38 research outputs found
Evaluation of polymerization stress correlated with the extent of polymerization of flowable composites.
No full textInfluence of different fixation reagents on NIH 3T3 fibroblasts morphology: An AFM study
No full textThe Atomic force microscope (AFM) has recently become an attractive tool for biological analysis, especially for the characterization of both morphological and mechanical properties. In this study, we investigated the influence of different fixation reagents on NIH 3T3 fibroblast cells morphology. Three protocols were selected: paraformaldehyde, methanol and acetone. Height, perimeter, area, volume and roughness of cells were measured using an AFM. As expected, different fixation protocols produce different morphological changes in cells. However, to preserve fibroblastsí
morphology, the methods applying aldehyde fixations performed much better than those using either methanol or acetone. Based on quantitative assessments, fibroblasts fixed with paraformaldehyde showed the closest volume, area and perimeter values to living cells. Roughness data and a Western blot test confirmed that methanol fixation produces larger cell membrane damage, when compared to the other two fixatives
Atomic force microscopy of 3T3 and SW-13 cell lines: an investigation of cell elasticity changes due to fixation
No full textMechanical properties of single cells are of increasing interest both from a fundamental cell biological perspective and in the context of disease diagnostics. In this respect, atomic force microscopy (AFM) has become a powerful tool for imaging and assessing mechanical properties of biological samples. However, while these tests are typically carried out on chemically fixed cells, the most important data is that on living cells. The present study applies AFM technique to assess the Young’s modulus of two cells lines: mouse embryonic fibroblasts (NIH/3T3) and human epithelial cancer cells (SW-13). Both living cells and those fixed with paraformaldehyde were investigated. This analysis quantifies the difference between Young’s modulus for these two conditions and provides a coefficient to relate them. Knowing the relation between Young modulus of living and fixed cells, allows carrying out and comparing data obtained during steady-state measurements on fixed cells that are more frequently available in the clinical and research settings and simpler to maintain and probe
A new BioMEMS for the study of mechanosensitive ion channels
No full textA novel completely transparent bioMEMS (bio - Micro Electro Mechanical System) has been designed and produced using finite element analysis (FEA) and micro-fabrication techniques. This device has been thought to be used for testing the mechanical properties of single living cells. Our bioMEMS is versatile and can be coupled to other analysis techniques and being completely transparent can be used with either transmission or reflection microscopes. This device is based on a silicon dioxide – silicon nitride structure
Mechanical Properties of 3T3 Fibroblasts due to Fixation Assessed Using Atomic Force Microscopy
No full textIn this paper we analyzed how the Mechanical Properties of 3T3 Fibroblasts change due to Fixation procedure. This was assessed using Atomic Force Microscop
AFM Cells Morphological Analysis: Influence of Different Environmental Conditions on Fixed and Living Cell
No full textIn this paper we carried out an AFM study on cells morphology, in particular we analyzed the Influence of Different EnvironmentalConditions on Fixed and Living Ce
