33,926 research outputs found
Cytosolic proteins from tobacco pollen tubes that crosslink microtubules and actin filaments in vitro are metabolic enzymes
No full textIn plant cells, many processes require cooperative action of both microtubules and actin filaments, but proteins mediating interactions between these cytoskeletal members are mostly undiscovered. Here, we attempt to identify such proteins by affinity purification. Cytosol from Nicotiana tabacum (tobacco) pollen tubes was incubated first with actin filaments, and then proteins eluted from the actin were incubated with microtubules, and finally those microtubule-binding proteins were pooled in an active fraction. This fraction bundled actin filaments but not microtubules. However, when the fraction was added to both actin and microtubules, large bundles resulted, containing both polymers, regardless of the order of addition of components. Similar results were obtained when the order of affinity purification was reversed. The four most abundant bands from the fractions were identified from peptide fragments analyzed by mass spectrometry. The same four proteins were identified regardless of the order of affinity purification. The proteins are: homocysteine methyltransferase, phosphofructokinase, pyruvate decarboxylase, and glucan protein synthase (reversibly glycosylated protein). These results suggest the importance of structuring metabolism within the confines of the pollen tube cytoplasm
Sport, inclusion et innovation : le cas italien du "Baskin" (2001-2013)
No full textBaskin (inclusive Basketball) was born in Italy in the early 2000s to allow the joint participation of so-called "able-bodied" players and players with disabilities. By focusing on the emergence of this new sport, this study aims to understand how well the sports logic can co-exist with the inclusive logic. The process of social innovation, as represented by the evolution of Baskin in Italy from 2001 to 2013, provides a privileged key for reading this study, which addresses the conflict arising from the tendency of sport to generate selection and homogeneity and the tendency of inclusion to value heterogeneity. To what extent does Baskin represent a new cultural model in sport and design an original form of social interaction ? Can it be considered an innovative democratic fiction situated in this paradoxical in-between which reconciles heterogeneity and competition and avoids at the same time the twin pitfalls of standardization and charitable welfarism ? This dual identity, sport and inclusiveness, gives Baskin strenght but also fragility. That's why the development of this new sport reveals an open and uncertain process of many parts : first the educational engineering of Baskin according to the principles of Design for all, then its delicate process of diffusion into the social environment, and finally the first steps of institutionalization of this new practice, with the aim of ultimately incorporating it into the shared cultural heritage. An ethnological investigation was carried out in a effort to reconstruct significant fragments of this "unique" case, through a transdiciplinary approachEn se penchant sur l'émergence d'un nouveau sport appelé le Baskin (Basket inclusif), né en Italie au début des années 2000 dans le but de permettre la participation conjointe de joueurs dits "valides" et de joueurs ayant une déficience, l'étude entend contribuer à comprendre dans quelle mesure et de quelle manière la logique sportive et la logique inclusive peuvent cohabiter. C'est le processus d'innovation sociale que représente l'itinéraire du Baskin en Italie de 2001 à 2013 que la recherche retient comme clé de lecture privilégiée pour aborder ce rapprochement problématique entre la tendance sélective du sport à créer de l'homogénéité, et la tendance inclusive à valoriser l'hétérogénéité. Le Baskin incarne-t-il dans le champ sportif un nouveau modèle culturel, une forme de sociabilité originale, une fiction démocratique innovante se situant dans cet entre-deux paradoxal, conciliant hétérogénéité et compétition, et évitant en même temps le double écueil de la normalisation et de l'assistanat ? Fort et fragile à la fois de cette double identité, sportive et inclusive, le développement du Baskin révèle un processus ouvert et incertain, où se succèdent le processus d'ingénierie pédagogique d'un sport dont l'architecture est construite selon les principes de la conception universelle, les processus délicats d'appropriation de cette invention par le milieu social, enfin l'orientation vers l'institutionnalisation de cette nouvelle pratique afin de la faire éventuellement accéder au patrimoine culturel commun. une enquête immersive est développée en tâchant de restituer quelques fragments significatifs sur ce cas inédit au travers d'un ancrage transdisciplinair
Sport, inclusion et innovation : le cas italien du "Baskin" (2001-2013)
No full textBaskin (inclusive Basketball) was born in Italy in the early 2000s to allow the joint participation of so-called "able-bodied" players and players with disabilities. By focusing on the emergence of this new sport, this study aims to understand how well the sports logic can co-exist with the inclusive logic. The process of social innovation, as represented by the evolution of Baskin in Italy from 2001 to 2013, provides a privileged key for reading this study, which addresses the conflict arising from the tendency of sport to generate selection and homogeneity and the tendency of inclusion to value heterogeneity. To what extent does Baskin represent a new cultural model in sport and design an original form of social interaction ? Can it be considered an innovative democratic fiction situated in this paradoxical in-between which reconciles heterogeneity and competition and avoids at the same time the twin pitfalls of standardization and charitable welfarism ? This dual identity, sport and inclusiveness, gives Baskin strenght but also fragility. That's why the development of this new sport reveals an open and uncertain process of many parts : first the educational engineering of Baskin according to the principles of Design for all, then its delicate process of diffusion into the social environment, and finally the first steps of institutionalization of this new practice, with the aim of ultimately incorporating it into the shared cultural heritage. An ethnological investigation was carried out in a effort to reconstruct significant fragments of this "unique" case, through a transdiciplinary approachEn se penchant sur l'émergence d'un nouveau sport appelé le Baskin (Basket inclusif), né en Italie au début des années 2000 dans le but de permettre la participation conjointe de joueurs dits "valides" et de joueurs ayant une déficience, l'étude entend contribuer à comprendre dans quelle mesure et de quelle manière la logique sportive et la logique inclusive peuvent cohabiter. C'est le processus d'innovation sociale que représente l'itinéraire du Baskin en Italie de 2001 à 2013 que la recherche retient comme clé de lecture privilégiée pour aborder ce rapprochement problématique entre la tendance sélective du sport à créer de l'homogénéité, et la tendance inclusive à valoriser l'hétérogénéité. Le Baskin incarne-t-il dans le champ sportif un nouveau modèle culturel, une forme de sociabilité originale, une fiction démocratique innovante se situant dans cet entre-deux paradoxal, conciliant hétérogénéité et compétition, et évitant en même temps le double écueil de la normalisation et de l'assistanat ? Fort et fragile à la fois de cette double identité, sportive et inclusive, le développement du Baskin révèle un processus ouvert et incertain, où se succèdent le processus d'ingénierie pédagogique d'un sport dont l'architecture est construite selon les principes de la conception universelle, les processus délicats d'appropriation de cette invention par le milieu social, enfin l'orientation vers l'institutionnalisation de cette nouvelle pratique afin de la faire éventuellement accéder au patrimoine culturel commun. une enquête immersive est développée en tâchant de restituer quelques fragments significatifs sur ce cas inédit au travers d'un ancrage transdisciplinair
Sport, inclusion et innovation : le cas italien du "Baskin" (2001-2013)
No full textBaskin (inclusive Basketball) was born in Italy in the early 2000s to allow the joint participation of so-called "able-bodied" players and players with disabilities. By focusing on the emergence of this new sport, this study aims to understand how well the sports logic can co-exist with the inclusive logic. The process of social innovation, as represented by the evolution of Baskin in Italy from 2001 to 2013, provides a privileged key for reading this study, which addresses the conflict arising from the tendency of sport to generate selection and homogeneity and the tendency of inclusion to value heterogeneity. To what extent does Baskin represent a new cultural model in sport and design an original form of social interaction ? Can it be considered an innovative democratic fiction situated in this paradoxical in-between which reconciles heterogeneity and competition and avoids at the same time the twin pitfalls of standardization and charitable welfarism ? This dual identity, sport and inclusiveness, gives Baskin strenght but also fragility. That's why the development of this new sport reveals an open and uncertain process of many parts : first the educational engineering of Baskin according to the principles of Design for all, then its delicate process of diffusion into the social environment, and finally the first steps of institutionalization of this new practice, with the aim of ultimately incorporating it into the shared cultural heritage. An ethnological investigation was carried out in a effort to reconstruct significant fragments of this "unique" case, through a transdiciplinary approachEn se penchant sur l'émergence d'un nouveau sport appelé le Baskin (Basket inclusif), né en Italie au début des années 2000 dans le but de permettre la participation conjointe de joueurs dits "valides" et de joueurs ayant une déficience, l'étude entend contribuer à comprendre dans quelle mesure et de quelle manière la logique sportive et la logique inclusive peuvent cohabiter. C'est le processus d'innovation sociale que représente l'itinéraire du Baskin en Italie de 2001 à 2013 que la recherche retient comme clé de lecture privilégiée pour aborder ce rapprochement problématique entre la tendance sélective du sport à créer de l'homogénéité, et la tendance inclusive à valoriser l'hétérogénéité. Le Baskin incarne-t-il dans le champ sportif un nouveau modèle culturel, une forme de sociabilité originale, une fiction démocratique innovante se situant dans cet entre-deux paradoxal, conciliant hétérogénéité et compétition, et évitant en même temps le double écueil de la normalisation et de l'assistanat ? Fort et fragile à la fois de cette double identité, sportive et inclusive, le développement du Baskin révèle un processus ouvert et incertain, où se succèdent le processus d'ingénierie pédagogique d'un sport dont l'architecture est construite selon les principes de la conception universelle, les processus délicats d'appropriation de cette invention par le milieu social, enfin l'orientation vers l'institutionnalisation de cette nouvelle pratique afin de la faire éventuellement accéder au patrimoine culturel commun. une enquête immersive est développée en tâchant de restituer quelques fragments significatifs sur ce cas inédit au travers d'un ancrage transdisciplinair
Exploiting knowledge of immune selection in HIV-1 to detect HIV-specific CD8 T-cell responses
No full textSince HLA-restricted cytotoxic T-cell responses select specific polymorphisms in HIV-1 sequences and HLA diversity is relatively static in human populations, we investigated the use of peptide epitopes based on sites of HLA-associated adaptation in HIV-1 sequences to stimulate and detect T-cell responses ex vivo. These "HLA-optimised" peptides captured more HIV-1 Nef-specific responses compared with overlapping peptides of a single consensus sequence, in interferon-γ enzyme linked immunospot assays. Sites of immune selection can reveal more immunogenic epitopes in HLA-diverse populations and offer insights into the nature of HLA-epitope targeting, which could be applied in vaccine design
Heterogeneous and tissue-specific regulation of effector T cell responses by IFN-gamma during Plasmodium berghei ANKA infection.
No full textIFN-γ and T cells are both required for the development of experimental cerebral malaria during Plasmodium berghei ANKA infection. Surprisingly, however, the role of IFN-γ in shaping the effector CD4(+) and CD8(+) T cell response during this infection has not been examined in detail. To address this, we have compared the effector T cell responses in wild-type and IFN-γ(-/-) mice during P. berghei ANKA infection. The expansion of splenic CD4(+) and CD8(+) T cells during P. berghei ANKA infection was unaffected by the absence of IFN-γ, but the contraction phase of the T cell response was significantly attenuated. Splenic T cell activation and effector function were essentially normal in IFN-γ(-/-) mice; however, the migration to, and accumulation of, effector CD4(+) and CD8(+) T cells in the lung, liver, and brain was altered in IFN-γ(-/-) mice. Interestingly, activation and accumulation of T cells in various nonlymphoid organs was differently affected by lack of IFN-γ, suggesting that IFN-γ influences T cell effector function to varying levels in different anatomical locations. Importantly, control of splenic T cell numbers during P. berghei ANKA infection depended on active IFN-γ-dependent environmental signals--leading to T cell apoptosis--rather than upon intrinsic alterations in T cell programming. To our knowledge, this is the first study to fully investigate the role of IFN-γ in modulating T cell function during P. berghei ANKA infection and reveals that IFN-γ is required for efficient contraction of the pool of activated T cells
Long-term wind-driven X-ray spectral variability of NGC 1365 with Swift
No full textWe present long-term (months–years) X-ray spectral variability of the Seyfert 1.8 galaxy NGC 1365 as observed by Swift, which provides well-sampled observations over a much longer time-scale (six years) and a much larger flux range than is afforded by other observatories. At very low luminosities, the spectrum is very soft, becoming rapidly harder as the luminosity increases and then, above a particular luminosity, softening again. At a given flux level, the scatter in hardness ratio is not very large, meaning that the spectral shape is largely determined by the luminosity. The spectra were therefore summed in luminosity bins and fitted with a variety of models. The best-fitting model consists of two power laws, one unabsorbed and another, more luminous, which is absorbed. In this model, we find a range of intrinsic 0.5–10.0 keV luminosities of approximately 1.1–3.5 erg s?1, and a very large range of absorbing columns, of approximately 1022–1024 cm?2. Interestingly, we find that the absorbing column decreases with increasing luminosity, but that this result is not due to changes in ionization. We suggest that these observations might be interpreted in terms of a wind model in which the launch radius varies as a function of ionizing flux and disc temperature and therefore moves out with increasing accretion rate, i.e. increasing X-ray luminosity. Thus, depending on the inclination angle of the disc relative to the observer, the absorbing column may decrease as the accretion rate goes up. The weaker, unabsorbed, component may be a scattered component from the wind
DNA fusion gene vaccination mobilizes effective anti-leukemic cytotoxic T lymphocytes from a tolerized repertoire
No full textThe majority of known human tumor-associated antigens derive from non-mutated self proteins. T cell tolerance, essential to prevent autoimmunity, must therefore be cautiously circumvented to generate cytotoxic T cell responses against these targets. Our strategy uses DNA fusion vaccines to activate high levels of peptide-specific CTL. Key foreign sequences from tetanus toxin activate tolerance-breaking CD4+ T cell help. Candidate MHC class Ibinding tumor peptide sequences are fused to the C terminus for optimal processing and presentation. To model performance against a leukemia-associated antigen in a tolerized setting, we constructed a fusion vaccine encoding an immunodominant CTL epitopederived from Friend murine leukemia virus gag protein (FMuLVgag) and vaccinated tolerant FMuLVgag-transgenic (gag-Tg) mice. Vaccination with the construct induced epitopespecificIFN-c-producing CD8+ T cells in normal and gag-Tg mice. The frequency and avidity of activated cells were reduced in gag-Tg mice, and no autoimmune injury resulted. However, these CD8+ T cells did exhibit gag-specific cytotoxicity in vitro and in vivo. Also, epitope-specific CTL killed FBL-3 leukemia cells expressing endogenous FMuLVgag antigen and protected against leukemia challenge in vivo. These results demonstrate a simple strategy to engage anti-microbial T cell help to activate epitope-specific polyclonal CD8+ T cell responses from a residual tolerized repertoire
Trip account
No full textTrip account - AMs, 15 pp.
“I am attempting to give you some account of a recent vacation trip which we were privileged to enjoy - Rose, Mother and I…” As the account of the trip to view the eclipse is unsigned, we can’t say for sure but as the author states “Rose, Mother and I” one could logically assume that the author is a sibling of T. Rose Curtis
Bianchi type-I universe in f(R, T) modified gravity with quark matter and Λ
No full text32nd International Physics Congress of Turkish-Physical-Society (TPS) -- SEP 06-09, 2016 -- Bodrum, TURKEYIn this study, we investigate homogeneous and anisotropic Bianchi type I universe in the presence of quark matter source in f (R, T) gravity (Harko et al. in Phys. Rev. D 84:024020, 2011) with cosmological constant A (where R is the Ricci scalar and T is the trace of the energy momentum tensor). For this aim we have used the anisotropy feature of Bianchi type I universe and equation of states (EoS) of quark matter. We explore the exact solution f(R, T)=R + 2f(T) model for Bianchi type I universe model. When t -> infinity, we get very small cosmological constant value, this result agrees with recent observations.Turkish Phys So
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