102,688 research outputs found
Extracellular vesicles shedded by mouse mesoangioblasts have paracrine effects on different cell types
No full textIntroduction
Mouse mesoangioblasts are vessel associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. We have already demonstrated that these stem cells, as all the other stem cells, are able to release in the extracellular milieu membrane vesicles (EV) containing biological active molecules, such as FGF2, MMP2/91 and Hsp702. Today takes hold the idea that the vesicles can replace stem cells opening a new scenario in regenerative medicine3. To this aim, we investigated the possible paracrine interaction of mesoangioblast EV on different cell types and their effects.
Results and conclusions
We have showed that mesoangioblast EV interact with human endothelial cells by in vitro inducing their differentiation versus capillary-like structures, and increasing their migration capability. In addition, we have analyzed the immunomodulatory effect of EV on human lymphocytes. We have demonstrated that EV are able to inhibit both lymphocyte activation and proliferation.
Finally, we started to investigate the mechanisms of interaction between EV and target cells. In particular, we have observed the involvement of EV saccharidic residues in cell targeting. The enzymatic removal of EV N-lynked glycans by PNGase F, which cleaves between asparagine and GlcNAc in all types of glycan chains, induces a substantial reduction in EV-target cell interaction. Conversely, EndoH that is responsible for the cleavage between two residues of GlcNAc increases this interaction. In conclusion, saccharidic residues exert a role in EV-cell interplay.
References
1 Candela ME et al. J Cell Physiol 2010, 224: 144-51.
2 Barreca MM et al. J Cell Physiol. 2017, 232: 1845-61.
3 Tetta C et al. Endocrine 2013, 44: 11-19
Coastal retreat and marine flooding scenario for 2100: A case study along the coast of Maddalena peninsula (southeastern Sicily)
No full textANZIDEI M., SCICCHITANO G., TARASCIO S., DE GUIDI G., MONACO C., BARRECA G., MAZZA G., SERPELLONI E. & VECCHIO A., Coastal retreat and marine flooding scenario for 2100: a case study along the coast of Maddalena Peninsula (southeastern Sicily). (IT ISSN 0391-9838, 2018). The coastal area of southeastern Sicily (Italy) is undergoing weak land subsidence, heavy coastal retreat, land flooding and exposed to severe storms associated with high-waves, also in consequence of the global sea level rise, which is expected to raise even more that 1 m by 2100 AD depending on different estimates. This value will be even larger in subsiding coasts, entailing widespread environmental changes, coastal retreat, marine flooding and loss of land, which will be subtracted to human activities. To understand the impact of rising sea level on the coast of Maddalena Peninsula, near the town of Siracusa, we realized a very high resolution Digital Terrain Model (DTM) through aerial photogrammetric surveys, obtained by Unmanned Aerial Vehicles (UAV) on which we projected the expected coastline for 2100 AD. Here we show a detailed marine flooding scenario for 2100, as generated from: i) high resolution DTM, ii) rate of land subsidence from GPS data and iii) predicted sea level projections from the IPCC AR5 reports (RCP2.6 and RCP8.5). Our analysis estimates a maximum relative sea level rise at 0.20 m and 0.65 m for 2050 AD and 2100 AD, respectively for AR 8.5 scenario. The increased sea levels will cause relevant morphological changes to the investigated coast with a maximum beach retreat of 27 m and a loss of land of 7400 m2, affecting building integrity and people safety
Fondo Mario Dal Pra
No full textCon la pubblicazione dell’inventario del Fondo Mario dal Pra si prosegue l’opera di valorizzazione dei materiali archivistici conservati nella Biblioteca del Dipartimento di Filosofia dell’Università degli Studi di Milano. L’iniziativa riveste un particolare significato, in quanto il professor Dal Pra ha insegnato in questa università per trent’anni e ne è stato una delle figure più rappresentative. Le carte ed i quaderni di appunti di Dal Pra sono state donate all’Università nel 1992 dalla moglie Ines Rizzoli; esse erano già dotate di un ordinamento interno, attribuito dall’autore. In particolare, erano state approntate una serie di cartelle, indicanti i titoli dei materiali raccolti: sia che questi fossero suoi appunti manoscritti o dattiloscritti, sia che si trattasse di materiale per l’attività universitaria didattica o istituzionale. In seguito, per iniziativa della professoressa Maria Assunta Del Torre, le carte hanno avuto una prima, provvisoria, sistemazione. Negli ultimi tempi si è evidenziata l’esigenza di un loro riordino completo e definitivo, al fine di consentirne la consultazione. Oltre alle carte, Dal Pra aveva in precedenza donato all’Università i volumi di contenuto filosofico da lui posseduti (attualmente riuniti nel Fondo librario Mario Dal Pra, composto da 5000 volumi). Essi integrano in maniera significativa l’Archivio del filosofo
, a bempedoic acid metabolite
No full textX-ray powder diffraction data, including unit cell parameters and space group assignment, for the ESP15228 species of C19H34O5 formula, are here reported [a = 6.0434(6), b = 12.2543(6), c = 14.0285(8) angstrom, alpha = 86.584(3), beta = 85.707(10), gamma = 78.801(5)degrees, V = 1015.2(1) angstrom(3), Z = 2, rho(calc) = 1.152 g cm(-3), and space group P-1]. All measured lines were indexed and no detectable impurities were observed
Synthesis of Pyrazoles by 1,3-Dipolar Cycloaddition under Aqueous Micellar Catalysis
No full textEthyl diazoacetate (EDA), which is easily prepared from ethyl glycinate and NaNO2, reacts in situ with alkynes in a water micelle environment without organic solvent to form pyrazoles. The reaction is pH dependent, as in the presence of protic catalysis (H2SO4 4%, pH 3.5) a mixture of 3,5- and 4,5-disubstituted pyrazoles was obtained, while, at pH 5.5, only the 3,5-disubstituted isomer was obtained. The presence of the surfactant TPGS-750-M was crucial to secure clean crude reaction mixtures and high yields of the products. The same protocol was successfully applied to the synthesis of substituted pyrazolines. © 2022 The Author
Comment on the paper by Barreca et al.: “The Strait of Messina: Seismotectonics and the source of the 1908 earthquake” (Earth-Science Reviews 218, 2021, 103685)
No full textWe discuss the new causative source model for the 1908 Messina Straits earthquake recently proposed by Barreca et al. (2021), where an aseismic slip of 1.13 m along a low-angle discontinuity, preceding the 1908 earthquake, have mechanically destabilized a set of overlying faults, therefore leading them to the rupture. The lack of significant variations of the relative sea level in the Messina harbor area, in the time period relevant for the levelling data (1907–1908) analyzed by Barreca et al., and at least for the decade preceding the event proves the inconsistency of the assumed pre-earthquake aseismic slip. A careful interpretation of crustal earthquake distribution in the Strait does not support the presence of the low-angle discontinuity. The modelled horizontal coseismic pattern reveals a scenario that is not supported by any other independent geological and geophysical observation. We conclude that the source model proposed by Barreca et al. for the 1908 Messina Straits earthquake can not be considered as a viable hypothesis for the causative fault
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