171,955 research outputs found
Antonio Banfi interprete di Nietzsche
No full textSi trata di uno studio sulle lezioni tenute da Antonio Banfi nell'anno accademico 1933-34, all'Università di Milano, su Friedrich Nietzsche. Il testo fu raccolto da uno studente e pubblcato per la prima volta nel 1974. Lo studio rileva i limiti di affidabilità del testo e le motivazioni contestuali della sua pubblicazione. Si cerca inoltre di mettere in rilievo le fonti di Banfi
Data for proteomic analysis of Human monocyte-derived macrophages
No full textThis data article is referred to the research article entitled Human monocyte-derived macrophages are heterogeneous: proteomic profile of different phenotypes by Eligini et al. Eligini S., Brioschi M., Fiorelli S., Tremoli E., Banfi C., Colli S. Human monocyte-derived macrophages are heterogeneous: proteomic profile of different phenotypes. J. Proteomics 124, 2015, 112-123. Macrophages obtained in vitro from blood monocytes are largely used as surrogate model of tissue macrophages that are heterogeneous and not easy to obtain and handle. Under spontaneous differentiation in vitro, monocyte-derived macrophages (MDMs) display two dominant subsets (round and spindle) that show different transcriptional, antigenic, and functional profiles mimicking, at least in part, the heterogeneity of tissue macrophages. This article reports the nano-LC-MSE analysis of the proteome of round and spindle MDMs allowing a deeper comprehension of macrophage heterogeneity
Extended reality and informative models for the architectural heritage: from scan-to-BIM process to virtual and augmented reality
No full text[EN] The dissemination of the tangible and intangible values of heritage building represents one of the most important objectives in the field of Digital Cultural Heritage (DCH). In recent years, different studies and research applied to heritage monuments have shown how it is possible to improve the awareness of the architectural heritage through the integration of latest developments in the field of 3D survey, 3D modelling, Building Information Modeling (BIM) and eXtended Reality (XR). On the other hand, this digital workflow requires a huge amount of data sources and a holistic approach to reach a high level of information sharing coming from different disciplines and sectors such as restoration, geomatics, 3D virtual museums and serious gaming. In conjunction with entertainment software and gaming, this research shows the main results obtained during the generative process of digital environments oriented to improve the level of information and to enrich the contents coming from the informative models. The case study is represented by one of the most important Lombard monuments: the Basilica of Sant’Ambrogio in Milan. This study, starting from the 3D survey and the data collection of the historical records of the church, improves the creation of an XR experience that reaches a new level of interactivity for different types of devices (desktop, mobile, VR headset) and users (experts, non-experts).Highlights:Generative modelling requirements and novel grades of generations (GOG) and accuracy (GOA) are presented in order to improve the digitisation of built heritage from the 3D survey, reducing time and costs of the scan-to-BIM process.The holistic value of generative modelling allows experts to create digital worlds able to faithfully and accurately represent the detected reality and improve new immersive environments for Virtual Reality (VR) and Augmented Reality (AR) projects.Immersive environments are created with a mixture of the latest generation software and hardware, allowing users to discover the hidden historical values of built heritage with new levels of interactivity and information.[ES] La divulgación de los valores tangibles e intangibles en el patrimonio construido representan uno de los objetivos más importantes en el campo del Patrimonio Cultural Digital (DCH). En los últimos años, diferentes estudios han demostrado la posibilidad de mejorar el conocimiento de nuestro patrimonio construido a través de la integración de los últimos desarrollos en el campo del levantamiento topográfico 3D, modelado 3D, Modelado de Información de la Construcción (BIM) y Realidad eXtendida (RX). Del mismo modo, se ha comprobado que este flujo de trabajo requiere de una gran cantidad de datos y un enfoque holístico con el fin de alcanzar un nivel alto de información compartida entre las diferentes disciplinas y sectores involucrados, como por ejemplo, la restauración, la geomática, los museos virtuales 3D y la industria del videojuego. Junto con el software de entretenimiento y los videojuegos, esta investigación muestra los principales resultados obtenidos durante el proceso de generación de entornos digitales orientados a mejorar el nivel de información y el contenido de uno de los monumentos más importantes en Lombardía: la Basílica de Sant’Ambrogio en Milán. Este estudio, que se inicia con el levantamiento 3D y la toma de datos provenientes de los registros históricos de la iglesia, está orientado a mejorar la creación de una experiencia de realidad extendida, con el objetivo de alcanzar varios niveles de interactividad a través de diferentes dispositivos (escritorio, móvil, auriculares de realidad virtual) y usuarios (expertos, no-expertos).Banfi, F.; Brumana, R.; Stanga, C. (2019). Realidad extendida y modelos informativos en patrimonio arquitectónico: del proceso scan-to-BIM a la realidad virtual y aumentada. Virtual Archaeology Review. 10(21):14-30. https://doi.org/10.4995/var.2019.11923SWORD14301021Attar, R., Hailemariam, E., Glueck, M., Tessier, A., McCrae, J., & Khan, A. (2010). BIM-based building performance monitor. Invited Video at Symposium on Simulation for Architecture and Urban Design (SimAUD) 2010. Orlando, FL, USA. Retrieved from https://www.autodeskresearch.com/publications/bimdashboardvideoAzhar, S. (2011). Building Information Modeling (BIM): Trends, benefits, risks, and challenges for the AEC industry. Leadership and management in engineering, 11(3), 241-252. https://doi.org/10.1061/(ASCE)LM.1943-5630.0000127Banfi, F. (2016). Building Information Modelling-A novel parametric modeling approach based on 3D surveys of historic architecture. In Euro-Mediterranean Conference (pp. 116-127). Cham: Springer. https://doi.org/10.1007/978-3-319-48496-9_10Banfi, F. (2017). BIM orientation: grades of generation and information for different type of analysis and management process. International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences, 42(2/W5), 57-64. 10.5194/isprs-archives-XLII-2-W5-57-2017Banfi, F. (2019). Holistic generative modeling process for HBIM (Doctoral dissertation, Politecnico di Milano, Italy).Banfi, F., Chow, L., Ortiz, M. R., Ouimet, C., & Fai, S. (2018). Building Information Modeling for cultural heritage: The management of generative process for complex historical buildings. In M. Ioannides, E. Fink, R. Brumana, P. Patias, A. Doulamis, J. Martins, M. Wallace (Eds.), Digital Cultural Heritage (pp. 119-130). Cham: Springer. https://doi.org/10.1007/978-3-319-75826-8_10Barazzetti, L., Banfi, F., Brumana, R., Gusmeroli, G., Previtali, M., & Schiantarelli, G. (2015). Cloud-to-BIM-to-FEM: Structural simulation with accurate historic BIM from laser scans. Simulation Modelling Practice and Theory, 57, 71-87. https://doi.org/10.1016/j.simpat.2015.06.004Blanco-Pons, S., Carrión-Ruiz, B., Lerma, J. L., & Villaverde, V. (2019). Design and implementation of an augmented reality application for rock art visualization in Cova dels Cavalls (Spain). Journal of Cultural Heritage. https://doi.org/10.1016/j.culher.2019.03.014Bonavita, A. (2004). Pellegrino Tibaldi a Milano: i lavori alla cupola e al coro della basilica di Sant'Ambrogio. Arte Lombarda,140(1), 89-91.Bradley, A., Li, H., Lark, R., & Dunn, S. (2016). BIM for infrastructure: An overall review and constructor perspective. Automation in Construction, 71, 139-152. https://doi.org/10.1016/j.autcon.2016.08.019Brumana, R., Condoleo, P., Grimoldi, A., & Previtali, M. (2019). Towards a semantic based hub platform of vaulted systems: HBIM Meets A GeoDB. International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences, 42(2/W11), 301-308. https://doi.org/10.5194/isprs-archives-XLII-2-W11-301-2019Brumana, R., Condoleo, P., Grimoldi, A., Banfi, F., Landi, A. G., Previtali, M., (2018a). HR LOD based HBIM to detect influences on geometry and shape by stereotomic construction techniques of brick vaults. Applied Geomatics, 10(4), 529-543. https://doi.org/10.1007/s12518-018-0209-3Brumana, R., Condoleo, P., Grimoldi, A., Landi, A. G., Attico, D., Turrina, A., & Banfi, F. (2018b). HBIM Feeding Open Access Vault Inventory Through GeoDB HUB. In M. Ioannidess, E. Fink, R. Brumana, P. Patias, A. Doulamis, J. Martins, M. Wallace (Eds.), Digital Heritage (pp. 27-38). Cham: Springer. https://doi.org/10.1007/978-3-030-01762-0_3Brumana, R., Della Torre, S., Previtali, M., Barazzetti, L., Cantini, L., Oreni, D., & Banfi, F. (2018c). Generative HBIM modelling to embody complexity (LOD, LOG, LOA, LOI): surveying, preservation, site intervention-the Basilica di Collemaggio (L'Aquila). Applied Geomatics, 10(4), 545-567. https://doi.org/10.1007/s12518-018-0233-3Cabrelles, M., Blanco-Pons, S., Carrión-Ruiz, B., & Lerma, J. L. (2018). From multispectral 3D recording and documentation to development of mobile apps for dissemination of cultural heritage. In T. Levy, I. Jones (Eds.), Cyber-Archaeology and Grand Narratives (pp. 67-90). Cham: Springer. https://doi.org/10.1007/978-3-319-65693-9_5Cassina, F. (1844). Le fabbriche più cospicue di Milano. Milan: Domenico Pedrinelli (book consulted at the Historical Library, Politecnico di Milano).Castellazzi, G., D'Altri, A., Bitelli, G., Selvaggi, I., & Lambertini, A. (2015). From laser scanning to finite element analysis of complex buildings by using a semi-automatic procedure. Sensors, 15(8), 18360-18380. https://doi.org/10.3390/s150818360De Dartein, F. (1865-82). Etude sur l'architecture lombarde et sur les origines de l'architecture romano-bizantine. Paris: Dunod (book consulted at the Historical Library, Politecnico di Milano).Degli Abbati, S., D'Altri, A. M., Ottonelli, D., Castellazzi, G., Cattari, S., de Miranda, S., & Lagomarsino, S. (2019). Seismic assessment of interacting structural units in complex historic masonry constructions by nonlinear static analyses. Computers & Structures, 213, 51-71. https://doi.org/10.1016/j.compstruc.2018.12.001Gatti Perrer, M. L. (1995). Il tempio ininterrotto. Milan: Vita e Pensiero.Georgopoulos, A., (2018). Contemporary Digital Technologies at the Service of Cultural Heritage. In B. Chanda, S. Chaudhuri, S. Chaudhury (Eds.), Heritage Preservation (pp. 1-20). Singapore: Springer. https://doi.org/10.1007/978-981-10-7221-5_1Gusmeroli, G., & Schiantarelli, G., (2014). From laser clouds to BIM and finite element analysis: the case study of Castel Masegra (Master thesis, Politecnico di Milano, Italy).Ioannides, M., Magnenat-Thalmann, N., & Papagiannakis, G. (Eds.) (2017). Mixed Reality and Gamification for Cultural Heritage. Cham: Springer. https://doi.org/10.1007/978-3-319-49607-8Korumaz, M., Betti, M., Conti, A., Tucci, G., Bartoli, G., Bonora, V., Güleç Korumaz, A., & Fiorini, L. (2017). An integrated Terrestrial Laser Scanner (TLS), Deviation Analysis (DA) and Finite Element (FE) approach for health assessment of historical structures. A minaret case study. Engineering Structures, 153(15), 224-238. https://doi.org/10.1016/j.engstruct.2017.10.026Kumar, S. S., & Cheng, J. C. (2015). A BIM-based automated site layout planning framework for congested construction sites. Automation in Construction, 59, 24-37. https://doi.org/10.1016/j.autcon.2015.07.008Lai, Z., Hu, Y. C., Cui, Y., Sun, L., & Dai, N. (2017). Furion: Engineering high-quality immersive virtual reality on today's mobile devices. Proceedings of the 23rd Annual International Conference on Mobile Computing and Networking (pp. 409-421). Snowbird, Utah, USA. https://doi.org/10.1145/3117811.3117815Landriani, G. (1889). La basilica ambrosiana fino alla sua trasformazione in chiesa lombarda a volte. Milan: Hoepli.Lerma, J. L., Navarro, S., Cabrelles, M., & Villaverde, V. (2010). Terrestrial laser scanning and close range photogrammetry for 3D archaeological documentation: the Upper Palaeolithic Cave of Parpalló as a case study. Journal of Archaeological Science, 37(3), 499-507. https://doi.org/10.1016/j.jas.2009.10.011Lusuardi Siena, S. (1997). Ambrogio, il costruttore sapiente. In M. Rizzi, C. Pasini, M. P. Rossignani (Eds.), La città e la sua memoria: Milano e la tradizione di Sant'Ambrogio (pp. 34-35). Milan: Electa.Mazzetti, P., Latre, M. Á., Ernst, J., Brumana, R., Brauman, S., Nativi, S. ( 2015). Virtual hubs for facilitating access to open data. EGU General Assembly Conference held 12-17 April, 2015 in Vienna, Austria.Obidah, R., & Bein, D. (2019). Game based learning using unreal engine. In S. Latifi (Eds.), 16th International Conference on Information Technology-New Generations (pp. 513-519). Cham: Springer. https://doi.org/10.1007/978-3-030-14070-0_72Oreni, D., Della Torre, S., Brumana, R., & Banfi, F. (2017). Disegno e modellazione parametrica per la conservazione di un edificio monumentale danneggiato da un evento sismico. Dal rilievo al cantiere. ANANKE Speciale Geores 2017, 153-158.Oreni, D., Brumana, R., Della Torre, S., Banfi, F., & Previtali, M. (2014). Survey turned into HBIM: the restoration and the work involved concerning the Basilica di Collemaggio after the earthquake (L'Aquila). ISPRS Annals of the Photogrammetry, Remote Sensing and Spatial Information Sciences, 2(5), 267-273. https://doi.org/10.5194/isprsannals-II-5-267-2014Patetta, L. (2001). Bramante e la sua cerchia a Milano e in Lombardia 1480-1500. Milan: Skira.Peroni, A. (1987). Tradizione e innovazione in S. Ambrogio romanico. In C. Bertelli (Eds.), Il Millennio Ambrosiano, (pp. 156-172). Milan: Electa.Piegl, L., & Tiller, W. (2012). The NURBS book. Springer Science & Business Media. Cham: Springer.Previtali, M., Barazzetti, L., Banfi, F., & Roncoroni, F. (2019). Informative content models for infrastructure load testing management: the Azzone Visconti Bridge in Lecco. International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences, 42(2/W11), 995-1001. https://doi.org/10.5194/isprs-archives-XLII-2-W11-995-2019Reggiori, F. (1949). Superstantia Sancti Abrosii Mediolani. Milan: A. Faccioli.Rossi, F. M. (1884). Cronaca dei ristauri e delle scoperte fatte nell'insigne basilica di S. Ambrogio dall'anno 1857 al 1876. Milan: Tipografia di S. Giuseppe.Santana Quintero, M., Blake, B., Eppich, R., & Ouimet, C. (2008). Heritage documentation for conservation: partnership in learning. In 16th ICOMOS General Assembly and International Symposium: 'Finding the spirit of place - between the tangible and the intangible'. Quebec, Canada.Stanga, C., Spinelli, C., Brumana, R., Oreni, D., Valente, R., Banfi, F. (2017). A N-D virtual notebook about the Basilica of S. Ambrogio in Milan: information modeling for the communication of historical phases subtraction process. International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences, 42(2/W5), 653-660. https://doi.org/10.5194/isprs-archives-XLII-2-W5-653-2017Statham, N. (2019). Scientific rigour of online platforms for 3D visualization of heritage. Virtual Archaeology Review, 10(20), 1-16. https://doi.org/10.4995/var.2019.9715Summa, A. (1995). La scultura medioevale nella basilica di S. Ambrogio. In M. L. Gatti Perrer (Eds.), La basilica di S. Ambrogio: il tempio ininterrotto. Milan: Vita e pensiero.Tucci, G., & Lerma, J. L. (2018). Special Issue GEORES2017. Geomatics and restoration: Conservation of cultural heritage in the Digital Era. Preface. Applied Geomatics, 10(4), 277-278. https://doi.org/10.1007/s12518-018-0247-xVolk, R., Stengel, J., & Schultmann, F. (2014). Building Information Modeling (BIM) for existing buildings - Literature review and future needs. Automation in construction, 38, 109-127. https://doi.org/10.1016/j.autcon.2013.10.023Wetzel, E. M., & Thabet, W. Y. (2015). The use of a BIM-based framework to support safe facility management processes.Automation in Construction, 60, 12-24. https://doi.org/10.1016/j.autcon.2015.09.00
Matrix metalloproteinase and heart failure : is it time to move from research to clinical laboratories?
No full textSurfactant protein B : From biochemistry to its potential role as diagnostic and prognostic marker in heart failure
No full textGrowing interest raised on circulating biomarkers of structural alveolar–capillary unit damage and very recent data support surfactant protein type B (SP-B) as the most promising candidate in this setting. With respect to other proteins proposed as possible markers of lung damage, SP-B has some unique qualities: it is critical for the assembly of pulmonary surfactant, making its lack incompatible with life; it has no other known site of synthesis except alveolar epithelial cells different from other surfactant proteins; and, it undergoes a proteolytic processing in a pulmonary-cell-specific manner. In the recent years circulating SP-B isoforms, mature or immature, have been demonstrated to be detectable in the circulation depending on the magnitude of the damage of alveolar capillary membrane. In the present review, we summarize the recent knowledge on SP-B regulation, function and we discuss its potential role as reliable biological marker of alveolar capillary membrane (dys)function in the context of heart failure
Stabilisation of proteolytic enzymes using different carrier systems
No full textV okviru magistrskega dela smo proučevali učinek kapsulacije proteolitičnega encima tripsina na njegovo stabilnost pri različnih temperaturah in pH. Pri tem smo uporabili dva naravna, biorazgradljiva nosilna sistema, in sicer alginat in lipide. V prvi fazi eksperimentalnega dela smo se osredotočili na kapsulacijo tripsina v alginatne mikrokapsule, kjer smo s spreminjanjem parametrov skušali doseči kar najvišjo kapsulacijsko učinkovitost. Ta je za alginat znašala od 1,3 do 2,9 %, kar je bistveno prenizka učinkovitost, tako za raziskovalno kot industrijsko uporabo. Učinka kapsulacije na stabilnost tripsina za alginat zaradi nizke kapsulacijske učinkovitosti nismo preučevali. V drugi fazi pa smo se osredotočili na kapsulacijo tripsina v liposome, kjer smo uspeli kapsulirati 54 % celokupnega tripsina, prisotnega v kapsulacijski mešanici. Tako pripravljene vzorce kapsuliranega tripsina smo izpostavili različnim kombinacijam temperature (4, 25 in 37 °C) in pH (7,0 in 9,0). Za ovrednotenje učinka kapsulacije na stabilnost tripsina smo za kontrolo pripravili vzorce prostega encima. Pri tem smo ugotovili, da kapsuliran tripsin obdrži večji delež aktivnosti, kot prost tripsin pri temperaturi 4 in 25 °C ter pri obeh pH vrednostih. Pri temperaturi 37 °C tega učinka nismo zaznali oz. je bil zanemarljiv.We examined the effects of encapsulation on the stability of a proteolytic enzyme trypsin to different combinations of pH and temperature. For encapsulation, we used alginate and lipids, two types of natural, biodegradable polymers. In the first part of this study we focused on the encapsulation of trypsin into alginate microbeads. By varying the parameters of the encapsulation process we were looking to achieve as high encapsulation efficiency as possible. Using alginate we achieved between 1,3 and 2,9 % efficiency, which is far too low for any research or industrial application. As a result of low encapsulation efficiency, we did not evaluate the effect on encapsulation in alginate on the stability of trypsin. In the second stage our research was focused on encapsulation of trypsin into liposomes, where we achieved 54 % encapsulation efficiency. We exposed the prepared samples of encapsulated trypsin to various combinations of pH (7.0 and 9.0) and temperatures (4, 25 and 37 °C). To evaluate the effect of encapsulation on the stability of trypsin, we prepared samples of free enzyme in the same manner. We observed an increase in stability of encapsulated trypsin at temperatures of 4 and 25 °C at both pH values. This effect was not observed with the samples incubated at 37 °C, i.e. the effect was negligible
Enantiospecific and diastereoselective synthesis of C11-C17 fragment of tylonolide from “asymmetrized tris (hydroxymethyl)methane”
No full textThe fragment C-11-C-17 of tylonolide 2 was straightforwardly and stereoselectively synthesized starting from monoacetate 5, which is a synthetic equivalent of ''asymmetrized tris (hydroxymethyl)methane'' 3
Signaling pathways involved in the induction of PAI-1 by VLDL in HEPG2 cells
No full textWe have previously shown that very low density lipoproteins (VLDL) enhance the biosynthesis of plasminogen activator inhibitor type 1 (PAl-1) in HepG2 cells. In this study we have investigated the mechanism(s) responsible for the induction of PAI-1 biosynthesis by this lipoprotein fraction. To this end subconfluent HepG2 cells were incubated for 16 h with 100 μg/ml VLDL in the presence of different inhibitors of signalling pathways. Mepacrine (15μM), a phospholipase inhibitor, completely prevented the enhancing effect of VLDL on PAI-I secretion. Protein kinase C involvement was investigated using a specific inhibitor (H7, 50μM) or by enzyme downregulation by cell pretreatment with PMA (100nM). In these conditions VLDL induced PAI-1 biosynthesis was reduced by 80% and 40%, respectively. The role of calcium was investigated by the use of specific inhibitors added to cell cultures before VLDL. TMB8 (20 μM), which prevents Ca2+ release from intracellular stores, reduced PAI-1 secretion by 30%, whereas EGTA (1mM) plus thapsigargin (1-2μM), which induce Ca2+ depletion from internal membrane stores, inhibited it by 50%. In contrast, removal of calcium from the cell culture medium with EGTA (1mM), or blocking ions influx with Nifedipine (50μM) did not not prevent P AI-1 induction by VLDL. Overall the data indicate that several secondary messenger-generating pathways, as the phospholipases, protein kinase C and the release of calcium from internal membrane stores form networks of coregulation which result in the induction of PAI-1 biosynthesis by VLDL
In vitro photodynamic treatment of cancer cells induced by aza-BODIPYs
No full textTwo aza-BODIPY photosensitizers (PSs, compounds 7 and 8), featuring an iodine atom on each pyrrolic unit of their structure, were synthesized in fairly good yields starting from commercial products and tested in vitro on two human cancer cell lines (HCT116 and SKOV3) to assess their photodynamic efficacy. After treating the cell cultures with variable concentrations of 7 or 8 and incubating for the desired incubation time, the cells were irradiated for two hours with a red-light emitting diode (LED) device; afterwards the extent of cell death was determined by MTT assay. Besides the killing effect, the new PSs were also studied to determine further parameters related to photodynamic efficacy, such as the resistance towards photobleaching, the rate of singlet oxygen production, the fluorescence quantum yields, the cellular uptake and the localization inside the cells and, finally, flow cytometric analysis for apoptosis. Considering the results as a whole, these aza-BODIPYs can be considered to be promising photosensitizers because of their IC50 values being below micromolar concentrations and for more rather interesting features. Actually, these molecules have proved to be: (a) quite stable towards photobleaching; (b) good producers of singlet oxygen and (c) highly penetrating the cells with a wide distribution in the cytosol. Furthermore, in accordance with the good rate of singlet oxygen production, the apoptotic cells reach 30% and this allows us to assume a low inflammatory effect of the in vivo PDT treatment; thus a possible in vivo application of these aza-BODIPYs might be plausible
- …
