids
No full textDNA-deoxyribonucleic acid is the blueprint for life. It is present in organisms ranging from the smallest bacterium to the largest whale. DNA carries most of the genetic instructions used in the development, functioning and reproduction of well known living organisms.
James Dewey Watson and Francis Harry Compton Crick had revolutionized the field of molecular biology and medicine by proposing the structure of deoxyribonucleic acid through model building studies in their celebrated paper published in the Nature magazine of April
23 (Watson and Crick, 1953). Although the Watson and Crick was known as father of DNA the preliminary research on DNA was started many decades ago on 1868 by Swiss chemist Friedrich Miescher. Miescher in 1868 detected a phosphorus-containing substance from the nuclei of pus cells obtained from discarded surgical bandages. He named it ‘nuclein’ consisting of an acidic portion which we know today as DNA. In 1878, Albrecht Kossel isolated the nonprotein component of “nuclein”, the nucleic acid, and later isolated its five primary nucleobases (Albrect, 1879). In 1919, Phoebus Levene identified the base, sugar and phosphate nucleotide unit (Levene, 1919). Levene suggested that DNA consisted of a string of nucleotide
units linked together through the phosphate groups. Levene thought the chain was short and the bases repeated in a fixed order. In 1937, William Astbury produced the first X-ray diffraction pattern that showed that DNA had a regular structure (Astbury and Florence, 1938). In 1944
Oswald Avery and his coworkers discovered that DNA carries a cell’s genetic material and can be altered through transformation
Implementation of a new homeownership program in Jakarta: The case of zero down-payment housing program
No full textGovernments worldwide deal with the question of how to elevate their low-income household (LIH) into a higher standard of living. The main view of scholars on housing is that homeownership usually is the first step into improving the lives of people with a low income. The Province of Jakarta struggled with the same question. To address the housing challenge in Jakarta, they created the Zero Down-Payment Housing Program (ZDPHP) in 2017. This program's main purpose is to provide financing facilities to meet the basic need for adequate housing, targeting the LIH. However, the adaptation of this program has been slow, begging the question of what could be done to attract more beneficiaries. This article is intended to help improve the ZDPHP in Jakarta and give a perspective for other places thinking about implementing the same program. It explores the current eligibility criteria from the perspective of two out of five elements of the "5 As of Adequate Housing", Affordability and Accessibility, to understand the underlying housing challenge. Indicators of those two elements were collected from the data associated with the project. These data sources were supported and further explained using empirical data from policies analysis, interviews, FGDs, surveys and other existing databases. The most important findings were found as follows. Firstly, the best proportion of housing expenses to household income is to look at the actual comparison between household income and what a household pays for housing and transportation in its respective city. Secondly, to further strengthen the housing planning in Jakarta, an education program should be enforced alongside the ZDPHP. Lastly, with the recent launch of regulation regarding government land use permits, it is hoped that this will be a key solution to overcome land limitations and reduce house prices to become more accessible and affordable.Architecture, Urbanism and Building Sciences | Management in the Built Environmen
Hypoaspis takiensis Bhattacharyya 1966
No full textHypoaspis takiensis Bhattacharyya, 1966 Hypoaspis takiensis.— Ghosh, 2002: 38. Hypoaspis takiensis.— Ghosh & Mandal, 2017: 4. Collection records in India: Zoological Park of Darjeeling Himalayas, West Bengal, in soil (Ghosh & Mandal, 2017). Notes: Ghosh & Mandal (2017) referred to a species named Hypoaspis takiensis Bhattacharyya, 1966. We have been unable to locate the description of any species by that name, described by Bhattacharyya or any other author. Many species of mites have been described in PhD or Masters theses, which are produced in a very limited number of copies and which are not generally available for distribution. The names of these species are therefore nomina nuda. We refer to one example, by Datta (1991), but there are many others. Datta (1991) described and illustrated five new species of Laelapidae from India, including four species of Hypoaspis and one species of Pseudoparasitus, but these names were not published in a way that makes them available for nomenclatural purposes.Published as part of Bandyopadhyay, Pritha, Karmakar, Krishna & Halliday, Bruce, 2023, Checklist of Indian mites in the family Laelapidae (Acari: Mesostigmata), pp. 401-424 in Zootaxa 5249 (4) on page 416, DOI: 10.11646/zootaxa.5249.4.1, http://zenodo.org/record/769457
A comparative study on the interaction of the putative anticancer alkaloids, sanguinarine and chelerythrine, with single- and double-stranded, and heat-denatured DNAs
No full textA detailed investigation on the interaction of two benzophenanthridine alkaloids, sanguinarine (SGR) and chelerythrine (CHL), with the double-stranded (ds), heat-denatured (hd), and single-stranded (ss) DNA was performed by spectroscopy and calorimetry techniques. Binding to the three DNA conformations leads to quenching of fluorescence of SGR and enhancement in the fluorescence of CHL. The binding was cooperative for both of the alkaloids with all the three DNA conformations. The binding constant values of both alkaloids with the ds DNA were in the order of 106 M−1; binding was weak with hd and much weaker to the ss DNA. The fluorescence emission of the alkaloid molecules bound to the ds and hd DNAs was quenched much less compared to those bound to the ss DNA based on competition with the
anionic quencher KI. For both double stranded and heat denatured structures the emission of the bound alkaloid
molecules was polarized significantly and strong energy transfer from the DNA bases to the alkaloid molecules occurred. Intercalation of SGR and CHL to ds, hd, and ss DNA was proved from these fluorescence results. Calorimetric studies suggested that the binding to all DNA conformations was both enthalpy and entropy favored. Both the alkaloids preferred double-helical regions for binding, but SGR was a stronger binder than CHL to all the three DNA structures
Elucidation of the DNA binding specificity of the natural plant alkaloid chelerythrine: A biophysical approach
No full textInteraction of the anticancer plant alkaloid chelerythrine with four sequence specific synthetic polynucleotides
was studied by spectroscopy and calorimetry experiments. The binding resulted in strong hypochromic and bathochromic effects in the absorption spectrum of the alkaloid, enhancement in the fluorescence with the AT polynucleotides and the homo-GC polynucleotide and quenching with the hetero- GC polynucleotide. Cooperative binding was observed with all the polynucleotides. Fluorescence polarization anisotropy, iodide quenching and viscosity results confirmed intercalative binding of the alkaloid. The binding resulted in the thermal stabilization of the polynucleotides and moderate perturbations in the B-conformation of the DNA. The high binding affinity values (�106 M�1) evaluated from the spectroscopic data was in excellent agreement with those obtained from calorimetry. The binding was exothermic and favoured by negative standard molar enthalpy and positive standard molar entropic contributions in all cases other than homo-AT polynucleotide, where it was endothermic and entropy driven.
Salt-dependent calorimetry data revealed that the binding reaction was driven mostly by non-polyelectrolytic
forces. The magnitude of the negative heat capacity values confirmed the role of significant hydrophobic effects in the interaction profile of the alkaloid with the polynucleotides. The results revealed the specificity of chelerythrine to follow homo-GC > hetero-GC > hetero-AT = homo-AT polynucleotid
Structural and thermodynamic basis of interaction of the putative anticancer agent chelerythrine with single, double and triple-stranded RNAs
No full textcomparative study on the interaction of the natural plant alkaloid chelerythrine with triple helical poly(U)poly(U) and single stranded poly(U) (the dot and star representing the
Watson-Crick and Hoogsteen base pairing) has been performed using various biophysical and thermodynamic techniques. Chelerythrine binds to the duplex and triplexes in a cooperative manner with affinity of the order of 106 M�1. A weaker binding (�105 M�1) in a non-cooperative mode occurred
with poly(U). Chelerythrine is more selective towards RNA triplex than its parent duplex. The triplex was stabilized specifically without affecting the stability of the duplex. Fluorescence quenching, fluorescence polarization and energy transfer from the nucleotides to the alkaloid, and viscosity results gave convincing evidence for a true intercalative binding of chelerythrine to the triplex and the duplex structures, and partial base stacking with poly(U). The conformations of both double and triple helices
were perturbed on binding but no effect occurred to the single strand structure. The binding of the alkaloid to all three RNA helices was found to be exothermic; to the triplex it was entropy driven with favorable enthalpy change, to the duplex enthalpy driven and to the single strand it was enthalpy driven.These results provide new knowledge on the mode, mechanism and specificity, and energetics of binding of the natural alkaloid and putative anticancer agent chelerythrine to different RNA conformation
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