161 research outputs found
Biomimetic air-liquid interface milli-bioreactor for skin tissue engineering applications
No full textSelf-standing gelatin- methacryloyl 3D structure using Carbopol-embedded printing
No full textGelatin-methacryloyl (GelMA) hydrogel has gained huge success in the lastdecades thanks to its versatilities in many applications. Notably, one of them is3D bioprinting, as GelMA physical-mechanical properties and biocompatibilityof uncured formulation perfectly suit the requirements of a bioink. Neverthe-less, before the photopolymerization, the hydrogel shows weak mechanicalproperties and long recovery time after stress application, which results in theinability to obtain complex and self-standing forms due to structure collapse.In this work, Carbopol ETD 2020 NF, dissolved in cell culture medium, wasused as supporting bath to optimize GelMA bioprinting and overcome its sta-bility limitations. The achieved results demonstrated the possibility of printingshapes containing hollows with lumens or non-planar surfaces, also by usingnozzles with larger inner diameter, which reduced cell death during printingprocess, but were usually avoid because of low resolution. Moreover, con-structs' extraction was easier when Carbopol solution was prepared in culturemedium rather than in water, reducing sample handling. In conclusion,thanks to this supporting bath, it was possible to print cellularized scaffold,with channels that were then seeded, obtaining inner structure. Further, thisCarbopol formulation could be considered an eligible candidate as a support-ing bath to obtain GelMA 3D self-standing-shaped and vascularized scaffold
Printability study by selective laser sintering of bio-based samples obtained by using PBS as polymeric matrix
No full textThe emerging request to reduce the environmental impact of plastics encourages scientists to use novel sustainable
polymeric materials for many applications fields.
The present paper aims to use for the first-time poly (butylene succinate) (PBS), a biodegradable and compostable
polymer, for Selective Laser Sintering (SLS) applications. PBS is a flexible semicrystalline aliphatic
polyester, which can represent a very good alternative to the traditional thermoplastic polymers obtained by
fossil sources.
The present work started from a lab-scale production of PBS powders by means of an emulsion solvent
evaporation/precipitation method, with the purpose to increase the number of polymeric powders available for
SLS. The obtained PBS powders were first characterized by morphological and thermal point of view, and then
employed as innovative polymeric material in SLS to realized 3D printed parts with increasing geometrical
complexity. To confirm PBS cytocompatibility, cell proliferation and cell viability assays (MTT and Live&Dead)
were measured using a lung adenocarcinoma epithelial cell line (H1299). The in vitro cytotoxicity of the 3D
printed material was also investigated, showing no harm on cells
Implied Author, Overall Consideration, and Subtext of "Desiree's Baby"
No full textThis essay explores how to infer from a text the image of the implied author. It examines Kate Chopin's "Desiree's Baby" (1893), which has been widely regarded as an indictment of racism but which an "overall consideration" of the implied author's choices will lead us to see as a racist text. Through the interaction of various details in the text, the implied author suggests three racist dichotomies: (1) white characters' nondiscrimination versus black characters' discrimination, (2) positive slavery under white masters versus negative slavery under a black master, and (3) superior whites versus inferior blacks. This implied racist stance reflects the historical context of Chopin's personal experiences, but it contrasts with the quite different racial stances of the implied authors of some other Chopin narratives with different thematic designs. The complexity of the narratives under the name "Kate Chopin" offers an opportunity not only to gain a better understanding of the concept of implied author but also to clarify the relations (connections as well as disparities) among textual, intertextual, and extratextual evidence in literary interpretation in general.LiteratureA&HCI4ARTICLE2285-3113
GelMA synthesis and sources comparison for 3D multimaterial bioprinting
No full textGelatin Methacryloyl (GelMA) is one of the most used biomaterials for a wide range of applications, such as drug delivery, disease modeling and tissue regeneration. GelMA is obtained from gelatin, which can be derived from different sources (e.g., bovine skin, and porcine skin), through substitution of reactive amine and hydroxyl groups with methacrylic anhydride (MAA). The degree of functionalization (DoF) can be tuned by varying the MAA amount used; thus, different protocols, with different reaction efficiency, have been developed, using various alkaline buffers (e.g., phosphate-buffered saline, DPBS, or carbonate-bicarbonate solution). Obviously, DoF modulation has an impact on the final GelMA properties, so a deep investigation on the features of the obtained hydrogel must be carried on. The purpose of this study is to investigate how different gelatin sources and synthesis methods affect GelMA properties, as literature lacks direct and systematic comparisons between these parameters, especially between synthesis methods. The final aim is to facilitate the choice of the source or synthesis method according to the needs of the desired application. Hence, chemical and physical properties of GelMA formulations were assessed, determining the DoFs, mechanical and viscoelastic properties by rheological analysis, water absorption by swelling capacity and enzymatic degradation rates. Biological tests with lung adenocarcinoma cells (A549) were performed. Moreover, since 3D bioprinting is a rapidly evolving technology thanks to the possibility of precise deposition of cell-laden biomaterials (bioinks) to mimic the 3D structures of several tissues, the potential of different GelMA formulations as bioinks have been tested with a multi-material approach, revealing its printability and versatility in various applications
BIOMIMETIC BIOREACTOR FOR AIR-LIQUID INTERFACE CULTURE - SKIN TISSUE ENGINEERING APPLICATION
No full textMethodology for Comparing Viscoelastic and Poroelastic Properties of GelMA Hydrogels at the Cellular Scale
No full textSoundscape and sense of presence in the VR experience of Madame Pirate: Becoming a legend
No full textThis article proposes a phenomenological and critical analysis of the film, Madame Pirate: Becoming a Legend, written and directed by Morgan Ommer and Huang Dan-Chi (Taiwan: Serendipity Films 綺影映畫, 2022, VR360, 17 min), with the goal of encouraging more comparative analyses of narrative paradigms expressed through virtual reality. Particular attention will be paid to the sound aspect in Madame Pirate, which is foundational in establishing a sense of presence for the viewer. Following a discussion of theoretical framing for virtual reality production, the author presents an experiential report of her experience of Madame Pirate, focusing on how sound influences the sense of presence, directs the viewer’s gaze, and steers the narrative
Brownsville and Savage Rapids dam removal effectiveness monitoring
No full textprepared by: Desiree Tullos, Ph.D., PE.This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references (pages 31-33).OWEB #208-931Mode of access: Internet from the Oregon Government Publications Collection.Text in English
Non c’è pace tra gli ulivi. La sperimentazione inesausta del diritto penale dell’impresa e dell’economia. Spunti per un Confronto di idee
No full textL’Autore propone alcuni temi per un Confronto di idee in relazione al diritto dell’impresa e dell’economia, divenuto un “laboratorio” sperimentale a ciclo continuo delle riforme in ambito penale. Accanto alle fattispecie di nuovo conio ed alla modifica di quelle previgenti, viene dilatata l’estensione delle misure ablative e della responsabilità degli enti, mentre si confermano le strategie di contrasto alla corruzione e la necessità di individuazione anticipata dello stato di crisi delle imprese, nella cornice degli obiettivi connessi ai paradigmi dello sviluppo sostenibileThe author suggests a comparison of ideas in relation to business and economic law, which has become a continuous cycle experimental "laboratory" of reforms in the penal field. Alongside the newly minted cases and the alteration of the former ones, the extension of ablative measures and the prosecution and punishment of corporate Criminality is being expanded, while the strategies to combat corruption and the need for early identification of the state of corporate crisis are confirmed, in the frame of the sustainable development
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